Dataset: Smallmouth Grunt Mortality

Name: Smallmouth grunt mortality data after exposure to experimental pH treatments
Published: 2022-07-13
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.876987.1

Temporal Extent: 2013-11 - 2019-08

Project:

Ocean Acidification: Effects on Morphology and Mineralogy in Otoliths of Larval Reef Fish (OA-OTO MIN)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Robert J. Holmberg (University of Massachusetts Boston, UMass Boston)

Co-Principal Investigator:

Bradford Bourque (Roger Williams University, RWU)

Eugene D. Gallagher (University of Massachusetts Boston, UMass Boston)

Robyn E. Hannigan (University of Massachusetts Boston, UMass Boston)

Andrew L. Rhyne (Roger Williams University, RWU)

Michael F. Tlusty (University of Massachusetts Boston, UMass Boston)

Contact:

Robert J. Holmberg

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-07-13

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Smallmouth grunt mortality data after exposure to experimental pH treatments

Abstract:

This dataset reports mortality counts from Haemulon chrysargyreum (Smallmouth grunt) reared in an experimental trial in which subjects were raised under one of four pH treatments (8.10, 7.80, 7.60, 7.30). Survival counts were subtracted from the initial stocking density to calculate mortality counts.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2022-07-13	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.876987.1	2022-09-01

Methods & Sampling

Several clutches of Haemulon chrysargyreum eggs were purchased and shipped from a supplier in Florida, USA, and inspected for quality and development. A clutch was selected, eggs hatched, and larvae distributed among the 20 experimental aquaria at a stocking density of 130 individuals per aquarium. Larvae in each aquarium were subjected to one of 4 seawater pH treatments (8.10, 7.80, 7.60, 7.30) randomly assigned and replicated 5x. Seawater temperature in all aquaria was held constant at 28 C. Aquaria were filled with sterilized natural seawater, and 25% water changes were completed every 48 hrs. Larvae were fed ad libitum with wild copepods from monoculture (Pseudodiaptomus spp.) in a background of live microalgae (Isochrysis spp.). Larvae were reared under experimental conditions for 30 days until the majority achieved settlement competency.

Upon completion of the experimental trial, all surviving fish were removed from each aquarium, euthanized with a lethal dose of tricaine mesylate (MS-222) in seawater, and counted. Survival counts were subtracted from the initial stocking density to calculate mortality counts.

The experimental trial took place between November and December 2013. The otolith morphology, fish length, and fish mortality data were collected over a 5-year span between January 2014 and August 2019.

Data Files

File
grunt_mortality.csv (Comma Separated Values (.csv), 499 bytes) MD5:255760f6fc2a622e61049d31077ed01e Primary data file for dataset ID 876987

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Emerging Frontiers Division	EF-1220480

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
TANK	Aquaria ID	unitless	tank
SETPOINT	Seawater pH Setpoint	pH units	pH
pCO2	Seawater pCO2	microatmospheres (uatm)	pCO2
REMAINING	Surviving Fish Count	fish	count
STOCK	Fish Stocking Density	fish	count
MORT	Perished Fish Count	fish	count

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Dataset: Smallmouth Grunt Mortality