Dataset: Particle bulk d15N values from EXPORTS cruises SR1812 on R/V Sally Ride and RR1813 on R/V Roger Revelle in August to September 2018

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.880376.1Version 1 (2022-09-13)Dataset Type:Cruise Results

Principal Investigator: Hilary G. Close (University of Miami Rosenstiel School of Marine and Atmospheric Science)

Co-Principal Investigator: Brian N. Popp (University of Hawaiʻi at Mānoa)

Student: Paul K. Wojtal (University of Miami Rosenstiel School of Marine and Atmospheric Science)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: EXport Processes in the Ocean from Remote Sensing (EXPORTS)

Project: Collaborative Research: Isotopic Indicators for Mechanisms of Organic Matter Degradation in the Northeast Pacific (EXPORTS) (EXPORTS Isotopes)


Abstract

This dataset includes d15N values of bulk nitrogen from size-fractionated and sinking particles collected on the EXPORTS cruises (SR1812 on R/V Sally Ride and RR1813 R/V Roger Revelle) in August to September 2018.

In situ pump filtration was performed using large volume pumps (WTS-LV; McLane Research Laboratories, Inc.) outfitted with mini-MULVFS (Multiple Unit Large Volume in-situ Filtration System) filter holders (Bishop et al. 2012). Pumps were loaded with filter stacks of either 3 filter pore sizes (51- and 5-um acid cleaned Nitex screens and 1 um pre-combusted quartz fiber filter) or 4 filter pore sizes (51- and 6-um acid cleaned Nitex screens, a double layer of 1 um pre-combusted quartz fiber filters, and a double layer of 0.3 um of pre-combusted glass fiber filters). Samples were frozen at -80 degrees Celsius until laboratory analysis. Sediment trap collection was performed using surface tethered sediment traps (STT) and neutrally buoyant sediment traps (NBST). Particles were collected in polycarbonate tubes with a collection area of 0.0113 m² and triggered to close at the end of the sampling period. Samples were collected in 500 mL of 70 ppt salinity, 0.1% formaldehyde-poisoned brine, buffered to pH 8.5 with borate and overlain with 1‑um filtered surface seawater. After collection and allowing particles to sink to the bottom of the tube on board the ship for an hour, brine was filtered through a 335‑um polyester screen and rotary wet split onto pre-combusted glass fiber filters with a pore size of 0.7 um. Samples were frozen at -80 degrees Celsius until laboratory analysis.

Subsamples were split gravimetrically and packed directly into tin capsules for elemental and isotopic composition.

Analyses were carried out using a Thermo Fisher Scientific Flash Elemental Analyzer (EA) coupled to a MAT 253 Plus Isotope Ratio Mass Spectrometer (IRMS) via a Conflo IV open split interface. The EA oxidation reactor was held at 980 degrees C, the reduction reactor at 650 degrees C, and the gas chromatography column at 65 degrees C.


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Results

Wojtal, P. K., Doherty, S. C., Shea, C. H., Popp, B. N., Benitez‐Nelson, C. R., Buesseler, K. O., Estapa, M. L., Roca‐Martí, M., & Close, H. G. (2023). Deconvolving mechanisms of particle flux attenuation using nitrogen isotope analyses of amino acids. Limnology and Oceanography. Portico. https://doi.org/10.1002/lno.12398
Methods

Buesseler, K. O., Benitez-Nelson, C. R., Roca-Martí, M., Wyatt, A. M., Resplandy, L., Clevenger, S. J., Drysdale, J. A., Estapa, M. L., Pike, S., & Umhau, B. P. (2020). High-resolution spatial and temporal measurements of particulate organic carbon flux using thorium-234 in the northeast Pacific Ocean during the EXport Processes in the Ocean from RemoTe Sensing field campaign. Elementa: Science of the Anthropocene, 8(1). https://doi.org/10.1525/elementa.2020.030
Methods

Estapa, M., Buesseler, K., Durkin, C. A., Omand, M., Benitez-Nelson, C. R., Roca-Martí, M., Breves, E., Kelly, R. P., & Pike, S. (2021). Biogenic sinking particle fluxes and sediment trap collection efficiency at Ocean Station Papa. Elementa: Science of the Anthropocene, 9(1). https://doi.org/10.1525/elementa.2020.00122