Dataset: TEN Seagrass Soluble Carbohydrates

Name: Thalassia testudinum belowground carbohydrate measurements from experimental plots in the Western Atlantic from Winter 2019 (Tropicalization Seagrass Beds project)
Published: 2024-01-09
Keywords: seagrass, soluble carbohydrates, western atlantic, oceans

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Cite This Dataset

Spatial Extent: N:32.2639 E:-64.8307 S:9.35161 W:-95.171

Subtropical Western Atlantic

Temporal Extent: 2019-02 - 2019-03

Project:

Collaborative Research: The tropicalization of Western Atlantic seagrass beds (Tropicalization Seagrass Beds)

Principal Investigator:

Justin Campbell (Florida International University, FIU)

Contact:

Justin Campbell (Florida International University, FIU)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-01-09

Restricted:

Validated:

Current State:

Preliminary and in progress

Thalassia testudinum belowground carbohydrate measurements from experimental plots in the Western Atlantic from Winter 2019 (Tropicalization Seagrass Beds project)

Abstract:

This dataset includes seagrass belowground carbohydrates (Thalassia testudinum) from 89 experimental plots distributed across 9 sites in the Western Atlantic. Belowground biomass was collected at the end of the winter season in 2019. This material was then dried, ground to a fine powder and analyzed for soluble carbohydrates by the UC Davis plant lab. Sites include: Bocas del Toro, Panama; Lac Bay, Bonaire; Carrie Bow, Belize; Puerto Morelos, Mexico; Andros, Bahamas; Galveston, Texas; Crystal River, Florida; St. Joes, Florida; and Riddell's Bay, Bermuda.

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Methods & Sampling

Sampling details

Small seagrass plots (0.25m2, all comprised of one species, Thalassia testudinum) were established at 9 shallow sites in the western Atlantic. Each plot was assigned to one of two treatments (n=5/treatment): (1) control plot or (2) plots with the canopy fully clipped. Plots were maintained for 12 months, and at the end, seagrass biomass cores (15cm in diameter) were used to harvest belowground plant material in each plot. Belowground biomass (rhizomes only) were separated from all other material, dried to a constant weight in a 60C oven and ground to a fine powder. This belowground material was then sent to the UC Davis plant lab for the analysis of total soluble carbohydrates (glucose, fructose, sucrose) following the protocols of Smith (1969).

Total Glucose for Total Nonstructural Carbohydrate (TNC) and Startch Methods Summary

The dataset shows soluble carbohydrates expressed as a percentage of belowground dry mass. Nonstructural carbohydrates are those that can be accumulated and then readily mobilized in order to be metabolized or translocated to other plant parts. This method quantitatively determines the amount of the total glucose following enzymatic hydrolysis. Total non-structural carbohydrates (TNC) is the sum of total glucose, free fructose and free sucrose. Starch is the total glucose minus the free glucose multiplied by 0.9. The free carbohydrates are determined by a separate analysis. The samples for total glucose are enzymatically hydrolyzed at 55°C with amyloglucosidase for 12 hours and analyzed by HPLC with mass selective detection. The analysis uses a Phenomenex Luna NH2 (250 mm x 4.6 mm) HPLC column at a flow rate of 2.75 mL min-1 acetonitrile:water (78:22).

The method has a detection limit of 0.5% and is reproducible to within 10% (relative).

Smith, Dale. Removing and Analyzing Total Nonstructural Carbohydrates from Plant Tissue. Wisconsin Agric. Exp. Sta. Res. Report 41. 1969.

Site location details

ANDR: Andros, Bahamas (24°53'54.3" N, 77°54'25.2" W)

BERM: Bailey's Bay, Bermuda (32°15'49.9" N, 64°49'50.5" W)

BOCA: Bocas Del Toro, Panama (9°21'05.8" N, 82°15'27.8" W)

BONA: Lac Bay, Bonaire (12°06'44.3" N, 68°13'42.0" W)

CARR: Carrie Bow Cay, Belize (16°49'24.8" N, 88°06'16.2" W)

CAYM: Little Cayman, Cayman Islands (19°40'14.6" N, 80°03'21.3" W)

CORP: Corpus Christi, TX (27°55'47.9" N, 97°02'05.2" W)

CRYS: Crystal River, FL (28°42'50.4" N, 82°49'08.4" W)

ELEU: Eleuthera, Bahamas (25°27'53.5" N, 76°37'35.8" W)

GALV: Galveston, TX (29°02'41.8" N, 95°10'15.7" W)

JOES: St. Joe's Bay, FL (29°42'05.0" N, 85°19'34.6" W)

NAPL: Naples, FL (26°29'48.6" N, 82°09'40.0" W)

PUER: Puerto Morelos, Mexico (20°52'04.5" N, 86°51'35.4" W)

BCO-DMO Processing

- removed units from column headers
- converted latitude and longitude columns from degrees, minutes, seconds to decimal degrees
- latitude and longitude values rounded to 6 degrees of precision
- spaces removed from column names and replaced with underscores ("_")
- special characters removed from column names
- split the season field ("Spring 2019") into two separate fields for season ("Spring") and year ("2019")

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1737247

Deployments

None available yet

Instruments

Drying Oven

Supplied Name: Oven

Supplied Description:

For initial processing, belowground biomass was dried in a 60 C oven.

Instrument Type

Generic Name: Drying Oven

Generic Description:

a heated chamber for drying

Ion Chromatograph

Supplied Name: Phenomenex Luna NH2 HPLC Column

Supplied Description:

Determination of soluble carbohydrates was completed with a Phenomenex Luna NH2 (250 mm x 4.6 mm) HPLC.

Instrument Type

Generic Name: Ion Chromatograph

Generic Description:

Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
site	Site name / location; ANDR = Andros, Bahamas, BERM = Bailey's Bay, Bermuda, BOCA = Bocas Del Toro, Panama, BONA = Lac Bay, Bonaire, CARR = Carrie Bow Cay, Belize, CAYM = Little Cayman, Cayman Islands, CORP = Corpus Christi, Texas, CRYS = Crystal River, FL, ELEU = Eleuthera, Bahamas, GALV = Galveston, TX, JOES = St. Joe's Bay, FL, NAPL = Naples, FL, PUER = Puerto Morelos, Mexico	unitless	site
latitude	Site latitude in decimal degrees; a positive value indicates a Northern coordinate	decimal degrees	lat
longitude	Site longitude in decimal degrees; a negative value indicates a Western coordinate	decimal degrees	lon
season	Season sample collection took place	unitless	season
year	Year sample collection took place	unitless	year
plot	Plot ID number	unitless	Site_ID
clipping	Level of seagrass plot canopy clipping; no = no clipping, partial = half canopy removed, full = full canopy removed	unitless	treatment
nutrients	Level of nutrients; ambient=no added nutrients, enriched=added nutrients	unitless	treatment
cage	Level of cage; no=no cage, partial=partial 4-sided cage,full=full cage	unitless	treatment
glucose_percentage	Soluble carbohydrates (glucose) expressed as a percentage of belowground dry mass (%)	unitless	carbohydrates
sucrose_percentage	Soluble carbohydrates (sucrose) expressed as a percentage of belowground dry mass (%)	unitless	carbohydrates
fructose_percentage	Soluble carbohydrates (fructose) expressed as a percentage of belowground dry mass (%)	unitless	carbohydrates
total_nonstructural_carbohydrates_percentage	Non-structural carbohydrates percentage total (%)	unitless	carbohydrates

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Dataset: TEN Seagrass Soluble Carbohydrates