Dataset: Shoot measurements (sheath length and width) for the eelgrass (Zostera marina) shoots sampled for whole genome sequencing collected from Bodega and Tomales Bay, CA, USA from July to September 2019

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.924808.1Version 1 (2024-04-10)Dataset Type:Other Field Results

Principal Investigator: John J. Stachowicz (University of California-Davis)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Using genomics to link traits to ecosystem function in the eelgrass Zostera marina (ZosteraEcoGenomics)


Abstract

This dataset provides shoot measurements for the shoots sampled for whole genome sequencing for Tomales Bay and Bodega Harbor (see related datasets for access to sequence data). Sheath width is measured on the terminal shoot using calipers at the abscission point (point where leaves emerge from the sheath). Sheath length is measured using a ruler to the nearest mm from the base to the abscission point. These data were used to record any differences in plant phenotype among the populations sample...

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We collected 2 to 3 shoots attached by a rhizome from fifteen putative genets (separated by approximately 5 to 10 meters) at 14 sites across Tomales Bay and Bodega Harbor from a height below 0.0 mean lower low water (MLLW) (i.e., not sampling the uppermost or lowermost vertical distribution of Zostera marina). For two of the sites sampled in Bodega Harbor (Mason's Marina and Westside Park), we also collected a deeper set of specimens (at least -0.6 meters below MLLW) to test for differences between shallower versus deeper plants. We transported plants back to the University of California, Davis in a cooler with ice packs, and stored them for no more than 1 day in a recirculating seawater table before measuring the terminal shoot sheath width using calipers and sheath length using a ruler. The sheath width was measured at the abscission point and the length from the abscission point to the base of the shoot. Once measurements were made, we dissected out the tissue from within the leaf sheath and flash-froze them in liquid nitrogen and stored at -80 degrees Celsius (°C).


Related Datasets

IsRelatedTo

Dataset: Whole Genome Sequencing of Eelgrass Bodega and Tomales Bay
Relationship Description: These datasets were collected concurrently as part of a study of parallel genomic adaptation of Zostera marina in northern California estuaries published in Scheibelhut, et al. (2023).
Stachowicz, J. J. (2024) Sample collection information and sequence accessions at the National Center for Biotechnology Information (NCBI) for whole genome sequencing of eelgrass (Zostera marina) collected at Bodega and Tomales Bay, CA, USA from July to September 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-10 doi:10.26008/1912/bco-dmo.924786.1
IsRelatedTo

Dataset: Temperature Data From Tomales Bay and Bodega Harbor
Relationship Description: These datasets were collected concurrently as part of a study of parallel genomic adaptation of Zostera marina in northern California estuaries published in Scheibelhut, et al. (2023).
Stachowicz, J. J. (2024) Temperature data recorded using HOBO Pendant MX2201 loggers deployed at 14 sites in Tomales Bay and Bodega Harbor during August 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-09 doi:10.26008/1912/bco-dmo.924671.1

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