Dataset: Nutrients (phosphate and nitrite+nitrate) from daily sampling at the Santa Monica Pier (SMP), Santa Monica Bay, CA from 2018 to 2019

This dataset has not been validatedData not availableVersion 1 (2022-11-17)Dataset Type:Other Field Results

Principal Investigator: David Caron (University of Southern California)

Scientist: Sarah K. Hu (University of Southern California)

Scientist: Gerid A. Ollison (University of Southern California)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Project: Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series (SPOT)


Abstract

This dataset contains nutrients (phosphate and nitrite+nitrate) from daily sampling at the Santa Monica Pier (SMP) from 2018 to 2019. These data were published in Ollison et al (2022). These data were collected as part of a study of high frequency (daily) changes in relative abundance dynamics of the metabolically active protistan community were followed via expressed 18S V4 rRNA genes (RNA) throughout two algal blooms during the spring of 2018 and 2019 in Santa Monica Bay (central Southern C...

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Blooms were targeted in Santa Monica Bay during spring 2018 and 2019 using local meteorological information to anticipate coastal upwelling events (Figure S1, Ollison et al., 2022). Sampling from the Santa Monica Pier (SMP) was conducted daily at 0900 (PST/PDT) from the same location and orientation on the SMP from the 16th through the 30th in April 2018 (15 days), and in 2019 from the 13th April through 6th May (22 days; no sample was collected on the 14th April 2019). Sampling periods are henceforth referred to as 2018 and 2019, respectively.

An RBR Concerto (https://rbr-global.com) was deployed in surface water for 15 minutes at the time of each sample collection to obtain temperature, conductivity, chlorophyll a fluorescence, and dissolved oxygen concentrations (See "Related Dataset" section for CTD data). A 20 µm mesh plankton net was drift towed from the pier (15 min), and samples were examined via light microscopy to identify the dominant planktonic taxa and their relative abundances.

Surface water for 18S rRNA sequencing (see Related Datasets section), nutrient analyses, cell counts, extracted chlorophyll concentrations, and domoic acid concentrations was collected via bucket toss from the pier; an extended funnel was used to gently fill a single acid-washed-3x-rinsed 20 L carboy per established lab protocol (https://www.protocols.io/view/sample-collection-from-the-field-for-downstream-mo-hisb4ee). The carboy was protected from the light and immediately transported approximately 300 meters to the Heal the Bay Aquarium located at the SMP for sample processing.

Samples for nitrite + nitrate and phosphate were measured from 0.02 μm filtered seawater via flow injection analysis on a Quickchem 8500 at the Marine Science Institute Analytical Lab, UCSB (See supplemental files for metadata about these analyses).


Related Datasets

IsRelatedTo

Dataset: Santa Monica Bay Daily CTD
Relationship Description: Data collected as part of targeted sampling of blooms in the Santa Monica Bay during spring 2018 and 2019.
Caron, D., Ollison, G. A., Hu, S. K. (2022) CTD data from daily sampling at the Santa Monica Pier (SMP), Santa Monica Bay, CA from 2018 to 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-11-17 http://lod.bco-dmo.org/id/dataset/883908
IsRelatedTo

Dataset: Santa Monica Bay 18S-V4 sequencing
Relationship Description: Data from the same samples collected as part of targeted sampling of blooms in the Santa Monica Bay during spring 2018 and 2019.
Caron, D., Ollison, G. A., Hu, S. K. (2022) Sampling information and sequence accessions for 18S-V4 sequences from surface water collected at the Santa Monica Pier (SMP) Santa Monica Bay, CA from 2018 to 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-11-17 http://lod.bco-dmo.org/id/dataset/883924

Related Publications

Results

Ollison, G. A., Hu, S. K., Hopper, J. V., Stewart, B. P., Smith, J., Beatty, J. L., Rink, L. K., & Caron, D. A. (2022). Daily dynamics of contrasting spring algal blooms in Santa Monica Bay (central Southern California Bight). Environmental Microbiology. Portico. https://doi.org/10.1111/1462-2920.16137
Methods

Hu, S. (2017). Sample collection from the field for downstream molecular analysis - microbial eukaryote-focused v1. Protocols.io. doi:10.17504/protocols.io.hisb4ee
Methods

Kim, H.-J., Miller, A. J., McGowan, J., & Carter, M. L. (2009). Coastal phytoplankton blooms in the Southern California Bight. Progress in Oceanography, 82(2), 137–147. https://doi.org/10.1016/j.pocean.2009.05.002
Methods

Seubert, E. L., Gellene, A. G., Howard, M. D. A., Connell, P., Ragan, M., Jones, B. H., Runyan, J., & Caron, D. A. (2013). Seasonal and annual dynamics of harmful algae and algal toxins revealed through weekly monitoring at two coastal ocean sites off southern California, USA. Environmental Science and Pollution Research, 20(10), 6878–6895. https://doi.org/10.1007/s11356-012-1420-0