Dataset: Survivorship measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA)

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.924088.1Version 1 (2024-04-01)Dataset Type:Other Field ResultsDataset Type:experimental

Principal Investigator: Hans G. Dam (University of Connecticut)

Co-Principal Investigator: Hannes Baumann (University of Connecticut)

Co-Principal Investigator: Michael Finiguerra (University of Connecticut)

Co-Principal Investigator: Melissa Pespeni (University of Vermont)

Scientist: Reid Brennan (University of Vermont)

Student: James deMayo (University of Connecticut)

Student: Gihong Park (University of Connecticut)

Technician: Lydia Norton (University of Connecticut)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Response of marine copepods to warming temperature and ocean acidification (Copepod Response to Warming Temp and OA)


Abstract

These data include survivorship measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every second generation between F0 and F4 for all treatments and F11 for AM and OWA. Data were collected as the proportion of surviving individuals on any day (x) relative to the starting numb...

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Three hundred copepods were collected in April of 2018 from eastern Long Island Sound, Connecticut, USA (41.3°N, 72.0°W) and raised for one year (~12 generations) (14 degrees Celsius (°C), 400 microatmospheres (μatm) CO2, 30 ‰ salinity, 12:12 hours light:dark) as stock cultures to limit maternal effects (Falconer, 1989, Introduction to Quantitative Genetics). Three resulting stock cultures with >2,000 individuals each were combined and then split evenly into three groups for each of the four treatments. Groups were acclimatized within a generation to temperature (15°C or 13°C, 1°C per day) and pCO2 (1000 μatm, 100 μatm per day, OA treatments only). Groups seeded the F0 individuals for 7-10 days yielding ~15,000 eggs per treatment. Resulting F0 eggs and nauplii were combined for each treatment, redistributed among three replicate cultures, and returned to their respective experimental conditions. The experimental environmental conditions were: 1) Ambient control (AM): 13°C, 400 µatm CO2, pH = 8.2; 2) Ocean Acidification (OA): 13°C, 1000 µatm CO2, pH = 7.85; 3) Ocean Warming (OW): 15°C, 400 μatm CO2, pH = 8.2; 4) Combined warming and acidification (OWA): 15°C, 1000 μatm CO2, pH = 7.85. Copepods were fed equal proportions of the live phytoplankters Tetraselmis sp., Rhodomonas sp., and Thalassiosira weissflogii every 48-72 hours to achieve food-replete conditions (≥600 micrograms (μg) Carbon per liter (L)) (Feinberg and Dam, 1998. Marine Ecology Progress Series), deliberately raised under ambient conditions to avoid confounding effects of possible food quality changes.

Survival was measured from nauplius 1 (N1) to copepodid 6 (C6; adult). For a given generation, all adults from the previous generation were removed from the culture and allowed to lay eggs in food-replete media for 48 hours. Resulting nauplii were chosen for tracking survival. Unhatched eggs and any nauplii not chosen for survival analysis were returned to their respective cultures for continued population maintenance. To measure survival, three 250 milliliter (mL) beakers for each replicate culture were supplied with 25 randomly chosen N1 nauplii each and housed in the plexiglass enclosure (n= 9 per treatment). Copepods were checked every 48-72 hours. The number of dead, live, and missing copepods was recorded for each beaker along with developmental stage (nauplius, copepodite, adult female, or adult male). Nauplii were grown with media at levels of 250 μg C L-1 for the first four days to prevent overgrowth of phytoplankton and allow for adequate nauplii grazing. Then, copepods were grown with food-replete media. For food limitation experiments in F11, the three beakers for each replicate culture were split evenly between the three food concentrations. Food media was replaced on monitoring days. Average survival probabilities were calculated for each replicate culture at each generation as the proportion of surviving individuals on monitoring days.


Related Datasets

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Dataset: Ocean warming & acidification experiment: Acartia hudsonica body size
Relationship Description: These datasets result from the same set of experiments.
Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Body size measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-03 doi:10.26008/1912/bco-dmo.924236.1
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Dataset: Ocean warming & acidification experiment: Acartia hudsonica development time
Relationship Description: These datasets result from the same set of experiments.
Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Development (i.e. maturation) time measurements for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-03 doi:10.26008/1912/bco-dmo.924206.1
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Dataset: Ocean warming & acidification experiment: Acartia hudsonica egg production and hatching success
Relationship Description: These datasets result from the same set of experiments.
Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Egg production rate (EPR) and egg hatching success (HS) data for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-02 doi:10.26008/1912/bco-dmo.924126.1
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Dataset: Ocean warming & acidification experiment: Acartia hudsonica population fitness
Relationship Description: These datasets result from the same set of experiments.
Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Population fitness measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-29 doi:10.26008/1912/bco-dmo.923960.1
IsRelatedTo

Dataset: Ocean warming & acidification experiment: Acartia tonsa population fitness
Relationship Description: These datasets result from the same set of experiments.
Dam, H. G., Baumann, H., Finiguerra, M., Pespeni, M., Brennan, R. (2024) Population fitness measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-03-28 doi:10.26008/1912/bco-dmo.923908.1

Related Publications

Results

deMayo, J. A., Brennan, R. S., Pespeni, M. H., Finiguerra, M., Norton, L., Park, G., Baumann, H., & Dam, H. G. (2023). Simultaneous warming and acidification limit population fitness and reveal phenotype costs for a marine copepod. Proceedings of the Royal Society B: Biological Sciences, 290(2006). https://doi.org/10.1098/rspb.2023.1033
Software

R Core Team (2020). R: A language and environment for statistical computing. R v4.0.2. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/