Dataset: Intracellular metabolites from an experimental manipulation of marine microorganisms from samples collected during R/V Knorr cruise KN210-04 in May of 2013

ValidatedFinal no updates expectedVersion 1 (2021-08-16)Dataset Type:Cruise Results

Principal Investigator: Elizabeth Kujawinski (Woods Hole Oceanographic Institution)

Scientist: Krista Longnecker (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)

Project: The Metabolic Response of Coastal Bacteria to Mortality-Derived Phytoplankton Dissolved Organic Matter (MortalityDOM)

Abstract

Intracellular metabolites from an experimental manipulation of marine microorganisms. Seawater used in the experiments was collected off the northeastern corner of South America at 9.75 N, 55.3 W from 70 m. The marine environment holds one of the largest pools of reduced organic carbon on Earth. Within this organic carbon are metabolites produced as a result of microbial activity. This project considers the effects of predation and viral lysis on the metabolites found in the surface ocean. ...

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Cite Dataset

Raw Spectral Data Files are available in the MetaboLights database under study ID "MTBLS461" (https://www.ebi.ac.uk/metabolights/MTBLS461/).  Sample, assay, and metabolite information in ISAtab format are also available from that MetaboLights study.

Related Datasets

IsRelatedTo
Dataset: https://www.ebi.ac.uk/metabolights/MTBLS461/
Longnecker, K., Kujawinski, E. (2017) MTBLS461: Intracellular metabolites from an experimental manipulation of marine microorganisms. MetaboLights Database. Release Date: 2017-12-20. Available at https://www.ebi.ac.uk/metabolights/MTBLS461/

Related Publications

Results
Longnecker, K., & Kujawinski, E. B. (2020). Intracellular Metabolites in Marine Microorganisms during an Experiment Evaluating Microbial Mortality. Metabolites, 10(3), 105. doi:10.3390/metabo10030105
Methods
Dittmar, T., Koch, B., Hertkorn, N., & Kattner, G. (2008). A simple and efficient method for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawater. Limnology and Oceanography: Methods, 6(6), 230–235. doi:10.4319/lom.2008.6.230
Methods
Kuhl, C., Tautenhahn, R., Böttcher, C., Larson, T. R., & Neumann, S. (2011). CAMERA: An Integrated Strategy for Compound Spectra Extraction and Annotation of Liquid Chromatography/Mass Spectrometry Data Sets. Analytical Chemistry, 84(1), 283–289. doi:10.1021/ac202450g
Methods
Rabinowitz, J. D., & Kimball, E. (2007). Acidic Acetonitrile for Cellular Metabolome Extraction fromEscherichia coli. Analytical Chemistry, 79(16), 6167–6173. doi:10.1021/ac070470c
Methods
Sumner, L. W., Amberg, A., Barrett, D., Beale, M. H., Beger, R., Daykin, C. A., … Viant, M. R. (2007). Proposed minimum reporting standards for chemical analysis. Metabolomics, 3(3), 211–221. doi:10.1007/s11306-007-0082-2

Kujawinski, E., Longnecker, K. (2021) Intracellular metabolites from an experimental manipulation of marine microorganisms from samples collected during R/V Knorr cruise KN210-04 in May of 2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-16 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/858654 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation