Dataset: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.894179.1Version 1 (2023-04-18)Dataset Type:experimental

Principal Investigator: Mary Ann Moran (University of Georgia)

Co-Principal Investigator: Christopher R. Reisch (University of Florida)

Scientist: Catalina Mejia (University of Florida)

Scientist: Lidimarie Trujillo Rodriguez (University of Florida)

Student, Contact: William F. Schroer (University of Georgia)

Data Manager: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Program: Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Project: Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Abstract

High resolution growth screens were used to confirm the phenotype of Ruegeria pomeroyi DSS-3 transporter knockout mutants. Mutants which had demonstrated growth defects on a given substrate as sole carbon source during an initial growth screen were selected and used here. Each mutant was grown on the substrate(s) of interest along side a wildtype analog (pooled-TnSeq library). Growth curves were generated by reading the optical density at 600 nm hourly. The annotations of a transporter's cognate...

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Each column heading provides the treatment condition and replicate. The first 4 letters are substrate abbreviation (provided below) followed by the bacterial identifier. The bacterial identity is either "WT" indicating the wildtype analog (pooled-RBTnSeq library) or four numbers indicating the locus tag of the disrupted gene (e.g. "1234" represents a mutant of "SPO1234").  Finally, the underscore and lowercase letter ("_a",  "_b",  "_c",  "_d") indicates the biological replicate. 

Values are optical density at 600 nm.

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Results
Schroer, W. F., Kepner, H. E., Uchimiya, M., Mejia, C., Rodriguez, L. T., Reisch, C. R., & Moran, M. A. (2023). Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites. https://doi.org/10.1101/2023.01.19.524783
Results
Schroer, W. F., Kepner, H. E., Uchimiya, M., Mejia, C., Rodriguez, L. T., Reisch, C. R., & Moran, M. A. (2023). Functional annotation and importance of marine bacterial transporters of plankton exometabolites. ISME Communications, 3(1). https://doi.org/10.1038/s43705-023-00244-6

Moran, M. A., Reisch, C. R., Mejia, C., Trujillo Rodriguez, L. (2023) High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.894179.1 [access date]

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This dataset is licensed under Creative Commons Attribution 4.0.


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Funded by the U.S. National Science Foundation