Dataset: Breviolum symbiont CP23S genotypes in Orbicella faveolata recruits from 2009-2011 (SymBioSys project)

ValidatedRelease Date:2022-11-09Final no updates expectedDOI: 10.26008/1912/bco-dmo.882097.1Version 1 (2023-01-24)Dataset Type:experimentalDataset Type:Other Field Results

Principal Investigator, Contact: Mary Alice Coffroth (State University of New York at Buffalo)

Co-Principal Investigator: Margaret W. Miller (NOAA Southeast Fisheries Science Center)

Co-Principal Investigator: David Sheets (Canisius College)

Student: Noel J. Leigh (State University of New York at Buffalo)

Student: Shelby E. McIlroy (State University of New York at Buffalo)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Project: Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)

Abstract

Chloroplast 23S genotypes (based on length heteroplasmy in domain V of chloroplast large subunit (cp23S) ribosomal DNA sequences) of Breviolum sp. symbionts within Orbicella faveolata recruits that were outplanted to reefs in the Florida Keys.

Cite Dataset

Sampling and Analytical Methodology: 

Egg-sperm bundles were collected from adult colonies of O. faveolata following the techniques described by Miller (2014).  Larvae were reared at a shore-based lab and settled onto terracotta tiles.  Once the larvae had metamorphosed and attached to the tiles, they were outplanted onto the reef by attachment vertically to a PVC rack approximately 0.2 m above the substrate to allow uptake of algal symbionts. Tiles with recruits were sampled one to three months after outplanting, recruits removed from the tile and preserved individually in 95% ethanol for subsequent molecular analysis. DNA extraction followed Coffroth et al. (1992).    Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).

 

Locations in the Florida Keys with Abbreviations

  • Alligator (AR)
  • Coral Garden (CG)
  • Cheeca Rocks (CR)
  • East Turtle (ET)
  • Grecian Rocks (GR)
  • Looe Key (LK)
  • Sand Island (SI)
  • Tennessee (TR)

 

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Related Publications

Results
Coffroth, M. A., Leigh, N. J., McIlroy, S. E., Miller, M. W., & Sheets, H. D. (2022). Genetic structure of dinoflagellate symbionts in coral recruits differs from that of parental or local adults. Ecology and Evolution, 12(9). Portico. https://doi.org/10.1002/ece3.9312
Methods
Coffroth, M. A., Lasker, H. R., Diamond, M. E., Bruenn, J. A., & Bermingham, E. (1992). DNA fingerprints of a gorgonian coral: a method for detecting clonal structure in a vegetative species. Marine Biology, 114(2), 317–325. doi:10.1007/bf00349534
Methods
Correa, A. M. S., Brandt, M. E., Smith, T. B., Thornhill, D. J., & Baker, A. C. (2009). Symbiodinium associations with diseased and healthy scleractinian corals. Coral Reefs, 28(2), 437–448. https://doi.org/10.1007/s00338-008-0464-6
Methods
Santos, S. R., Gutierrez-Rodriguez, C., & Coffroth, M. A. (2003). Phylogenetic identification of symbiotic dinoflagellates via length heteroplasmy in Domain V of chloroplast Large Subunit (cp23S)-Ribosomal DNA Sequences. Marine Biotechnology, 5(2), 130–140. doi:10.1007/s10126-002-0076-z

Coffroth, M. A., Miller, M. W., Sheets, D. (2023) Breviolum symbiont CP23S genotypes in Orbicella faveolata recruits from 2009-2011 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.882097.1 [access date]

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