Dataset: Growth behaviors of diatom monoculture and diatom-bacteria co-cultures in the lab

ValidatedRelease Date:2023-07-21Final no updates expectedDOI: 10.26008/1912/bco-dmo.856200.1Version 1 (2022-04-06)Dataset Type:experimental

Principal Investigator, Contact: Kimberly Halsey (Oregon State University)

Co-Principal Investigator: Stephen Giovannoni (Oregon State University)

BCO-DMO Data Manager: Taylor Heyl (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Interactions between phytoplankton and bacterioplankton mediated by volatile organic compounds (Plankton Interactions and VOC)


Abstract

This dataset represents the growth behaviors of diatom (Phaeodactylum tricornutum) monoculture and diatom-bacteria co-cultures in the lab.

This dataset represents the growth behaviors of diatom (Phaeodactylum tricornutum) monoculture and diatom-bacteria co-cultures in the lab.

The cultures described in the dataset were grown as follows:
Cultures were inoculated in f/2+Si media and grown under 60 micro-einsteins, 12 hours light:12 hour dark cycles at 19 degrees Celsius. Sampling for VOCs was done in triplicate during exponential and stationary growth phases. Exponential phase sampling was done when the cell density of P. tricornutum was 2-4 * 10^5 per milliliter (ml) and at 6-7 hours after dawn. Stationary phase sampling was done when the cell density of P. tricornutum was 2-4 * 10^6 per milliliter (ml) at 6-7 hours after dawn.

Cell densities were measured by a particle counter (Coulter) or Guava flow cytometry.


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