Dataset: Virus production at a hydrothermal vent site
View Data: Data not available yet
Data Citation:
Tinta, T., Krause, N., Bright, M., Winter, C. (2024) Virus and prokaryote abundances from experiments conducted with samples collected at a hydrothermal vent site by ROV SuBastian during R/V Falkor (too) expedition FKt230627 along the East Pacific Rise in July of 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-08-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/936224 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
Spatial Extent: N:9.840335 E:-104.291555 S:9.839953 W:-104.291922
East Pacific Rise (Hydrothermal vent site Tica, ~2515m depth)
Temporal Extent: 2023-07-06 - 2023-07-23
Project:
The Underworld of Hydrothermal Vents
(Vent Underworld)
Principal Investigator:
Christian Winter (University of Vienna)
Co-Principal Investigator:
Monika Bright (University of Vienna)
Scientist:
Tinkara Tinta (National Institute of Biology, Slovenia)
Student:
Nicole Krause (University of Vienna)
BCO-DMO Data Manager:
Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2024-08-23
Restricted:
No
Validated:
No
Current State:
Data not available
Virus and prokaryote abundances from experiments conducted with samples collected at a hydrothermal vent site by ROV SuBastian during R/V Falkor (too) expedition FKt230627 along the East Pacific Rise in July of 2023
Abstract:
We used the virus-dilution technique to quantify lytic virus production at nine sites across vent, sub-vent, and non-vent habitats along the East Pacific Rise during expedition FKt230627 aboard R/V Falkor(too). This technique leverages the density-dependent nature of viral infection, preventing new infections by dilution. Samples were collected via ROV SuBastian, sequentially filtered to remove larger particles, and concentrated using tangential-flow filtration. Duplicate incubations were set up with prokaryotic concentrate and virus-free water, pressurized to 250 bar, and incubated in the dark at in situ temperatures for 30 hours. Subsamples were taken every six hours for enumeration of prokaryotes and viruses via flow cytometry. Parallel experiments were conducted at surface pressure to assess the impact of pressure on virus production rates. The methodology ensures observed increases in viral abundance are due to pre-existing infections. The experiments were conducted aboard R/V Falkor(too) between 5-22 July 2023 by Tinkara Tinta and Nicole Krause. Prokaryotes and viruses were enumerated flow-cytometrically back in the lab by Christian Winter.