Dataset: STING I and STING II extracted chlorophyll a and pheophytin

Name: Chlorphyll a and pheophytin from two cruises performed as part of the STING project from R/V Atlantic Explorer AE2305 and R/V Endeavor EN704 in the Gulf of Mexico near Florida from February to July 2023
Published: 2024-05-28
Keywords: extracted chlorophyll, extracted pheophytin, West Florida shelf, oceans

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Cite This Dataset

Spatial Extent: N:28.498467 E:-82.515283 S:26.13205 W:-86.566932

West Florida Shelf centered around Tampa Bay (~27 N) out to just past the edge of the Florida escarpment (~ -86 E; 3000 m)

Temporal Extent: 2023-02-20 - 2023-07-12

Project:

Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes (Gulf of Mexico DON and Fe)

Principal Investigator:

Phoebe Dreux Chappell (University of South Florida, USF)

Student:

Kristina Confesor (University of South Florida, USF)

BCO-DMO Data Manager:

Lynne M. Merchant (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-05-28

Restricted:

Validated:

Current State:

Preliminary and in progress

Chlorphyll a and pheophytin from two cruises performed as part of the STING project from R/V Atlantic Explorer AE2305 and R/V Endeavor EN704 in the Gulf of Mexico near Florida from February to July 2023

Abstract:

These data include the average (n=3 replicates per measurement) measured chlorphyll a and pheophytin from two cruises performed as part of the STING project. STING I (AE2305) was aboard the R/V Atlantic Explorer from 2023-02-20 to 2023-03-06. STING II (EN704) was aboard the R/V Endeavor from 2023-07-01 to 2023-07-12. 300 to 4300 ml was filtered onto GF/F filters (nominal pore size 0.7 um) and kept frozen (at -20 degrees C) in the dark until analysis. Filters were extracted overnight in 90% acetone before analysis on a Trilogy Fluorometer with the chl a extracted acidification module calibrated using Turner Chl a standards. Following the initial fluorescence reading, 20 ul of 10% HCl was added and another fluorescence reading was recorded.

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Methods & Sampling

Whole water was collected using three methods: tow-fish, surface pump deployed on a metal-free line, or ctd rosette. Whole water (300 - 4300 ml) was filtered onto 25 mm GF/F filters (nominal pore size 0.7 um) using a GAST vacuum pump and frozen at -20 degrees C until analysis. Filters were extracted overnight (16-18 hrs) in 90% acetone with a 10% HCl acidificaiton step following the extraction protocol of Strickland and Parsons 1972 (Strickland, J. D. H., and T. R. Parsons. 1972. A practical handbook of seawater analysis. Fisheries Research Board of Canada.) and analyzed on a fluorometer.

The surface pump on STING I was a Wilden Pro-Flo Series P100 PTPE pump. The surface pump on STING II and for all tow-fish samples was an Almatec E15TTT pump. All pump samples were pumped through Teflon tubing. The CTD samples were collected via 10 L Niskin bottles. The fluorometer was a Turner Designs Trilogy Fluorometer with the chl a acidification method module. Calibration was done using chl a standards from Turner.

Data Processing Description

Duplicates from each sample were averaged together.

BCO-DMO Processing

1. Loaded submitted file STING_Chl_ToSubmit.csv into BCO-DMO data processing system laminar.
2. Converted date from the format %d-%b-%Y to an ISO date format of %Y-%m-%d
3. Renamed parameters with units in their name to remove the units from the text. For example, renamed 'Pheophytin_ugperL' to 'Pheophytin'
4. Reordered parameter columns so that location and date metadata comes before data results.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-2341997

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AE2305	STING	2023-02-18	R/V Atlantic Explorer	Angela N. Knapp (Chief Scientist)	data info
EN704		2023-07-01	R/V Endeavor	Angela N. Knapp (Chief Scientist)	data info

Instruments

Niskin bottle

Supplied Name: Niskin bottles

Supplied Description:

10 L Niskin bottles

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Pump

Supplied Name: surface pump

Supplied Description:

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Turner Designs Trilogy fluorometer

Supplied Name: Turner Designs Trilogy Fluorometer

Supplied Description:

Turner Designs Trilogy Fluorometer with the chl a acidification method module. Calibration was done using chl a standards from Turner.

Instrument Type

Generic Name: Turner Designs Trilogy fluorometer

Acronym: Turner Trilogy

Generic Description:

The Trilogy Laboratory Fluorometer is a compact laboratory instrument for making fluorescence, absorbance, and turbidity measurements using the appropriate snap-in application module. Fluorescence modules are available for discrete sample measurements of various fluorescent materials including chlorophyll (in vivo and extracted), rhodamine, fluorescein, cyanobacteria pigments, ammonium, CDOM, optical brighteners, and other fluorescent compounds.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	which cruise sample was collected: STINGI or STINGII	unitless	cruise_id
Station	Station identifier	unitless	sta_id
Lat	Sampling location latitude, south is negative	decimal degrees	lat
Lon	Sampling location longitude, west is negative	decimal degrees	lon
Depth	Depth of sample collection	meters (m)	depth
Date	Date of sample collection Format: %Y-%m-%d	unitless	date
STING_ID	Project specific identification number, which is unique to each sample collection	unitless	sample
Collection	Method of collection of water sample. fish = towfish, koubapump = stationary teflon pump, tmctd = trace metal ctd rosette, shipctd = conventional ctd rosette	unitless	sampling_method
Type	Type of sample collection: surface, chl max = chlorophyll maximum, profile = other depth	unitless	sample_type
Chla	Chlorophyll a concentration	micrograms/liter (ug/l)	chl_a
Pheophytin	Pheophytin concentration	micrograms/liter (ug/l)	phaeo

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Dataset: STING I and STING II extracted chlorophyll a and pheophytin