Dataset: P. homomalla development (coarse)

Name: Counts of individuals and cell division for 100 Plexaura homomalla embryos from fertilization to 24 hours after fertilization from adult corals collected in Round Bay, St. John, USVI in July of 2019
Published: 2021-07-15
Keywords: cleavage polyembryony, Cnidaria, ex situ rearing, Octocorallia, Plexauridae, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.855766.1

Spatial Extent: N:18.345 E:-64.681 S:18.345 W:-64.681

Temporal Extent: 2019-07-20

Project:

Collaborative Research: Pattern and process in the abundance and recruitment of Caribbean octocorals (Octocoral Community Dynamics)

Principal Investigator:

Howard Lasker (State University of New York at Buffalo, SUNY Buffalo)

Scientist:

Kaitlyn Tonra (State University of New York at Buffalo, SUNY Buffalo)

Christopher Wells (State University of New York at Buffalo, SUNY Buffalo)

Contact:

Kaitlyn Tonra (State University of New York at Buffalo, SUNY Buffalo)

Technician:

Emily Anderson (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-07-15

Restricted:

Release Date:

2021-09-01

Validated:

Yes

Current State:

Final no updates expected

Counts of individuals and cell division for 100 Plexaura homomalla embryos from fertilization to 24 hours after fertilization from adult corals collected in Round Bay, St. John, USVI in July of 2019

Abstract:

Counts of individuals and cell division for 100 Plexaura homomalla embryos from fertilization to 24 hours after fertilization. Adult corals used in this study were collected in Round Bay, St. John, USVI on July 14–15, 2019. The results publication for this dataset is Tonra et al. (2021, see Fig. 4).

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-07-15	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.855766.1	2021-09-14

Methods & Sampling

Methodology:

Eleven female and four male branches of Plexaura homomalla were collected from adult colonies on an octocoral-dominated reef in Round Bay, St. John, USVI on July 14–15, 2019. All branches were about 15 cm long. Colonies were determined to be gravid in the field by cutting a 5-cm piece diagonally and looking for spermaries or eggs. Colonies were transported to the Virgin Islands Environmental Research Station and maintained in a sea table.

We monitored for signs of spawning for 5 hr starting at sunset starting one night after the full moon from July 17–23, 2019. Eggs and sperm were collected together with 60- mL syringes as they were released from the colonies and were stored in water from the tank for 30 min to ensure fertilization. We refer to the time at which gametes were collected as t = 0. On the second night of spawning, 100 eggs were placed in an isolated container and the number of cells was counted in triplicate by two different observers. Counts were taken every 0.5 hr from 2.5 to 7 hr after fertilization, hourly until 10 h after fertilization, and at 15, 18, and 24 hr after fertilization.

Instruments:
dissecting microscope, hand lens

Data Processing Description

BCO-DMO data manager processing notes:

* Data from file NSF.Phomomalla_development_coarse_monitoring.xlsx sheet "data" imported into the BCO-DMO data system.
* Added column ISO_DateTime_UTC_Start from times and dates provided in local time UTC-4.

Data Files

File
p_homomalla_dev_coarse.csv (Comma Separated Values (.csv), 3.12 KB) MD5:b69f6a0dd09c2a71dbba3b05de2e92fa Primary data file for dataset ID 855766

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1756381

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
container	monitoring dish containing the eggs (either A or B). after hour 7, containers A and B were split to ease tracking of developing eggs (A1 and A2 or B1 and B2)	unitless	sample_descrip
start_time	start time of that counting period in format HH:MM (in Atlantic Standard time zone, UTC-4) Format: %H:%M	unitless	time_local
end_time	end time for that counting period in format HH:MM (in Atlantic Standard time zone, UTC-4) Format: %H:%M	unitless	time_local
time	time relative to the average time corals were spawning and eggs were fertilized (i.e., 22:08) in hours	unitless	time_elapsed
date	date of observation (Altantic Standard Time, UTC-4) Format: %m-%d-%y	unitless	date_local
cell_1	number of eggs/embryos that were 1 cell	unitless	count
cell_2	number embryos that were 2 cells	unitless	count
cell_4	number embryos that were 4 cells	unitless	count
cell_8	number embryos that were 8 cells	unitless	count
cell_16	number embryos that were 16 cells	unitless	count
cell_32	number embryos that were 32 cells	unitless	count
cell_64	number embryos that were 64 or more cells	unitless	count
total	total number of eggs/embryos	unitless	count
ISO_DateTime_UTC_Start	start Datetime of that counting period in ISO 8601 format yyyy-mm-ddTHH:MMZ (UTC). Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC

Status message

Database

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Dataset: P. homomalla development (coarse)