Dataset: Size Fractionated Chlorophyll Measurements from R/V Tangaroa TAN1810 in the Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand) from October to November 2018 (Salp Food Web Ecology project)

This dataset has not been validatedPreliminary and in progressVersion 1 (2023-08-01)Dataset Type:Cruise Results

Principal Investigator: Andres Gutierrez Rodriguez (New Zealand National Institute of Water and Atmospheric Research)

Co-Principal Investigator: Michael R. Stukel (Florida State University)

Co-Principal Investigator: Natalia Yingling (Florida State University)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Quantifying trophic roles and food web ecology of salp blooms of the Chatham Rise (Salp Food Web Ecology)


Abstract

This dataset provides size-fractionated (SF) chlorophyll measurements taken across the subtropical front east of New Zealand in October and November 2018. The dataset provides depth-resolved measurements of SF chlorophyll for 0.2 µm, 2 µm and 20 µm sized cells for locations in the subtropical and subantarctic-influenced locations of the subtropical front. Samples for size fractionated chlorophyll a (SF Chla) were analyzed by first gravity filtering a sample (250 mL) through a 20 µm 47-mm polycar...

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Field Collection

Data was collected in the Chatham Rise section of the Southern Ocean, located east of Aotearoa New Zealand, as part of the SALPOOP (‘Salp Particle expOrt and Ocean Production’) voyage during October to November 2018. ). We conducted five Lagrangian experiments (hereafter referred to as “cycles”) that lasted four to eight days (Décima et al., 2023). There were three cycles that were sampled in SA waters (1, 2 and 5) and two cycles in ST waters (3 and 4) while salps were only present in three cycles (1, 2 and 4). Six depths were chosen to span the euphotic zone (based on chlorophyll fluorescence measured during the conductivity-temperature-depth (CTD) downcast profiles).

Size Fractionated Chlorophyll a Measurements

Samples for size fractionated chlorophyll a (SF Chla) were analyzed by first gravity filtering a sample (250 mL) through a 20 µm 47-mm polycarbonate filter, then with low vacuum pressure, filtering sequentially through a 2 µm and a 0.2 µm filter. Filters were then folded, placed in 1.5 mL cryovials and frozen (-80 °C) until analysis (Gutiérrez‐Rodríguez et al., 2020). Chla and acidified phaeopigment-a concentrations were measured (within 3 months) using ice-cold 90% acetone extraction by spectrofluorometric methods (APHA 10200 H) on a Varian Cary Eclipse fluorescence spectrophotometer (Rice et al., 2012).


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Results

Gutiérrez-Rodríguez, A., Latasa, M., Selph, K. E., Safi, K., Yingling, N., Lopes dos Santos, A., Kelly, T. B., Gorbunov, M. Y., Decima, M., Stukel, M. R., and Nodder, S. D. (In Preparation). Phytoplankton taxon-specific production and microzooplankton consumption in subtropical and subantarctic waters of contrasting productivity
Results

Yingling, N., Selph, K. E., Décima, M., Safi, K., Gutiérrez-Rodríguez, A., Fender, C. K., & Stukel, M. R. (In Preparation). Autotrophic Plankton Size Spectra, Biomass, Abundance, and Community Composition in the Subtropical Convergence Zone in the Southern Ocean. Deep Sea Res. Part I.
Methods

Gutiérrez‐Rodríguez, A., Safi, K., Fernández, D., Forcén‐Vázquez, A., Gourvil, P., Hoffmann, L., Pinkerton, M., Sutton, P., & Nodder, S. D. (2020). Decoupling Between Phytoplankton Growth and Microzooplankton Grazing Enhances Productivity in Subantarctic Waters on Campbell Plateau, Southeast of New Zealand. Journal of Geophysical Research: Oceans, 125(2). Portico. https://doi.org/10.1029/2019jc015550
Methods

Standard Methods for the Examination of Water and Wastewater. ALPHA-AWWA-WEF. Standard Methods Online. https://www.standardmethods.org/