Synechococcus Growth – Axenic Synechococcus WH8102 (open ocean strain) and WH5701 (coastal strain) were obtained from the National Center for Marine Algae and Microbiota (NCMA, Bigelow Laboratories, East Boothbay, Maine). Both strains were grown in batch culture using SN media (Waterbury et al. 1986) made with aged, filtered (0.2 µm), and autoclaved (120°C, 30 minutes) seawater from station ALOHA (A Long-term Oligotrophic Habitat Assessment). All cultures were incubated at 25°C on a 12h:12h light cycle at 130 µmol m^-1 s^-1 in sterile culture flasks with a vent cap (0.22 µm hydrophobic membrane).

Growth on DOP Substrates – The capacity of Synechococcus WH8102 and WH5701 to grow on DOP as a sole phosphorus source was tested in -Pi SN media amended with a single DOP substrate (45 µmol L-1 P, final concentration, Waterbury et al. (1986)). Representative DOP compounds included the P-monoesters glucose-6-phosphate (Glc-6-P) and adenosine 5’-monophosphate (AMP); the P-diester Bis(4-methylumbelliferyl) phosphate (BisMUF-P); the short and long chain polyphosphates: 3-polyphosphate (3-PolyP) and 45-polyphosphate (45-PolyP); the P-monoester and P-anhydride containing adenosine 5’-triphosphate (ATP); and the phosphonates: 4-nitrophenyl phenylphosphonate (4-NpPn), 2-aminomethylphosphonic acid (2-AEPn), methylphosphonic acid (MPn), and ethylphosphonic acid (EPn). Two separate experiments were carried out in triplicate to test growth on 1) P-monoester and PolyP substrates and 2) a P-diester and phosphonates. Two control treatments (culture grown in +Pi and -Pi media) were carried out in triplicate for each experiment. IVF was measured daily in each treatment over ~20 days, and cell axenicity was tested every ~5 days.

Organism identifiers (Life Science Identifier, LSID):
Synechococcus, urn:lsid:marinespecies.org:taxname:160572

929212_v1_syn-growth-dop-ivf.csv