Dataset: Temperature

Name: Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata
Published: 2021-08-10
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.858081.1

Spatial Extent: N:29.55723 E:34.951579 S:29.55723 W:34.951579

Temporal Extent: 2019-01 - 2019-02

Project:

EAGER: Collaborative Research: Bleaching phenotypes of acute vs. chronic coral bleaching susceptibility and resilience: towards a standardized coral resilience diagnostic (EAGER-CBASS)

Principal Investigator:

Daniel J. Barshis (Old Dominion University, ODU)

Co-Principal Investigator:

Mark E. Warner (University of Delaware)

Scientist:

Nicolas R. Evensen (Old Dominion University, ODU)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-08-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata

Abstract:

This dataset includes temperature data (in degrees Celsius) measured during two experiments: an 18 h acute thermal stress assay using the Coral Bleaching Automated Stress System (CBASS) and a 14 day chronic heat stress experiment using the Red Sea Simulator (RSS). Experiments were performed in Eilat, Israel in January-February 2019.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-08-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.858081.1	2021-08-11

Methods & Sampling

Two experiments were conducted to test the effects of acute (18 h) and chronic (14 d) thermal stress on the scleractinian coral, Stylophora pistillata. A full description of the methodology is detailed in the published manuscript Evensen et al. (2021).

Multiple ramets of 8 Stylophora pistillata genets were used in two experiments: an 18 h acute thermal stress assay using the Coral Bleaching Automated Stress System (CBASS) and a 14 day chronic heat stress experiment using the Red Sea Simulator (RSS). Experiments were performed in Eilat, Israel in January-February 2019.

In each experiment, corals were exposed to four temperature treatments (27°C (summer ambient), 29.5°C, 32°C, and 34.5°C), with physiological responses of the coral and symbionts assessed at the end of the thermal stress exposure (T1) and after a period of recovery (T2). This dataset represents the temperature data measured during the acute (CBASS) and chronic heat (RSS) stress experiments.

Physiological responses of the coral host and associated endosymbionts were measured at the end of the heating hold (after 6 h in the CBASS and 6-10 days in the RSS), referred to as T1, and after a recovery period (12 h and 3-7 days post-heat stress in the CBASS and RSS, respectively), referred to as T2. Control/baseline samples were also collected for each genet - a 'field' sample was assessed upon returning from sample collection and a 'T0' sample was assessed after ~ 2 h incubation in the experimental tanks, immediately prior to the start of the experiments, during which time seawater temperatures in the tanks was steadily ramped up from 22°C to 27°C. Experimental tanks were then ramped up to temperature treatments reaching 27°C, 29.5°C, 32°C, and 34.5°C, with each temperature containing two replicate tanks (A and B).

Problem report:
Missing data were samples that were not measured or that are missing due to sample mortality.

Data Processing Description

Data Processing:
Data were organized and plotted using R statistical software (version 4.0.3) or in Prism (v. 8.0; GraphPad Software, Inc.).

BCO-DMO Processing:
- renamed fields to comply with BCO-DMO naming conventions;
- changed date/time field to ISO8601 format.

The originally submitted GitHub repository https://github.com/BarshisLab/CBASS-vs-RSS-Physiology was forked to https://github.com/BCODMO/CBASS-vs-RSS-Physiology and tagged with release 1.0, which corresponds with this dataset submission. The original repository may have continued updates.

Data Files

File
temperature.csv (Comma Separated Values (.csv), 99.69 KB) MD5:83f6e73995f4d49abe42621755932a57 Primary data file for dataset ID 858081

Supplemental Files

File
CBASS-vs-RSS-Physiology-1.0.zip (ZIP Archive (ZIP), 10.18 MB) MD5:c0ee937e896b43e0224439a51f6f6358 Code used to analyze and plot data; associated with BCO-DMO datasets 858036 and 858081 and with Evensen et al., 2021 (doi: 10.1002/lno.11715) These files are also available in the following GitHub repository: https://github.com/BCODMO/CBASS-vs-RSS-Physiology/releases/tag/1.0

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1833201
NSF Division of Ocean Sciences	OCE-1833215

Deployments

None available yet

Instruments

Aquarium

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Aquarium

Generic Description:

Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept

Aquarium chiller

Supplied Name: IceProbe Thermoelectric chillers

Supplied Description:

Temperature control in each tank is achieved using a combination of IceProbe Thermoelectric chillers (Nova Tec) and 150-200 W aquarium heaters linked to a custom-built controller (Arduino MEGA 2560).

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Centrifuge

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Centrifuge

Generic Description:

A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.

Coral Bleaching Automated Stress System

Supplied Name: CBASS

Supplied Description:

Instrument Type

Generic Name: Coral Bleaching Automated Stress System

Acronym: CBASS

Generic Description:

CBASS, which stands for "Coral Bleaching Automated Stress System", are portable, field-deployable experimental tanks used to apply rapid, acute heat stress challenges. This system is described in:

Voolstra, C. R., Buitrago‐López, C., Perna, G., Cárdenas, A., Hume, B. C. C., Rädecker, N., & Barshis, D. J. (2020). Standardized short‐term acute heat stress assays resolve historical differences in coral thermotolerance across microhabitat reef sites. Global Change Biology, 26(8), 4328-4343. Portico. https://doi.org/10.1111/gcb.15148

Flow Cytometer

Supplied Name: MACSQuant Analyzer 10, Miltenyi Biotec

Supplied Description:

Instrument Type

Generic Name: Flow Cytometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Generic Description:

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Homogenizer

Supplied Name: Diax 900, Heidolph Instruments

Supplied Description:

Instrument Type

Generic Name: Homogenizer

Generic Description:

A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.

LED light

Supplied Name: GalaxyHydro, Roleandro

Supplied Description:

Tanks were located indoors and received ~ 300 μmol quanta m−2 s−1 from LED aquarium lights (GalaxyHydro, Roleandro) for 12 h per day, as measured via a Li-Cor spherical quantum PAR (Photosynthetically Active Radiation) sensor (LI-192, Li-COR).

Instrument Type

Generic Name: LED light

Generic Description:

A light-emitting diode (LED) is a semiconductor light source that emits light when current flows through it. Electrons in the semiconductor recombine with electron holes, releasing energy in the form of photons.

LI-COR LI-192 PAR Sensor

Supplied Name: LI-192, Li-COR

Supplied Description:

Instrument Type

Generic Name: LI-COR LI-192 PAR Sensor

Acronym: LI-COR LI-192 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/

Generic Description:

The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.

These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.

The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com)

Oxygen Sensor

Supplied Name: FireSting O2, Pyroscience oxygen probes

Supplied Description:

Oxygen flux in the chambers was measured at 1-s intervals using oxygen probes (FireSting O2, Pyroscience).

Instrument Type

Generic Name: Oxygen Sensor

Acronym: Dissolved Oxygen Sensor

Generic Description:

An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed

plate reader

Supplied Name: Biotek HT Synergy plate reader

Supplied Description:

Instrument Type

Generic Name: plate reader

Generic Description:

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Experiment	experiment: CBASS = Coral Bleaching Automated Stress System; RSS = Red Sea Simulator	unitless	exp_type
DateTime	date and time (local time zone: UTC/GMT+3:00); format: YYYY-MM-DDThh:mm Format: %Y-%m-%dT%H:%M	unitless	ISO_DateTime_Local
Tank	tank ID	unitless	tank
Temperature	temperature; RSS temperatures are averaged over 1 h and CBASS temperatures are averaged over 15 mins	degrees Celsius	temp

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Dataset: Temperature