Dataset: Microsatellite and chloroplast 23S genotypes of Breviolum sp. symbionts within Orbicella faveolata adults from reefs in the Florida Keys from 2009-2011 (SymBioSys project)

ValidatedRelease Date:2022-11-09Final no updates expectedDOI: 10.26008/1912/bco-dmo.882086.1Version 1 (2022-10-10)Dataset Type:experimentalDataset Type:Other Field Results

Principal Investigator, Contact: Mary Alice Coffroth (State University of New York at Buffalo)

Co-Principal Investigator: Margaret W. Miller (NOAA Southeast Fisheries Science Center)

Co-Principal Investigator: David Sheets (Canisius College)

Student: Noel J. Leigh (State University of New York at Buffalo)

Student: Shelby E. McIlroy (State University of New York at Buffalo)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Project: Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)

Abstract

Microsatellite genotypes and chloroplast 23S genotypes (based on length heteroplasmy in domain V of chloroplast large subunit (cp23S) ribosomal DNA sequences) of Breviolum sp. symbionts within Orbicella faveolata adults in the Florida Keys.

Cite Dataset

Sampling and Analytical Methodology

Tissue samples were collected from the top, middle and bottom portions of adult Orbicella faveolata colonies using the syringe method as described in Correa et al. (2009) and DNA extracted following Coffroth et al. (1992). 

Genotypes of the algal symbionts within the genus Breviolum were characterized using three polymorphic microsatellite loci, B7Sym34, B7Sym36 and CA6.38, which were adapted for use with O. faveolata (Thornhill et al. 2009).

Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).

Locations in the Florida Keys (Table of reef abbreviations)

Reef
Abbreviation

Alligator
AR

Coral Garden
CG

Cheeca Rocks
CR

East Turtle
ET

Grecian Rocks
GR

Looe Key
LK

Sand Island
SI

Tennessee
TR

 

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Related Publications

Results
Coffroth, M. A., Leigh, N. J., McIlroy, S. E., Miller, M. W., & Sheets, H. D. (2022). Genetic structure of dinoflagellate symbionts in coral recruits differs from that of parental or local adults. Ecology and Evolution, 12(9). Portico. https://doi.org/10.1002/ece3.9312
Methods
Coffroth, M. A., Lasker, H. R., Diamond, M. E., Bruenn, J. A., & Bermingham, E. (1992). DNA fingerprints of a gorgonian coral: a method for detecting clonal structure in a vegetative species. Marine Biology, 114(2), 317–325. doi:10.1007/bf00349534
Methods
Correa, A. M. S., Brandt, M. E., Smith, T. B., Thornhill, D. J., & Baker, A. C. (2009). Symbiodinium associations with diseased and healthy scleractinian corals. Coral Reefs, 28(2), 437–448. https://doi.org/10.1007/s00338-008-0464-6
Methods
Santos, S. R., Gutierrez-Rodriguez, C., & Coffroth, M. A. (2003). Phylogenetic identification of symbiotic dinoflagellates via length heteroplasmy in Domain V of chloroplast Large Subunit (cp23S)-Ribosomal DNA Sequences. Marine Biotechnology, 5(2), 130–140. doi:10.1007/s10126-002-0076-z

Coffroth, M. A., Miller, M. W., Sheets, D. (2023) Microsatellite and chloroplast 23S genotypes of Breviolum sp. symbionts within Orbicella faveolata adults from reefs in the Florida Keys from 2009-2011 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.882086.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


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Funded by the U.S. National Science Foundation