Dataset: Pigment concentrations determined by HPLC from samples collected on Leg 1 (Seattle, WA to Hilo, HI) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1814) on R/V Roger Revelle from September to October 2018

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.914655.1Version 1 (2023-11-09)Dataset Type:Cruise Results

Principal Investigator: Gregory A. Cutter (Old Dominion University)

Co-Principal Investigator: Karen L. Casciotti (Stanford University)

Co-Principal Investigator: Phoebe J. Lam (University of California-Santa Cruz)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: U.S. GEOTRACES (U.S. GEOTRACES)

Project: US GEOTRACES Pacific Meridional Transect (GP15) (U.S. GEOTRACES PMT)


Abstract

This dataset contains high-performance liquid chromatography (HPLC) pigment data (19But, 19Hex, Allo, β Car, α Car, Chl a, Chl b, Chlc 1,2, Chl c 3, Cphlid, Diadino, Diato, Fuco, Lut, Neo, Peri, Prasino, Viola, Zea) from the U.S. GEOTRACES GP15 Pacific Meridional Transect from Alaska (56°M) to Tahiti (20°S) along 152°W between September 24, 2018 and November 24, 2018. Samples for pigment analysis were collected at 6 depths at every major station (N=23 stations) and filtered onto GF/F filters, wh...

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Pigments were sampled from the shallowest 6 depths (<=150 meters) on every "PigRaTh" cast (including the surface bottle) at all major stations (N=23 stations). Pigments were collected into 2-liter (L) amber bottles, triple-rinsed with sample prior to filling. They were immediately filtered under vacuum through 47-millimeter (mm) GF/F filters. They were folded and placed inside cryovials, labeled with appropriate GEOTRACES numbers, and frozen at -80° Celsius (C). Vials were shipped in LN2 dry shippers to Stanford, and then on dry ice to the HPLC Facility at Oregon State University (OSU) managed by Ricardo Letelier (https://ceoas.oregonstate.edu/ocean-ecology-and-biogeochemistry-facilities).

OSU HPLC facility methods: Our method for pigment analysis is based on the protocol described by Mantoura and Lewellyn (1983) and modified according to Bidigare et al. (1989). Briefly, samples are extracted for up to 48 hours in 100% acetone at -20°C, then analyzed on a Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector. Pigment standards for calibration and response factor calculations are obtained from DHI Group in Denmark.


Related Datasets

IsContinuedBy

Dataset: GP15 HPLC pigments Leg 2
Relationship Description: GP15 was made up of two cruise legs, RR1814 (Leg 1) and RR1815 (Leg 2)
Cutter, G. A., Casciotti, K. L., Lam, P. J. (2023) Pigment concentrations determined by HPLC from samples collected on Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from Oct-Nov 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-11-13 doi:10.26008/1912/bco-dmo.914845.1

Related Publications

Methods

Bidigare, R. R., Schofield, O., & Prézelin, B. B. (1989). Influence of zeaxanthin on quantum yield of photosynthesis of Synechococcus clone WH7803 (DC2). Marine Ecology Progress Series, 56(1/2), 177–188. http://www.jstor.org/stable/24835757
Methods

Jeffrey, S. W., Mantoura, R. F. C., Wright, S. W., International Council of Scientific Unions., & Unesco. (1997). Phytoplankton pigments in oceanography: Guidelines to modern methods. Paris: UNESCO Pub.
Methods

Mantoura, R. F. C., & Llewellyn, C. A. (1983). The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography. Analytica Chimica Acta, 151, 297–314. https://doi.org/10.1016/s0003-2670(00)80092-6