Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • BCO-DMO Processing Description
• Data Files
• Related Publications
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Carbon isotopes and concentrations of phytol:
Size-fractionated particle samples were collected using McLane WTS-LV in-situ pumps using four 142-millimeter (mm) diameter filter tiers. Size fractions reported here are as follows: 1.2-6 micrometers (μm) size fraction collected on two pre-combusted, stacked 1.2 μm glass fiber filters and 0.3-1.2 μm size fraction collected on two pre-combusted, stacked 0.3 μm glass fiber filters. Samples were stored at -80 degrees Celsius until processing. Chlorophyll was extracted from frozen or freeze-dried filter splits as part of a total lipid extraction using a mixture of chilled methanol, dichloromethane, and milliQ water (Bligh & Dyer, 1959; Sturt et al., 2004) using freeze/thaw, sonication, and vortexing/shaking to enhance extraction efficiency. Filter material was removed, and the total lipid extract (TLE) was further purified via liquid-liquid extraction against salt water and dried under N2. Lipid classes from each TLE were separated on silica gel mini columns (Bastow et al., 2007); reported here is the sum of the concentrations of phytol from chlorophyll in these fractions and the weighted average d13C value. Lipid classes were aliquoted by volume and saponified to cleave the phytol side chain from intact chlorophyll. Neutral lipids were obtained via liquid-liquid extraction from the basic mixtures and concentrated using a Turbovap evaporator. Quantitative aliquots were derivatized to trimethylsilyl (TMS) ethers and analyzed via gas chromatography mass spectrometry with a TG-5MS column. Samples containing sufficient phytol were analyzed for stable carbon isotope composition via gas chromatography-isotope ratio mass spectrometry equipped with a TG-5MS column. Phytol standards of a known δ13C value were derivatized alongside samples to account for isotope fractionation during the derivatization reaction as well as the δ13C value of added derivative carbon.

Carbon isotopes and bulk particulate organic carbon (POC, d13C POC):
Size-fractionated particle samples were collected using McLane WTS-LV in-situ pumps using four 142 mm diameter filter tiers. Size fractions reported here are as follows: 1.2-6 μm size fraction collected on two pre-combusted, stacked 1.2 μm glass fiber filters and 0.3-1.2 μm size fraction collected on two pre-combusted, stacked 0.3 μm glass fiber filters. Samples were stored at -80 degrees Celsius until processing. Filter splits were then freeze-dried, and carbonates were removed via acidification. Bulk POC concentration and isotope composition were measured using a Thermo Flash elemental analyzer coupled to a Conflo IV and MAT 253 Plus isotope ratio mass spectrometer (EA-IRMS, Thermo Scientific). These data are not blank corrected, but blanks were measured and are negligible relative to measured POC and d13C values.

Amino Acid analysis (d13C THAA):
Size-fractionated particle samples were collected using McLane WTS-LV in-situ pumps using four 142 mm diameter filter tiers. Size fractions reported here are as follows: 1.2-6 μm size fraction collected on two pre-combusted, stacked 1.2 μm glass fiber filters and 0.3-1.2 μm size fraction collected on two pre-combusted, stacked 0.3 μm glass fiber filters. Samples were stored at -80 degrees Celsius until processing. Quantitative splits were freeze‑dried, hydrolyzed, purified, derivatized, and analyzed for nitrogen and carbon isotope composition of individual amino acids. δ13C values of total hydrolysable amino acids (δ13CTHAA) were calculated as in McCarthy et al. (2013): δ13CTHAA = Σ (δ13CAA * mol%AA) where δ13CAA and mol%AA are the δ13C value and the molar percentage contribution of each individual amino acid, respectively. The standard deviation for each sample was calculated as the square root of the weighted average of variances of each individual amino acid.

Trophic Position (TP) from d15N-AA:
Size-fractionated particle samples were collected using McLane WTS-LV in-situ pumps using four 142 mm diameter filter tiers. Size fractions reported here are as follows: 1.2-6 μm size fraction collected on two pre-combusted, stacked 1.2 μm glass fiber filters and 0.3-1.2 μm size fraction collected on two pre-combusted, stacked 0.3 μm glass fiber filters. Samples were stored at -80 degrees Celsius until processing. Quantitative splits were freeze‑dried, hydrolyzed, purified, derivatized, and analyzed for nitrogen and carbon isotope composition of individual amino acids. POM trophic position was calculated from measured δ15N values of glutamic acid+glutamine (Glx) and phenylalanine (Phe) as in Chikaraishi et al. (2009): TP = (δ15NGlx - δ15NPhe - 3.4)/7.6 + 1 . TP propagated uncertainty was calculated as in Jarman et al. (2017).

- Imported original file "BIOSSCOPE_in-situ pump_chem data.xlsx" into the BCO-DMO system.
- Flagged "NaN" and "n/a" as missing data identifiers (missing data are empty/blank in the final CSV).
- Renamed fields (parameters) to comply with BCO-DMO naming conventions.
- Converted dates to YYYY-MM-DD format.
- Added columns for Latitude and Longitude.
- Saved the final file as "920443_v1_biosscope_in_situ_pump_chemical_data.csv".

The aim of BIOS-SCOPE is to expand knowledge about the BATS ecosystem and achieve a better understanding of ocean food web sources, sinks and transformations of DOM. Advances in knowledge and technology now poise us to investigate the specific mechanisms of DOM incorporation, oxidation and transformation by zooplankton and the distinct microbial plankton communities that have been discovered at BATS.

The overarching goal of the BIOS-SCOPE is to form and foster collaborations of cross disciplinary science that utilize a broad suite of genomic, chemical, ecological, and biogeochemical approaches to evaluate microbial process, structure and function on various scales. These scales will range from organism-compound and organism-organism interactions to large biogeochemical patterns on the ecosystem scale. For this purpose we have assembled a cross-disciplinary team including microbial oceanographers (Carlson and Giovannoni), a chemical oceanographer (Kujawinski), biological oceanographer / zooplankton ecologists (Maas and Blanco-Bercial) and microbial bioinformatician (Temperton) with the expertise and technical acuity that are needed to study complex interactions between food web processes, microbes and DOM quantity and quality in the oligotrophic ocean. This scientific team has a vision of harnessing this potential to produce new discoveries that provide a mechanistic understanding of the carbon cycle and explain the many emergent phenomenon that have yet to be understood.

For additional details:

• BIOS-SCOPE Narrative: 
  https://datadocs.bco-dmo.org/docs/302/BIOSSCOPE/data_docs/BIOS-SCOPE_Narrative_FINAL.pdf
• Physical Framework:
  https://datadocs.bco-dmo.org/docs/302/BIOSSCOPE/data_docs/Physical_Framework.pdf

BIOSSCOPE I: November 1st, 2015 through October 31st, 2020
Current: November 1st, 2020 to October 31st, 2025