Dataset: P_Pools In Situ Experiments

Name: Concentrations of particulate phosphate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019
Published: 2021-11-02
Keywords: oceans

View Data: Data not available yet

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Spatial Extent: N:31.7635 E:-79.8421 S:31.0175 W:-80.7965

Temporal Extent: 2019-03-30 - 2019-04-10

Project:

Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)

Principal Investigator:

Solange Duhamel (Columbia University)

Co-Principal Investigator:

Julia Diaz (University of Georgia, UGA)

Contact:

Kahina Djaoudi (University of Arizona, UA)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-02

Restricted:

Yes

Release Date:

2022-07-26

Validated:

Current State:

Data not available

Concentrations of particulate phosphate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019

Abstract:

Concentrations of particulate phosphate in seawater collected during R/V Savannah cruise SAV-19-02 from March to April of 2019 in the Northwestern Atlantic from the surface to 50 m depth.

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Methods & Sampling

Sampling and analytical procedures:

Seawater samples were collected in triplicate from Niskin bottles (12 L), at three stations (St. 1, St. 2 and St. 3) along a transect from coastal Georgia to offshore waters. Latitude (lat) and longitude (lon) of sampling sites are provided in .mat files. Stations 1 and 2 were on the continental shelf (15–30 m depth), while Station 3 was on the shelf break (220 m depth) adjacent to the Gulf Stream. At each station, samples were collected at 2-3 depths between the surface (4–5 m) and 50 m for SRP, DOP and PP. SRP (50 mL), DOP (50 mL) and PP (2 L) were sampled from Niskin bottles. SRP and DOP samples were filtered through 0.2 µm polycarbonate filters and transferred into high density polyethylene bottles (HDPE, 60 mL) and kept frozen (-20°C) until analysis. PP samples were filtered through pre-combusted (450°C, 4.5h) and acid washed (1N HCl) GF/F filters, using a peristaltic pump. PP filters were wrapped in pre-combusted (450°C, 4.5h) aluminum foil, and kept frozen (-20°C) until analysis.

SRP, DOP and PP were analyzed following a spectrophotometric method based on the molybdenum reagent (Murphy and Riley, 1962). SRP concentrations were analyzed using the Liquid Waveguide Capillary Cell® (LWCC, optical length path = 2.5 m). DOP concentrations were determined by subtraction of SRP from the total dissolved phosphate (TDP). TDP was digested to SRP following the wet oxidation procedure (Pujo-Pay and Raimbault, 1994) and SRP was subsequently determined using the LWCC. PP concentrations were determined after high-temperature combustion (450°C, 4.5 h) and HCl extraction (0.5 N) (Strickland and Parsons, 1972). The released SRP following this procedure was then analyzed spectrophotometrically using a conventional spectrophotometer with a 10 cm cuvette.

Location: Northwestern Atlantic surface waters. Depth: surface-50 m.

Instruments: Sampling was performed using Niskin bottles (12 L) mounted on a rosette. Measurements of SRP, DOP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument). PP samples were measured using a Gynesis spectrophotometer.

Data Processing Description

Data were organized using MATLAB and output as .mat files. Gaps in data were filled with NaN in the .mat files.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1737083
NSF Division of Ocean Sciences	OCE-2001212
NSF Division of Ocean Sciences	OCE-1736967
NSF Division of Ocean Sciences	OCE-1948042

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
SAV-19-02	Zephyr Zooming in on Enzymatic PhosphoHYdrolysis Reactions	2019-03-30	R/V Savannah	Julia Diaz (Chief Scientist)	data info

Instruments

Liquid Waveguide Capillary Cells

Supplied Name: Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument).

Supplied Description:

Measurements of SRP, DOP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument).

Instrument Type

Generic Name: Liquid Waveguide Capillary Cells

Acronym: LWCC

Generic Description:

Liquid Waveguide Capillary Cells (LWCC) are optical sample cells that combine an increased optical pathlength (2-500 cm) with small sample volumes. They can be connected via optical fibers to a spectrophotometer with fiber optic capabilities. Similar to optical fibers, light is confined within the (liquid) core of an LWCC by total internal reflection at the core/wall interface. Ultra-sensitive absorbance measurements can be performed in the ultraviolet (UV), visible (VIS) and near-infrared (NIR) to detect low sample concentrations in a laboratory or process control environment. According to Beer’s Law the absorbance signal is proportional to chemical concentration and light path length.

Niskin bottle

Supplied Name:

Supplied Description:

Sampling was performed using Niskin bottles (12 L) mounted on a rosette.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Spectrophotometer

Supplied Name: Gynesis spectrophotometer

Supplied Description:

PP samples were measured using a Gynesis spectrophotometer.

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

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Dataset: P_Pools In Situ Experiments