Dataset: A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.906617.1Version 1 (2023-09-27)Dataset Type:Other Field Results

Principal Investigator: Adrienne M.S. Correa (Rice University)

Principal Investigator: Rebecca Vega Thurber (Oregon State University)

Co-Principal Investigator: Andrew Thurber (Oregon State University)

Scientist: Lauren I. Howe-Kerr (Rice University)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)

Abstract

These data are from a study that used amplicon sequencing of the viral major capsid protein (mcp) gene of positive-sense single-stranded RNA viruses known to infect symbiotic dinoflagellates ('dinoRNAVs') to analyze their dynamics in the reef-building coral, Porites lobata. The investigators repeatedly sampled 54 colonies across three environmentally distinct reef zones (fringing reef, back reef, and forereef) around the island of Moorea, French Polynesia over a three-year period and spanning a ...

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Cite Dataset

This dataset is comprised of three components:

(1) temperature data measured at each reef site - available in the csv data file "906617_v1_temperature_moorea_reefs.csv"

(2) images of Porites lobata colonies taken at each site - available in the PDF "POR colony health images.pdf"

(3) dinoRNAV mcp gene amplicon libraries and Symbiodiniaceae LSU gene amplicon libraries - available in the National Center for Biotechnology Information Sequence Read Archive under accession numbers PRJNA928208 and PRJNA930706, respectively. Parameters used to analyze the viral data are described in Zenodo at https://zenodo.org/record/7552892#.Y_PAa-zMK3I (doi: 10.5281/zenodo.7552892)

Results of this study have been published in Howe-Kerr et al., 2023. (doi: 10.1038/s43705-023-00227-7).

Related Datasets

IsRelatedTo
Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA930706
Rice University. Symbiodiniaceae, Porites lobata Symbiodiniaceae LSU amplicon sequencing 2018-2020 Moorea, French Polynesia. 2023/02. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA930706. NCBI:BioProject: PRJNA930706.
IsRelatedTo
Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA928208
Rice University. dinoRNA virus, Porites lobata Symbiodiniaceae infecting RNA viruses. 2023/01. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA928208. NCBI:BioProject: PRJNA928208.

Related Publications

Results
Howe-Kerr, L. I., Grupstra, C. G. B., Rabbitt, K. M., Conetta, D., Coy, S. R., Klinges, J. G., Maher, R. L., McConnell, K. M., Meiling, S. S., Messyasz, A., Schmeltzer, E. R., Seabrook, S., Sims, J. A., Veglia, A. J., Thurber, A. R., Thurber, R. L. V., & Correa, A. M. S. (2023). Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape. ISME Communications, 3(1). https://doi.org/10.1038/s43705-023-00227-7
Methods
Frøslev, T. G., Kjøller, R., Bruun, H. H., Ejrnæs, R., Brunbjerg, A. K., Pietroni, C., & Hansen, A. J. (2017). Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates. Nature Communications, 8(1). https://doi.org/10.1038/s41467-017-01312-x
Methods
Grupstra, C. G. B., Howe-Kerr, L. I., Veglia, A. J., Bryant, R. L., Coy, S. R., Blackwelder, P. L., & Correa, A. M. S. (2022). Thermal stress triggers productive viral infection of a key coral reef symbiont. The ISME Journal, 16(5), 1430–1441. https://doi.org/10.1038/s41396-022-01194-y
Methods
Howe-Kerr, L., Veglia, A., &amp; Correa, A. (2023). vAMPirus Analysis - Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape (Version 1.0.0). Zenodo. https://doi.org/10.5281/ZENODO.7552892
Methods
Montalvo-Proaño, J., Buerger, P., Weynberg, K. D., & van Oppen, M. J. H. (2017). A PCR-Based Assay Targeting the Major Capsid Protein Gene of a Dinorna-Like ssRNA Virus That Infects Coral Photosymbionts. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.01665

Correa, A. M., Vega Thurber, R., Thurber, A., Howe-Kerr, L. I. (2023) A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral &lt;i&gt;Porites c.f. lobata&lt;/i&gt; at three reef zones around Moorea, French Polynesia. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.906617.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation