Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
  • BCO-DMO Processing Description
  • Problem Description
• Data Files
• Supplemental Files
• Related Publications
• Parameters
• Instruments
• Deployments
• Project Information
• Program Information
• Funding

Cruises
Data were collected on BATS Validation (BVAL) cruises, from cruise #1 (April 1991) through BATS cruise # 60 (June 2023). Please note that BVAL cruises 4, 25, 43, and 54 were cancelled and hence no reporting. 

Research was conducted  on the R/V Weatherbird II through 2005 and thereafter on the R/V Atlantic Explorer. There were numerous Chief Scientists for these cruises including Rachel Dow, Anthony Michaels, Kjell Gundersen, Rodney Johnson, Paul Lethaby, Mike Lomas, Steven Bell, Gwyn Evans, and Claire Medley. Cruise track information showing CTD station locations and cruise dates for each BVAL cruise conducted between 1991 and 2022 are available in the Supplemental Files section below. 

Water sampling
Full depth water sampling and data collection at the BATS site are achieved with a total of three hydrocasts using a General Oceanics Intelligent Rosette® with an array of 24 12L water bottles and a Sea-Bird Scientific CTD system. Water samples are collected during the upcast with a 1-minute resting period between reaching the sampling depth and triggering the bottle to close.  Bottom measurements/ sampling are achieved within 20 meters from the bottom, as determined using an altimeter.

Water samples are taken right after Rosette® recovery. On any cast, if only a single water bottle is collected to sample all biogeochemical parameters, then gas samples are collected first due to their exposure to air when opened. However, if enough bottles are available, two bottles can be taken for a single depth. Water is usually split between large-volume particulate samples (POCN, HPLC, POP and PSi) and all other small volume samples, including gas samples. When two bottles are taken for a single depth, particulate samples are collected first to prevent settling within the Niskin bottle. Samples are fixed or frozen once all same-sample bottles from one cast have been collected. Particulate samples are filtered as soon as collected.

Nutrients
The BATS nutrient methodology is based on the Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements (Intergovernmental Oceanographic Commission, 1994) which describes the method for the determination of dissolved inorganic macronutrients in seawater: nitrite (NO2 – ), nitrate + nitrite (NO3 – + NO2 – ), orthophosphate (PO4 3 – ) and reactive silicate (Si(OH)4) using Continuous Flow Analysis (CFA). 

While the definition of the dissolved fraction has changed throughout the years that the BATS time series has operated, the pore size used has remained constant in order to create a comparable temporal dataset. While similar studies in oligotrophic ocean regions have opted to forego the use of nutrient filters under the assumption that the particulate nutrient pool is negligible, we continue the use of filters for the sake of continuity. Sample filtration also removes the potential for turbidity-derived uncertainties during analysis, and may aid preservation of frozen samples.

Discrete samples are collected at the Bermuda Atlantic Time-series Study (BATS) site from surface to bottom depths (∼4,200 meters). Sea water is filtered directly from the Niskin spigot using a 0.8 µm membrane to remove particulates. Collected sea water is preserved by freezing until analysis. Replicate samples are taken during each cast to ensure quality control standards are met during analytical and data processes. Dissolved inorganic nutrients are measured using a SEAL AA500 Autoanalyzer by Continuous Flow Analysis (CFA). During this process, a subset of sample is drawn and further split into four different channels driven by a peristaltic pump. The sample stream is segmented with air or nitrogen bubbles throughout the flow path to enhance the mixing of reagents with the sample. The nutrients, NO₂-, nitrate + nitrite (NO₃– + NO2-) , PO₄^3– and Si, are chemically reacted in the separate channels to produce a color change and are measured colorimetrically at different wavelengths using a flow-through colorimeter located at the end of the flow path. The light absorption by the sample-reagent mixture is proportional to the concentration of nutrient in the sample according to the principles of the Beer-Lambert Law. Raw absorbance units are converted into nutrient concentrations according to a linear calibration curve formulated from known standards.

Bacterial enumeration
In addition to the casts for shallow water, mode water, and deep water, a separate cast is deployed for the estimation of bacterial growth rates using 3H-thymidine. Heterotrophic bacteria are expected to grow and assimilate 3H-thymidine into nucleic acid material under incubation conditions.

Three replicate samples from the same depth are used as live tubes for thymidine incorporation, and are incubated for four hours.  Samples used as killed controls (aka kill tubes) are treated with 100 microliters of 100% TCA (trichloroacetic acid) at the beginning of the incubation to halt biological activity. After incubation, 10 microliters (µl) from the live tubes are extracted for Specific Activities measurements and the biological activity in the live tubes is halted by adding 100% TCA. 

All tubes are centrifuged at 14,000 RPM at 4°C for 7 minutes. The supernatant is discarded and DNA is extracted by adding 100% TCA; centrifuging again for 7 minutes at 4°C at 14,000 RPM;  adding 80% ethanol and centrifuging once more for 7 minutes at 4°C at 14,000 RPM. The DNA in the resulting pellet is resuspended in Ultima Gold by vortexing. Samples are stored at room temperature until analysis.

Data processing steps are outlined in the BATS Methods Manual

Primary Data File
- Imported data from source files "bval_bottle_v004.txt" and "bval_bottle_qcmask_004.txt" into the BCO-DMO data system using missing data identifiers 'nd' and '-999'.
- Merged the mask and bottle data files to create one comprehensive file
- Combined the date (yyyymmmdd) and Time (HHMM) columns into single DateTime column in ISO Date 8601 format
- Created columns for Cruise, Cast, and Bottle based on the ID column

Supplemental Files
- Created a cruise metadata table with cruise ID, cruise dates, plus number of casts
- Zipped folder of cruise sampling location maps in PDF format

A full description of the BATS research program (including links to the processed BATS data) is available from the BATS Web site (see above for Project URL/ Project Website links). Any data contributed from selected ancillary projects are listed (linked) in the 'Datasets Collection' section below.  

Collaborative Research: The Bermuda Atlantic Time-series Study: Sustained Biogeochemical, Ecosystem and Ocean Change Observations and Linkages in the North Atlantic (Years 31-35)
Awards OCE-1756105, OCE-1756054, and OCE-1756312)
NSF award abstract
Long-term observations over several decades are a powerful tool for investigating ocean physics, biology, and chemistry, and the response of the oceans to environmental change. The Bermuda Atlantic Time-Series Study, known as BATS, has been running continuously since 1988. The research goals of the BATS program are: (1) to improve our understanding of the time-varying components of the ocean carbon cycle and the cycles of related nutrient elements such as nitrogen, phosphorus, and silicon; and, (2) to identify the relevant physical, chemical and ecosystem properties responsible for this variability. In addition, the BATS program has strong and diverse broader impacts, contributing to the field of ocean sciences by providing high quality ocean observations and data for seagoing scientists and modelers, and a framework through which researchers can conceive and test hypotheses. This award will support the operations of the BATS program for five more years.

The primary BATS research themes are as follows: (1) Quantify the role of ocean-atmosphere coupling and climate variability on air-sea exchange of CO2, and carbon export to the ocean interior; (2) Document trends and the controls on the interannual to decadal scale variability in carbon and nutrient cycles to their coupling in the surface and deep ocean via the Redfield Ratio paradigm; (3) Quantify the response of planktonic community structure and function, and impact on biogeochemical cycles to variability in surface fluxes and dynamical processes; (4) Facilitate development, calibration and validation of next generation oceanographic sensors, tools and technologies; and, (5) Generate a dataset that can be utilized by empiricists, modelers and students. This research integrates ocean physics, chemistry and biology into a framework for understanding oceanic processes and ocean change in the North Atlantic subtropical gyre. The existing 29 years of BATS data provide robust constraints on seasonal and interannual variability, the response of the Sargasso Sea ecosystem to natural climate variability, and signal detection of potential ocean changes. This project would extend the BATS program through years 31-35 to address a series of ten interlinked questions through integrated research approaches and a multitude of collaborative efforts. In addition to the themes above, and embedded into the ten questions and approaches, the BATS team will focus on, for example, coupling of particle production and biogeochemistry; revisiting the complexities of the biological carbon pump; oxygen decline; and changes in the hydrography, physics, ocean carbon cycle and biogeochemistry of the Sargasso Sea. The highest quality data observation and collection will be maintained and used to address these questions. Importantly, a wide range of collaborations at the BATS site, spanning the physical and biogeochemical disciplines, will aid these broad goals. Strong links to community stakeholders, and close collaboration (including methods intercomparisons and personnel exchanges) with the Hawaii Ocean Time-series are proposed. This work will extend the research findings of the project into educational and training opportunities within and beyond the oceanographic community, including training and mentorship of both undergraduate and graduate students.

Please see the BATS Web site (http://bats.bios.edu) for additional information.

List of References (PDF)

The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.

The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.

The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.

The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.

The United States Joint Global Ocean Flux Study was a national component of international JGOFS and an integral part of global climate change research.

The U.S. launched the Joint Global Ocean Flux Study (JGOFS) in the late 1980s to study the ocean carbon cycle. An ambitious goal was set to understand the controls on the concentrations and fluxes of carbon and associated nutrients in the ocean. A new field of ocean biogeochemistry emerged with an emphasis on quality measurements of carbon system parameters and interdisciplinary field studies of the biological, chemical and physical process which control the ocean carbon cycle. As we studied ocean biogeochemistry, we learned that our simple views of carbon uptake and transport were severely limited, and a new "wave" of ocean science was born. U.S. JGOFS has been supported primarily by the U.S. National Science Foundation in collaboration with the National Oceanic and Atmospheric Administration, the National Aeronautics and Space Administration, the Department of Energy and the Office of Naval Research. U.S. JGOFS, ended in 2005 with the conclusion of the Synthesis and Modeling Project (SMP).

Program description text taken from Chapter 1: Introduction from the Global Intercomparability in a Changing Ocean: An International Time-Series Methods Workshop report published following the workshop held November 28-30, 2012 at the Bermuda Institute of Ocean Sciences. The full report is available from the workshop Web site hosted by US OCB: http://www.whoi.edu/website/TS-workshop/home

Decades of research have demonstrated that the ocean varies across a range of time scales, with anthropogenic forcing contributing an added layer of complexity. In a growing effort to distinguish between natural and human-induced earth system variability, sustained ocean time-series measurements have taken on a renewed importance. Shipboard biogeochemical time-series represent one of the most valuable tools scientists have to characterize and quantify ocean carbon fluxes and biogeochemical processes and their links to changing climate (Karl, 2010; Chavez et al., 2011; Church et al., 2013). They provide the oceanographic community with the long, temporally resolved datasets needed to characterize ocean climate, biogeochemistry, and ecosystem change.

The temporal scale of shifts in marine ecosystem variations in response to climate change are on the order of several decades.  The long-term, consistent and comprehensive monitoring programs conducted by time-series sites are essential to understand large-scale atmosphere-ocean interactions that occur on interannual to decadal time scales.  Ocean time-series represent one of the most valuable tools scientists have to characterize and quantify ocean carbon fluxes and biogeochemical processes and their links to changing climate.

Launched in the late 1980s, the US JGOFS (Joint Global Ocean Flux Study; http://usjgofs.whoi.edu) research program initiated two time-series measurement programs at Hawaii and Bermuda (HOT and BATS, respectively) to measure key oceanographic measurements in oligotrophic waters. Begun in 1995 as part of the US JGOFS Synthesis and Modeling Project, the CARIACO Ocean Time-Series (formerly known as the CArbon Retention In A Colored Ocean) Program has studied the relationship between surface primary production, physical forcing variables like the wind, and the settling flux of particulate carbon in the Cariaco Basin.

The objective of these time-series effort is to provide well-sampled seasonal resolution of biogeochemical variability at a limited number of ocean observatories, provide support and background measurements for process-oriented research, as well as test and validate observations for biogeochemical models. Since their creation, the BATS, CARIACO and HOT time-series site data have been available for use by a large community of researchers.
 
Data from those three US funded, ship-based, time-series sites can be accessed at each site directly or by selecting the site name from the Projects section below.