At sea collections
Nine stations were visited during R/V Endeavor EN616 cruise in the northwest Atlantic.
At eight depths, three 10L Niskin samples were taken for discrete measurements of:

1. Chlorophyll, nutrients including nitrate, nitrite, ammonium, phosphate, and silicate
2. Particulate organic carbon (POC) plus particulate organic nitrogen (PON)
3. Particulate inorganic carbon (PIC)
4. Biogenic silica
5. Birefringence counts of coccolithophores (done ashore)
6. Shipboard Yokogawa Fluid Imaging Technologies FlowCam imaging cytometer, in order to enumerate the major microalgal classes and estimate the particle size distribution function

Measurements 1 to 4 are part of BCO-DMO dataset 837074 (See https://www.bco-dmo.org/dataset/837074, and the Related Datasets section below).
Measurement 5 of birefringence counts data is BCO-DMO dataset 887863 (See https://www.bco-dmo.org/dataset/887863, and the Related Datasets section below).
Measurement 6 is flow cytometer data presented in this dataset.

FlowCAM enumeration of various phytoplankton classes
A Yokogowa FlowCAM imaging cytometer was used to enumerate different classes of phytoplankton. The instrument was keyed on particle backscattering and fluorescence properties. Samples were first filtered through 100um Nitex mesh to make sure the 100um diameter flow chamber did not clog. The instrument was run with a 10X objective in order to reliably count particles bigger than 4-5um diameter. Samples were processed according to Poulton and Martin (2010).

Concentrations (per mL), percent contribution with respect to total particles, and biomass are presented. Carbon biomass was determined based on the Menden-Deuer & Lessard (2000) method.

flow_cytometer_en616.csv