Dataset: Effect of phenotypic variation on dogwhelk morphology during eco-evolutionary field experiment

Name: Effect of phenotypic variation on dogwhelk morphology during an eco-evolutionary field experiment
Published: 2024-01-26
Keywords: Eco-evolutionary Dynamics, Nucella canaliculata, morphology, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.918546.1

Spatial Extent: N:38.323515 E:-123.078133 S:38.323515 W:-123.078133

Bodega Marine Reserve, California

Temporal Extent: 2021-07-20 - 2022-08-29

Project:

Coastal mosaics of local adaptation and the eco-evolutionary dynamics of a marine predator-prey interaction (Coastal Adaptation)

Principal Investigator:

Eric Sanford (University of California-Davis, UC Davis-BML)

Student:

Emily K. Longman (University of California-Davis, UC Davis-BML)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-01-26

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Effect of phenotypic variation on dogwhelk morphology during an eco-evolutionary field experiment

Abstract:

Although there is a growing body of work indicating that ecological and evolutionary processes can have reciprocal feedbacks on one another, few studies have tested these feedbacks in natural field settings at the community level. We tested the ecological consequences of selection on intra-population variation in dogwhelk drilling. We reared juvenile dogwhelks (Nucella canaliculata) on four early-life diet treatments (thin-shelled Mytilus trossulus, two treatments of M. californianus from two populations known to differ in adult shell thickness, and acorn barnacles) and then outplanted the dogwhelks to field cages to quantify the community effects of variation in drilling phenotype on mussel bed succession over a year. Changes in Nucella canaliculata morphology (change in length) were determined over the course of the experiment.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-01-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.918546.1	2024-01-26

Methods & Sampling

Dogwhelks (Nucella canaliculata) were reared in the lab from hatching on four early-life diet treatments (thin-shelled Mytilus trossulus, two treatments of M. californianus from two populations known to differ in adult shell thickness, and acorn barnacles). Adult dogwhelks were outplanted to field cages in July of 2021. The tidal height of each cage was measured relative to Mean Lower Low Water (MLLW) using a rotary laser level (DeWalt DW071). Five dogwhelks from the same family (i.e., dogwhelks from the same egg capsule cluster) and early-life diet treatment were placed in each of the cages. Prior to the outplant, the length of dogwhelks was measured with digital calipers, the sex was determined with visual inspection, and the snails were individually marked with small tags (Floy Tags). Cages were checked every 8 weeks for the next year. If a dogwhelk was dead, it was replaced with another dogwhelk from the same family by diet treatment. At the end of the experiment, the length of the snails was remeasured.

BCO-DMO Processing

- Imported original file "Effect of phenotypic variation on dogwhelk morphology during eco-evolutionary field experiment.xlsx" into the BCO-DMO system.
- Flagged "NA" as a missing data value (missing data are empty/blank in the final CSV file).
- Added columns for site Latitude and Longitude.
- Renamed fields/columns to comply with BCO-DMO naming conventions.
- Rounded the "Plot_Tidal_Height" to 5 decimal places.
- Saved the final file as "918546_v1_dogwhelk_morphology.csv".

Data Files

File
918546_v1_dogwhelk_morphology.csv (Comma Separated Values (.csv), 32.68 KB) MD5:0c173ce1d31dc4ff3c6f0f5b25ced02f Primary data file for dataset ID 918546, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1851462

Deployments

None available yet

Instruments

labeling tag

Supplied Name: Floy Tags

Supplied Description:

Floy Tags, Seattle, Washington, USA: Small individual numbered tags were affixed to each dogwhelk

Instrument Type

Generic Name: labeling tag

Generic Description:

Passive devices attached to captured organisms to specifically identify them when recaptured after release.

Levels and staffs

Supplied Name: Rotary laser level

Supplied Description:

DeWalt DW071: Rotary laser level used to measure plot tidal heights relative to Mean Lower Low Water (MLLW)

Instrument Type

Generic Name: Levels and staffs

Generic Description:

Optical instruments and graduated poles used in surveying to determine the elevation of a location relative to a datum level.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Block	Block number. Plots were in a block design such that 5 plots were near each other. Each block contained snails from the same family or egg capsule cluster, with each cage randomly assigned to contain snails from one of the four early-life diet treatments, plus a reference cage with no dogwhelks. Blocks were numbered sequentially.	unitless	sample
Plot_Number	Unique identifier for each plot.	unitless	sample
Plot_Tidal_Height	Plot tidal height relative to Mean Lower Low Water (MLLW).	meters (m)	tide
Family	Families (dogwhelks from the same egg capsules cluster were considered to be full or half siblings and classified as a family) were given a unique identifier.	unitless	sample
Site_Latitude	Latitude of the Bodega Marine Reserve field site.	decimal degrees	lat
Site_Longitude	Longitude of the Bodega Marine Reserve field site (negative values = West).	decimal degrees	lon
Treatment	Nucella canaliculata early-life diet treatment. Four treatments were used in this study: (1) a control diet of thin-shelled M. trossulus, (2) M. californianus from Soberanes Point, (3) M. californianus from Bodega Marine Reserve, and (4) acorn barnacles (Chthamalus dalli).	unitless	treatment
Family_x_Treatment	Each family by early-life diet treatment was given a unique identifier.	unitless	sample
Snail_ID	Each snail was given a unique identifier that represented the family by diet treatment.	unitless	sample
Snail_Tag	Small tags were affixed to the dogwhelks for easy identification in the field.	unitless	sample
Sex	Dogwhelk sex: male (M) or female (F).	unitless	no_bcodmo_term
Length_initial	Initial dogwhelk length prior to snail outplant.	millimeters (mm)	length
Length_final	Final dogwhelk length at the end of the year-long outplant.	millimeters (mm)	length
Length_change	Change in dogwhelk length over the course of the year-long field outplant experiment.	millimeters (mm)	length
Dead_vs_Replaced_vs_Never_found	D, NF, or R. Cages were checked every 8 weeks during each check as many dogwhelks as possible were located. Dead dogwhelks (D) were recorded and subsequently replaced (R) with another snail from the same family by diet treatment. At the end of the experiment, it was also noted if a snail was never found (NF).	unitless	sample_descrip
Date_dead_or_replaced	Date on which a dead dogwhelk was found or the date that a replacement dogwhelk was placed in the experimental cages.	unitless	sample_descrip