Dataset: Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019

This dataset has not been validatedRelease Date:2022-07-26Data not availableVersion 1 (2021-11-02)Dataset Type:Cruise Results

Principal Investigator: Solange Duhamel (Columbia University)

Co-Principal Investigator: Julia Diaz (University of Georgia)

Contact: Kahina Djaoudi (University of Arizona)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)


Abstract

Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 from March to April of 2019 in the Northwestern Atlantic from the surface to 50 m depth.

Sampling and analytical procedures:

 Seawater samples for bioavailability factor determinations were collected from Niskin bottles (12 L), at station 1 (two measurements) and 3 (one measurement) along a transect from coastal Georgia to offshore waters. The bioavailability factor was calculated based on changes in PO43-  turnover time. The bioavailability factor (BF) is calculated as follows: BF=TE-TN/ TP-TN (Björkman and Karl, 1994), where TE reflects PO43- turnover time in the DOP amended treatment, TN the PO43- turnover time in the control (no additions) and TP the PO43- turnover time in the treatment amended with Pi. In this study, treatments were amended with polyphosphate (Polyp), adenosine triphosphate (ATP), adenosine monophosphate (AMP) or methyl phosphonate (Mepn).

BF ranges from 0 for an unavailable substrate, to a value of 1 for a DOP model substrate having a bioavailability equal to that of +Pi.

Instruments:  Sampling was performed using Niskin bottles (12 L) mounted on a rosette. Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter. 

Location:  Northwestern Atlantic surface waters. Depth: surface-50 m.

Problem report: BF in station 1 (first determination) were determined in duplicates instead of triplicate in station 1 (second determination) and station 3. Therefore, gaps are filled with NaN in the .mat files.   


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Methods

Björkman, K., & Karl, D. M. (1994). Bioavailability of inorganic and organic phosphorus compounds to natural assemblages of microorganisms in Hawaiian coastal waters. Marine Ecology Progress Series, 111(3), 265–273. http://www.jstor.org/stable/24849565