Dataset: Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC)
Data Citation:
Breitbart, M., Furman, B., Rosario, K., Kernbach, M., Lim, S. J. (2024) Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb 2022 to Oct 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-07-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.931469.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.26008/1912/bco-dmo.931469.1
Spatial Extent: N:29.99911 E:-81.799295 S:24.560981 W:-84.35056
Western Florida, USA
Temporal Extent: 2022-02-04 - 2023-10-03
Project:
Principal Investigator:
Mya Breitbart (Florida Fish and Wildlife Commission, FWC)
Co-Principal Investigator:
Bradley Furman (Florida Fish and Wildlife Commission, FWC)
Karyna Rosario (University of South Florida, USF)
Scientist:
Meredith Kernbach (University of South Florida, USF)
Shen Jean Lim (University of South Florida, USF)
Technician:
Anthony J Gross (University of South Florida, USF)
Data Manager:
Dana Stuart Gerlach (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Audrey Mickle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2024-07-02
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb 2022 to Oct 2023
Abstract:
This dataset includes Sanger sequence references for 40 Thalassia testudinum samples collected during systematic seagrass surveys from multiple Florida sites, including Terra Ceia Aquatic Preserve, Tampa Bay seagrass sites S1T5 and S3T8 (Lassing Park), Panacea located in the Florida Panhandle, and Florida Keys sites including Bush Key, Garden Key, Marquesas Key, and Key West.
We investigated potexvirus distribution in seagrasses using a degenerate reverse transcription polymerase chain reaction (RT-PCR) assay originally designed to capture potexvirus diversity in terrestrial plants. The assay, which implements Potex-5 and Potex-2RC primers, successfully amplified a 584 nt RNA-dependent RNA polymerase (RdRp) fragment from TVX-infected seagrasses. Following validation, we screened 74 opportunistically collected, apparently healthy seagrass samples for potexviruses using this RT-PCR assay.
Potexvirus PCR products were successfully generated from Thalassia testudinum samples. Sequences from these products are deposited in NCBI GenBank under the accession numbers OR827692-OR827705, OR854648, OR863396, OR879052-OR879056, and PP430548-PP430571.