Dataset: Element quotas of individual phytoplankton cells from samples collected on the US GEOTRACES Arctic cruise GN01 (HLY1502) on USCGC Healy in August-October 2015

Final no updates expectedDOI: 10.26008/1912/bco-dmo.904895.1Version 1 (2023-09-05)Dataset Type:Cruise Results

Principal Investigator: Benjamin Twining (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: U.S. GEOTRACES (U.S. GEOTRACES)

Project: U.S. Arctic GEOTRACES Study (GN01) (U.S. GEOTRACES Arctic)

Project: GEOTRACES Arctic Section: Collaborative Research: Biogeochemical cycling of particulate trace elements in the western Arctic basin (Arctic GEOTRACES bottle particles)


Abstract

Individual phytoplankton cells were collected on the US GEOTRACES Arctic cruise GN01 (HLY1502) on USCGC Healy in August-October 2015. The elemental (Si, P, S, Mn, Fe, Co, Ni, Cu, Zn) content of each cell was measured with synchrotron x-ray fluorescence (SXRF). Carbon was calculated from biovolume. Data can be used to assess biogenic particulate metal fraction, as well as changes in the accumulation of these elements across environmental gradients. Data are part of the larger international GEOTRA...

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Single-cell SXRF samples were collected from the surface mixed layer using the GEOTRACES rosette. Whole water samples were preserved with 0.25% trace-metal clean buffered glutaraldehyde (Twining et al., 2003) and centrifuged onto Au or SiN TEM windows. Grids were briefly rinsed with a drop of ultrapure water and dried in a Class-100 cabinet. SXRF analysis was performed using the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory) following the protocols of Twining et al. (2011). Each cell was raster scanned with a focused 10 keV x-ray beam with a diameter of approximately 0.5 micrometers (µm).


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Results

Bundy, R. M., Tagliabue, A., Hawco, N. J., Morton, P. L., Twining, B. S., Hatta, M., … Saito, M. A. (2020). Elevated sources of cobalt in the Arctic Ocean. Biogeosciences, 17(19), 4745–4767. doi:10.5194/bg-17-4745-2020
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Jensen, L. T., Wyatt, N. J., Twining, B. S., Rauschenberg, S., Landing, W. M., Sherrell, R. M., & Fitzsimmons, J. N. (2019). Biogeochemical Cycling of Dissolved Zinc in the Western Arctic (Arctic GEOTRACES GN01). Global Biogeochemical Cycles, 33(3), 343–369. doi:10.1029/2018gb005975
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Sofen, L. E., O. A. Antipova, K. N. Buck, S. Caprara, L. Chacho, R. J. Johnson, G. Kim, P. Morton, D. C. Ohnemus, S. Rauschenberg, P. N. Sedwick, A. Tagliabue, and B. S. Twining. (2023). Authigenic iron is a significant component of oceanic labile particulate iron inventories. Global Biogeochemical Cycles In revision.
Methods

Hillebrand, H., Dürselen, C.-D., Kirschtel, D., Pollingher, U., & Zohary, T. (1999). Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35(2), 403–424. doi:10.1046/j.1529-8817.1999.3520403.x
Methods

Menden-Deuer, S., & Lessard, E. J. (2000). Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography, 45(3), 569–579. doi:10.4319/lo.2000.45.3.0569