Dataset: High resolution screen growth curves

Name: High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)
Published: 2023-04-18
Keywords: RB-TnSeq, gene fitness, transporters, metabolism, arrayed mutant library, genetic screen, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.894179.1

Temporal Extent: 2021-09 - 2022-06

Project:

Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites (C-CoMP Marine Bacterial Transporters)

Program:

Center for Chemical Currencies of a Microbial Planet (C-CoMP)

Principal Investigator:

Mary Ann Moran (University of Georgia, UGA)

Co-Principal Investigator:

Christopher R. Reisch (University of Florida, UF)

Scientist:

Catalina Mejia (University of Florida, UF)

Lidimarie Trujillo Rodriguez (University of Florida, UF-SFRC)

Student:

William F. Schroer (University of Georgia, UGA)

Contact:

William F. Schroer (University of Georgia, UGA)

Data Manager:

Laura Gray (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-04-18

Restricted:

Validated:

Yes

Current State:

Final no updates expected

High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)

Abstract:

High resolution growth screens were used to confirm the phenotype of Ruegeria pomeroyi DSS-3 transporter knockout mutants. Mutants which had demonstrated growth defects on a given substrate as sole carbon source during an initial growth screen were selected and used here. Each mutant was grown on the substrate(s) of interest along side a wildtype analog (pooled-TnSeq library). Growth curves were generated by reading the optical density at 600 nm hourly. The annotations of a transporter's cognate substrate was confirmed when the mutant of said transporter demonstrated significant defect relative to the wildtype analog on the substrate of interest.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-04-18	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.894179.1	2023-05-24

Description

Each column heading provides the treatment condition and replicate. The first 4 letters are substrate abbreviation (provided below) followed by the bacterial identifier. The bacterial identity is either "WT" indicating the wildtype analog (pooled-RBTnSeq library) or four numbers indicating the locus tag of the disrupted gene (e.g. "1234" represents a mutant of "SPO1234"). Finally, the underscore and lowercase letter ("_a", "_b", "_c", "_d") indicates the biological replicate.

Values are optical density at 600 nm.

Methods & Sampling

Methods & Sampling

Mutant cultures were pre-grown overnight in ½ YTSS medium with 50 mg ml-1 kanamycin. Screens were performed in L1 minimal medium (Guillard and Hargraves 1993) (dx.doi.org/10.17504/protocols.io.jvccn2w) modified to a salinity of 20 ppt and amended with ammonium (3 mM), kanamycin (50 mg ml-1), and phosphorus as PO43- at 36 mM. Overnight cultures of individual mutants were washed 3 times in substrate free medium. Four replicate 200 µl mutant cultures were prepared by inoculating 2 ml of washed overnight culture into 96 well plates containing 198 ml modified L1 medium and a single substrate at 8 mM carbon. As a positive control, four wells with the same medium were inoculated with washed overnight cultures of the pooled-RB-TnSeq library, used as a proxy for wild-type R. pomeroyi growth but harboring a transposon/kanamycin resistance gene insertion. Cultures were grown at 25oC in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) shaking at 425 rpm for 68-72 h. OD600 readings were collected once each hour.

Data Processing Description

Data Processing Notes from Submitter:

The data were imported into Excel and compiled on a single sheet. Values are OD600, they have not been pathlength corrected, they have not been blank corrected. We recommend blanking each sample to its first time point.

BCO-DMO Processing Notes:

Replaced spaces and special characters in column header names with underscores ("_")

Data Files

File
combinedgrowthcurves_bco_dmo3_23_2.csv (Comma Separated Values (.csv), 35.34 KB) MD5:66bab205ed3cf81f5e5a42416fc32b5f Primary data file for dataset ID 894179. File processed with laminar pipeline "894179_v1_high_resolution_screen_growth_curves" at path 894179/1/data/combinedgrowthcurves_bco_dmo3_23_2.csv

More Information about this dataset

Funding Sources

Funding Source	Award Number
Simons Foundation	542391
NSF Division of Ocean Sciences	OCE-2019589

Deployments

None available yet

Instruments

plate reader

Supplied Name: Synergy H1 plate reader (BioTek, Winooski, VT, USA)

Supplied Description:

Cultures were grown at 25oC in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) shaking at 425 rpm for 68-72 h. OD600 readings were collected once each hour.

Instrument Type

Generic Name: plate reader

Generic Description:

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
time_hours	Sample time	hours	time_sample
thymWT_a	Thymidine wild type biological replicate a	units	replicate
thymWT_b	Thymidine wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thymWT_c	Thymidine wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thymWT_d	Thymidine wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0378_a	Thymidine locas tag 0378 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0378_b	Thymidine locas tag 0378 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0378_c	Thymidine locas tag 0378 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0378_d	Thymidine locas tag 0378 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0379_a	Thymidine locas tag 0379 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0379_b	Thymidine locas tag 0379 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0379_c	Thymidine locas tag 0379 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
thym0379_d	Thymidine locas tag 0379 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cholWT_a	Choline wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cholWT_b	Choline wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cholWT_c	Choline wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cholWT_d	Choline wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
chol1087_a	Choline locas tag 1087 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
chol1087_b	Choline locas tag 1087 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
chol1087_c	Choline locas tag 1087 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
chol1087_d	Choline locas tag 1087 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSPWT_a	DMSP wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSPWT_b	DMSP wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSPWT_c	DMSP wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSPWT_d	DMSP wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
citrWT_a	Citrate wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
citrWT_b	Citrate wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
citrWT_c	Citrate wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
citrWT_d	Citrate wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPSWT_a	DHPS wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPSWT_b	DHPS wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPSWT_c	DHPS wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPSWT_d	DHPS wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glosWT_a	Glucose wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glosWT_b	Glucose wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glosWT_c	Glucose wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glosWT_d	Glucose wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xyloWT_a	Xylose wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xyloWT_b	Xylose wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xyloWT_c	Xylose wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xyloWT_d	Xylose wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taurWT_a	Taurine wild type biological replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taurWT_b	Taurine wild type biological replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taurWT_c	Taurine wild type biological replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taurWT_d	Taurine wild type biological replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSP3186_a	DMSP locas tag 3186 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSP3186_b	DMSP locas tag 3186 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSP3186_c	DMSP locas tag 3186 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DMSP3186_d	DMSP locas tag 3186 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xylo0863_a	Xylose 0863 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xylo0863_b	Xylose 0863 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xylo0863_c	Xylose 0863 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
xylo0863_d	Xylose 0863 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPS0591_a	DHPS locas tag 0591 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPS0591_b	DHPS locas tag 0591 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPS0591_c	DHPS locas tag 0591 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
DHPS0591_d	DHPS locas tag 0591 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glos0863_a	Glucose locas tag 0863 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glos0863_b	Glucose locas tag 0863 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glos0863_c	Glucose locas tag 0863 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glos0863_d	Glucose locas tag 0863 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0676_a	Taurine locas tag 0676 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0676_b	Taurine locas tag 0676 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0676_c	Taurine locas tag 0676 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0676_d	Taurine locas tag 0676 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0674_a	Taurine locas tag 0674 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0674_b	Taurine locas tag 0674 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0674_c	Taurine locas tag 0674 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
taur0674_d	Taurine locas tag 0674 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcNWT_a	GlcNAc biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcNWT_b	GLcNAc biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcNWT_c	GLcNAc biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcNWT_d	GLcNAc biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcN1839_a	GLcNAc locas tag 1839 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcN1839_b	GLcNAc locas tag 1839 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcN1839_c	GLcNAc locas tag 1839 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glcN1839_d	GLcNAc locas tag 1839 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sperWT_a	Spermidine biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sperWT_b	Spermidine biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sperWT_c	Spermidine biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sperWT_d	Spermidine biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cadaWT_a	Cadaverine biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cadaWT_b	Cadaverine biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cadaWT_c	Cadaverine biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cadaWT_d	Cadaverine biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putrWT_a	Putresceine biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putrWT_b	Putresceine biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putrWT_c	Putresceine biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putrWT_d	Putresceine biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ectoWT_a	Ectoine biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ectoWT_b	Ectoine biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ectoWT_c	Ectoine biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ectoWT_d	Ectoine biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sper3469_a	Spermidine locas tag 3469 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sper3469_b	Spermidine locas tag 3469 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sper3469_c	Spermidine locas tag 3469 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
sper3469_d	Spermidine locas tag 3469 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cada3469_a	Cadaverine locas tag 3469 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cada3469_b	Cadaverine locas tag 3469 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cada3469_c	Cadaverine locas tag 3469 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cada3469_d	Cadaverine locas tag 3469 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putr3469_a	Putresceine locas tag 3469 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putr3469_b	Putresceine locas tag 3469 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putr3469_c	Putresceine locas tag 3469 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
putr3469_d	Putresceine locas tag 3469 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ecto1147_a	Ectoine locas tag 1147 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ecto1147_b	Ectoine locas tag 1147 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ecto1147_c	Ectoine locas tag 1147 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
ecto1147_d	Ectoine locas tag 1147 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
malaWT_a	Malate biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
malaWT_b	Malate biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
malaWT_c	Malate biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
malaWT_d	Malate biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succWT_a	Succinate biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succWT_b	Succinate biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succWT_c	Succinate biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succWT_d	Succinate biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumrWT_a	Fumarate biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumrWT_b	Fumarate biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumrWT_c	Fumarate biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumrWT_d	Fumarate biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2626_a	Malate locus tag 2626 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2626_b	Malate locus tag 2626 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2626_c	Malate locus tag 2626 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2626_d	Malate locus tag 2626 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2628_a	Malate locus tag 2628 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2628_b	Malate locus tag 2628 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2628_c	Malate locus tag 2628 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2628_d	Malate locus tag 2628 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2630_a	Malate locus tag 2630 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2630_b	Malate locus tag 2630 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2630_c	Malate locus tag 2630 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
mala2630_d	Malate locus tag 2630 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2626_a	Succinate locus tag 2626 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2626_b	Succinate locus tag 2626 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2626_c	Succinate locus tag 2626 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2626_d	Succinate locus tag 2626 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2628_a	Succinate locus tag 2628 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2628_b	Succinate locus tag 2628 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2628_c	Succinate locus tag 2628 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2628_d	Succinate locus tag 2628 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2630_a	Succinate locus tag 2630 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2630_b	Succinate locus tag 2630 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2630_c	Succinate locus tag 2630 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
succ2630_d	Succinate locus tag 2630 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2626_a	Fumarate locus tag 2626 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2626_b	Fumarate locus tag 2626 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2626_c	Fumarate locus tag 2626 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2626_d	Fumarate locus tag 2626 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2628_a	Fumarate locus tag 2628 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2628_b	Fumarate locus tag 2628 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2628_c	Fumarate locus tag 2628 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2628_d	Fumarate locus tag 2628 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2630_a	Fumarate locus tag 2630 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2630_b	Fumarate locus tag 2630 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2630_c	Fumarate locus tag 2630 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
fumr2630_d	Fumarate locus tag 2630 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cystWT_a	Cysteate biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cystWT_b	Cysteate biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cystWT_c	Cysteate biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cystWT_d	Cysteate biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glycWT_a	Glycerol biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glycWT_b	Glycerol biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glycWT_c	Glycerol biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glycWT_d	Glycerol biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3040_a	Cysteate locus tag 3040 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3040_b	Cysteate locus tag 3040 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3040_c	Cysteate locus tag 3040 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3040_d	Cysteate locus tag 3040 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glyc0608_a	Glycerol locus tag 0608 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glyc0608_b	Glycerol locus tag 0608 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glyc0608_c	Glycerol locus tag 0608 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
glyc0608_d	Glycerol locus tag 0608 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3041_a	Cysteate locus tag 3041 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3041_b	Cysteate locus tag 3041 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3041_c	Cysteate locus tag 3041 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
cyst3041_d	Cysteate locus tag 3041 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
isetWT_a	Isethionate biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
isetWT_b	Isethionate biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
isetWT_c	Isethionate biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
isetWT_d	Isethionate biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2357_a	Isethionate locus tag 2357 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2357_b	Isethionate locus tag 2357 repilicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2357_c	Isethionate locus tag 2357 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2357_d	Isethionate locus tag 2357 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2358_a	Isethionate locus tag 2358 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2358_b	Isethionate locus tag 2358 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2358_c	Isethionate locus tag 2358 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
iset2358_d	Isethionate locus tag 2358 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butrWT_a	3-Hydroxybutyrate biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butrWT_b	3-Hydroxybutyrate biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butrWT_c	3-Hydroxybutyrate biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butrWT_d	3-Hydroxybutyrate biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carnWT_a	Carnitine biological wild type replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carnWT_b	Carnitine biological wild type replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carnWT_c	Carnitine biological wild type replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carnWT_d	Carnitine biological wild type replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537A_a	3-Hydroxybutyrate locus tag 537A replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537A_b	3-Hydroxybutyrate locus tag 537A replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537A_c	3-Hydroxybutyrate locus tag 537A replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537A_d	3-Hydroxybutyrate locus tag 537A replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537B_a	3-Hydroxybutyrate locus tag 537B replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537B_b	3-Hydroxybutyrate locus tag 537B replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537B_c	3-Hydroxybutyrate locus tag 537B replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
butr537B_d	3-Hydroxybutyrate locus tag 537B replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2995_a	Carnitine locus tag 2995 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2995_b	Carnitine locus tag 2995 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2995_c	Carnitine locus tag 2995 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2995_d	Carnitine locus tag 2995 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2996_a	Carnitine locus tag 2996 replicate a	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2996_b	Carnitine locus tag 2996 replicate b	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2996_c	Carnitine locus tag 2996 replicate c	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate
carn2996_d	Carnitine locus tag 2996 replicate d	Number of sequence counts (barcodes) that mapped to each gene in the treatment.	replicate

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Dataset: High resolution screen growth curves