Dataset: Fatty acid measurements – field samples and lab eggs

Name: Fatty acid measurements for various marine samples collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022 as well as of red drum eggs spawned from captive adults from 2020 to 2022
Published: 2023-09-19
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.908698.1

Spatial Extent: N:27.9362 E:-97.0218 S:27.6469 W:-97.3150222

Temporal Extent: 2020-07-01 - 2023-03-21

Project:

Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons (Egg Boon Food Webs)

Principal Investigator:

Lee A. Fuiman (University of Texas - Marine Science Institute, UTMSI)

Co-Principal Investigator:

Parvathi Nair (University of Texas - Marine Science Institute, UTMSI)

Contact:

Lee A. Fuiman (University of Texas - Marine Science Institute, UTMSI)

BCO-DMO Data Manager:

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-09-19

Restricted:

Release Date:

2024-08-01

Validated:

Yes

Current State:

Final no updates expected

Fatty acid measurements for various marine samples collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022 as well as of red drum eggs spawned from captive adults from 2020 to 2022

Abstract:

Fatty acid measurements for various marine samples collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022, as well as eggs and food sources from the laboratory and commercial sources from 2020 to 2022.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2023-09-19	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.908698.1	2024-01-17

Methods & Sampling

Location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222); MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777); TPWD: Texas Parks and Wildlife Department, Corpus Christi, TX, United States (lat. 27.646877, lon. -97.3150222))

Methods and Sampling:

A sampling of likely egg consumers, egg non-consumers, pelagic plankton, and benthic sediments began in July 2020 and ended in March 2022. Samples were collected from two field sites, one in the vicinity of the Red Drum spawning aggregation (Aransas Pass Inlet) and the other outside the extent of the egg boon (Mud Island in Aransas Bay) by deploying a 500-micron (µm) mesh plankton net. Animals in the plankton net were sorted immediately after collection and transferred to holding tanks to evacuate their guts for 3-4 hours. Each sample was then rinsed twice in distilled water and frozen at -80°C for subsequent fatty acid analysis.

Basal resources were collected by pumping seawater from the Aransas Pass Channel adjacent to the University of Texas Marine Science Institute’s Fisheries and Mariculture Laboratory and filtering the seawater through sieves of three different mesh sizes to obtain three size fractions of plankton samples (10-60 µm, 100-200 µm, and 200-500 µm). Bottom sediment was collected using a grab sampler and passed through a 60‑µm sieve to obtain benthic particulate organic matter. Samples of jellyfish and small fishes were taken with dip nets, cast nets, or seines and processed as described above for analysis.

Fatty acid profiles were measured for samples of fish eggs, marine animals, and basal resources collected from the field as well as eggs and marine animals used in laboratory experiments. The time between collection and analysis ranged from 2 months to a year.

For fatty acid analysis, each sample was lyophilized, homogenized, and weighed. Lipids were cold extracted from a known subsample (by weight) of the homogenized and lyophilized tissue using chloroform/methanol/water (8:4:3 v/v) following the method of Folch et al. (1957. Journal of Biological Chemistry 226:497–507) with 0.01% (w/v) butylated hydroxytoluene as an antioxidant. For small taxa or taxa with low lipid content (e.g., ctenophores), several individuals were pooled into one sample. Fatty acid methyl esters (FAME) were prepared by transesterification with 14% boron trifluoride in methanol (Morrison and Smith 1964. Journal of Lipid Research 5:600–608) following saponification of the total lipid using 0.5M potassium hydroxide in methanol. FAMEs were measured by gas chromatography with flame ionization detector following procedures described by Faulk and Holt (2005. Aquaculture 249:231–243). Measurements were expressed in terms of concentration (mg per g dry weight) and composition (% of total fatty acids). Fatty acids were identified by comparison with commercial standards.

Known Issues:
Quality control procedure
Principal components analysis for each taxon was performed on the mg g-1 dw and percent total fatty acids data, separately. Individual samples for which the score on the first or second principal component axis were greater than 4 standard deviations from the taxon mean were removed from the data set.

A primary check value was assigned as follows:

1 Perfectly fine

2 Data not evaluated because of too few data points for principal components analysis

The quantification limit for individual fatty acids is 0.013 mg g-1 dry weight

Measured values of any fatty acid less than 0.013 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%.

FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0727222);

MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).

Data Processing Description

Identified peaks from the gas chromatograms were quantified using commercial FAME standards, such as Supelco 37 Component FAME mix, polyunsaturated fatty acid mix no. 3 (PUFA-3), and bacterial acid methyl ester mix (BAME).

BCO-DMO Processing

BCO-DMO Data Manager Processing Notes:
* Sheet 1 of file "Field FA data 2020-2022.xlsx" (submitted jun 22, 2023 by email) was imported into the BCO-DMO data system with values "NA" as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Dates converted to ISO 8601 format
* one instance of FAMl with lowercase l changed to FAML for consistency with the rest of the dataset and related datasets.
* lat lon columns added from locations in metadata
* updated longitude for site TPWD (update provided by submitter) lon. -97.3150222

Data Files

File
908698_v1_fatty_acid_field-animals_lab-eggs.csv (Comma Separated Values (.csv), 555.07 KB) MD5:4d0943ecada08bbbb1fcd4fa2d23f6d5 Primary data table for dataset 908698 version 1

Supplemental Files

File

Species List and Taxonomic Identifiers
filename: 908698_species_list.csv

(Comma Separated Values (.csv), 6.27 KB)
MD5:532bdea38c345436d6a5a2b1b198a080

Unique species list for this dataset with the matched taxonomic identifiers. Match performed using the World Register of Marine Species (WoRMS) taxa match tool on 2023-12-05. All exact matches to known taxonomic names. Taxon_in_dataset "Salpa" for "salps" was matched to family taxon Salpidae.

columns:
Taxon_in_dataset, Taxon(category) name in the dataset which may contain lifestage terms as well as taxon name (e.g. "Sciaenops ocellatus lab eggs").
ScientificName, Taxon name matched to the "Taxon_in_dataset" which does not include lifestage terms or sp.
Common_name_in_dataset, Common name in the dataset for the category
AphiaID, Taxonomic identifier (AphiaID) for the taxonomic name used in the dataset (see World Register of Marine Species)
LSID, Lifesciences Identifier (LSID) for the taxonomic name used in the dataset.
Taxon status, Status of the name used in the dataset (denotes if the currently accepted name or a currently unaccepted synonym as of the date 2023-12-05)
ScientificName_accepted, The currently accepted name for the taxonomic name used in the dataset.
AphiaID_accepted, The taxonomic identifier (AphiaID)  for the currently accepted name (see World Register of Marine Species) 

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-2023618

Deployments

None available yet

Instruments

Gas Chromatograph

Supplied Name: Shimadzu GC-2014 gas chromatograph with a flame ionization detector

Supplied Description:

Instrument Type

Generic Name: Gas Chromatograph

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/

Generic Description:

Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Taxon	Taxonomic grouping of sample	unitless	taxon
Common_name	Common name of sample	unitless	common_name
Sample_ID	Sample identifier for a taxon on a sampling date	unitless	sample
Year	Year sample was collected	unitless	year
Month	Month sample was collected	unitless	month
Date_collected	Date sample was collected. ISO 8601 format	unitless	date
Date_analyzed	Date sample was collected. ISO 8601 format	unitless	date
Classification	Taxonomic group to which sample belongs	unitless	animal_group
Notes	Notes about sample	unitless	comment
Site	Location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute; MI: Mud Island in Aransas Bay, TX, United States; TPWD: Texas Parks and Wildlife Department, Corpus Christi, TX, United States.	unitless	site
lat	Site latitude	decimal degrees	lat
lon	Site longitude	decimal degrees	lon
Length	Bell diameter in centimeter for cnidarians; total length in centimeter for fish; carapace length in centimeter for crabs	centimeters (cm)	length
Primary_check	Primary QC check	unitless	q_flag
C14_pt_0_mg	measured value of fatty acid 14:0 ; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C15_pt_0_mg	measured value of fatty acid 15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C16_pt_0_mg	measured value of fatty acid 16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C16_pt_1n7_mg	measured value of fatty acid 16:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C16_pt_2n4_mg	measured value of fatty acid 16:2n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C17_pt_0_mg	measured value of fatty acid 17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C16_pt_3n4_mg	measured value of fatty acid 16:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C17_pt_1_mg	measured value of fatty acid 17:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_0_mg	measured value of fatty acid 18:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_1n9_mg	measured value of fatty acid 18:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_1n7_mg	measured value of fatty acid 18:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_2n6_mg	measured value of fatty acid 18:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_3n6_mg	measured value of fatty acid 18:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_3n4_mg	measured value of fatty acid 18:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_3n3_mg	measured value of fatty acid 18:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C18_pt_4n3_mg	measured value of fatty acid 18:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_1n9_mg	measured value of fatty acid 20:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_2n6_mg	measured value of fatty acid 20:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_3n6_mg	measured value of fatty acid 20:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_4n6_mg	measured value of fatty acid 20:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_3n3_mg	measured value of fatty acid 20:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_4n3_mg	measured value of fatty acid 20:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_5n3_mg	measured value of fatty acid 20:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C22_pt_1n11_mg	measured value of fatty acid 22:1n-11; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C22_pt_4n6_mg	measured value of fatty acid 22:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C22_pt_5n6_mg	measured value of fatty acid 22:5n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C22_pt_5n3_mg	measured value of fatty acid 22:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C22_pt_6n3_mg	measured value of fatty acid 22:6n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C20_pt_0_mg	measured value of fatty acid 20:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
Ci_15_pt_0_mg	measured value of fatty acid iso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C14_pt_1_mg	measured value of fatty acid 14:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
Ci_17_pt_0_mg	measured value of fatty acid iso-17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
Ca_15_pt_0_mg	measured value of fatty acid anteiso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
Ci_16_pt_0_mg	measured value of fatty acid iso-16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	milligrams per gram dry weight (mg g-1 dw)	no_bcodmo_term
C14_pt_0_pct	measured value of fatty acid 14:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C15_pt_0_pct	measured value of fatty acid 15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C16_pt_0_pct	measured value of fatty acid 16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C16_pt_1n7_pct	measured value of fatty acid 16:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C16_pt_2n4_pct	measured value of fatty acid 16:2n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C17_pt_0_pct	measured value of fatty acid 17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C16_pt_3n4_pct	measured value of fatty acid 16:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C17_pt_1_pct	measured value of fatty acid 17:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_0_pct	measured value of fatty acid 18:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_1n9_pct	measured value of fatty acid 18:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_1n7_pct	measured value of fatty acid 18:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_2n6_pct	measured value of fatty acid 18:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_3n6_pct	measured value of fatty acid 18:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_3n4_pct	measured value of fatty acid 18:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_3n3_pct	measured value of fatty acid 18:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C18_pt_4n3_pct	measured value of fatty acid 18:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_1n9_pct	measured value of fatty acid 20:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_2n6_pct	measured value of fatty acid 20:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_3n6_pct	measured value of fatty acid 20:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_4n6_pct	measured value of fatty acid 20:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_3n3_pct	measured value of fatty acid 20:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_4n3_pct	measured value of fatty acid 20:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_5n3_pct	measured value of fatty acid 20:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C22_pt_1n11_pct	measured value of fatty acid 22:1n-11; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C22_pt_4n6_pct	measured value of fatty acid 22:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C22_pt_5n6_pct	measured value of fatty acid 22:5n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C22_pt_5n3_pct	measured value of fatty acid 22:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C22_pt_6n3_pct	measured value of fatty acid 22:6n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C20_pt_0_pct	measured value of fatty acid 20:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
Ci_15_pt_0_pct	measured value of fatty acid iso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
C14_pt_1_pct	measured value of fatty acid 14:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
Ci_17_pt_0_pct	measured value of fatty acid iso-17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
Ca_15_pt_0_pct	measured value of fatty acid anteiso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term
Ci_16_pt_0_pct	measured value of fatty acid iso-16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%	% total fatty acids	no_bcodmo_term