Dataset: Size Fractionated Chlorophyll Dataset

This dataset provides size-fractionated (SF) chlorophyll measurements taken across the subtropical front east of New Zealand in October and November 2018. The dataset provides depth-resolved measurements of SF chlorophyll for 0.2 µm, 2 µm and 20 µm sized cells for locations in the subtropical and subantarctic-influenced locations of the subtropical front. Samples for size fractionated chlorophyll a (SF Chla) were analyzed by first gravity filtering a sample (250 mL) through a 20 µm 47-mm polycarbonate filter, then with low vacuum pressure, filtering sequentially through a 2 µm and a 0.2 µm filter. Filters were then folded, placed in 1.5 mL cryovials and frozen (-80 °C) until analysis (Gutiérrez‐Rodríguez et al., 2020). Chla and acidified phaeopigment-a concentrations were measured (within 3 months) using ice-cold 90% acetone extraction by spectrofluorometric methods (APHA 10200 H) on a Varian Cary Eclipse fluorescence spectrophotometer (Rice et al., 2012). In addition to serving as the chlorophyll measurements for size specific C:Chl ratios, size fractionated chlorophyll measurements are crucial in estimating chlorophyll concentrations for various sized autotroph cells. The summed SF chlorophyll is also provided in this dataset as comparison to total chlorophyll sampled in this region. Andrés Gutierrez Rodriguez and his lab were responsible for collection and analysis of this dataset.