Regularly measured tank conditions (pH, Temperature, Salinity). measured three times per week throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment (https://www.bco-dmo.org/dataset/871633). pCO2 was calculated every ~14 days from water samples.
Methodology:
Sampling and analytical procedures:
C. compactum and C. nereostratum cultured for 4 months under 12 pCO2/T treatments in thirty-six, 42-liter aquaria.
Seawater samples were obtained every ~14 days using 250 mL ground-glass-stoppered borosilicate glass bottles for measurement of total alkalinity (TA) and dissolved inorganic carbon (DIC) and other carbonate parameters.
Temp, salinity, pH measured three times weekly.
For buoyant weight, specimens were suspended beneath a balance in an aquarium at 4 cm depth in seawater of constant temperature and salinity.
Specimens were dosed with calcein before the experiment and the vertical growth was determined by measuring the vertical growth from the resulting calcein line to the surface.
Westfield, I., Ries, J. B., Williams, B., Rasher, D. B. (2022) Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment from from 2015-2016 (CorallineAlgaePaleo-pH project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-04-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.872498.1 [access date]
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