As a secondary assessment of fecundity, colonies of Acropora cerviconis (various genets) were taken to Mote Marine Laboratory in August 2020 for spawning and ex situ assisted sexual reproduction. From genets that spawned, forty random gamete bundles were collected during spawning and the total number of eggs and sperm per bundle were quantified.

Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Supplemental Files
• Related Publications
• Related Datasets
• Parameters
• Instruments
• Project Information
• Funding

This dataset is part of a larger study of Acropora cervicornis (staghorn) corals studied at Mote Marine Lab’s Elizabeth Moore International Center for Coral Reef Research and Restoration.  The different analyses are listed here, and links to other data from this study can be found in the Related Datasets section below.  

Analyses undertaken include:

1. Total Population (Colony size dataset)
2. Morphometric Assessment (Colony size dataset)
3. Primary Fecundity Analysis (Population subset of Colony size dataset, plus Polyps dataset)
4. Dissections (Oocyte number dataset, Oocyte size dataset)
5. Secondary Fecundity Analysis (this dataset of Gamete Bundles)

See Related Datasets section below for links to above mentioned datasets.

Sampling of Acropora cervicornis coral genotypes took place at Mote Marine Lab's spawning nurseries at Sand Key (24.45782, -81.88595) and Looe Key (24.56257, -81.40009). 

Sampling for Primary Fecundity Analysis was performed on July 3, 2020.  From 5 colonies of every genet, 3 linear branches (~10 cm in length) were sampled using bone cutters from the central portion of each colony (see Colony Size dataset, https://www.bco-dmo.org/dataset/843028). Using a ruler, the number of polyps per one square centimeter was recorded from every branch near the base of the fragment (see Polyps per Area dataset,https://www.bco-dmo.org/dataset/868308).

Treatments with formalin, DI, and HCl solution were done to preserve and decalcify the coral, which were then stored until dissection (see datasets Oocyte Size, https://www.bco-dmo.org/dataset/843067 and Oocyte Number,  https://www.bco-dmo.org/dataset/867314).

Sampling for Secondary Fecundity Analysis (this dataset)
As a secondary assessment of fecundity, five replicate colonies of every genet were brought back to the lab on July 31, 2020 for spawning and ex situ assisted sexual reproduction. From every genet that spawned during August 4-10, 2020 (all of them), 40 random gamete bundles were collected during spawning using a transfer pipet and 50 mL falcon tube. Each gamete bundle was immediately placed into a 2 mL glass vial with 10% formalin for fixation and inverted several times to break up the bundle in each vial. Then, the total number of oocytes and sperm per bundle were counted. Eggs were counted by eye by two independent observers. Replicate sperm counts were recorded using a hemocytometer.  

Problem Report:
Genet 31: In the time between when the parental colonies were morphometrically assessed/sampled in the Sand Key nursery for the primary fecundity analysis and brought into the lab for spawning/sampled for the secondary fecundity analysis, the entire tree for genet 31 snapped off from its anchor and went missing. As such, 5 colonies of genet 31 were brought in from a different spawning nursery and location (Looe Key Nursery: 24.56257, -81.40009). Thus, the fecundity data obtained from the fragments and gamete bundles come from different subpopulations (fragments from Sand Key and gamete bundles from Looe Key).

Genet 41:  From the primary fecundity analysis, it was determined that none of the colonies of genet 41 in the Sand Key nursery were gravid and therefore genet 41 was not included in the secondary fecundity analysis (Gamete Bundles Dataset).

Genets 62 + 63: Due to space limitations during transport of colonies from the field nursery to the lab, only 1 colony of genet 62 was brought in for assisted sexual reproduction and secondary fecundity analysis (Gamete Bundles Dataset).

Data processing:
Data were compiled into Excel (Microsoft Office) and analyzed using R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out". Nonparametric statistical and correlation analyses were conducted.

BCO-DMO Processing:
- separated Latitude and Longitude into separate columns
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
 

 

Coverage: Florida Keys, Summerland Key, FL 24.563595°, -81.278572°

NSF Award Abstract:
Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.

Acropora cervicornis dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify A. cervicornis genotypes resistant to disease, 2) identify A. cervicornis genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on A. cervicornis; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.