Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
  • BCO-DMO Processing Description
  • Problem Description
• Data Files
• Supplemental Files
• Related Publications
• Parameters
• Instruments
• Deployments
• Project Information
• Program Information
• Funding

The primary data file "936824_v1_bait_dissolved_fe_and_isotopes.csv" includes data from all four cruises. Data from each cruise is also provided separately in the attached Supplemental Files (csv format). The Supplemental Files include the unique GEOTRACES barcodes assigned to each parameter from each cruise. These barcodes are used by the GEOTRACES program for data tracking purposes.

Water-column samples for trace metal measurements were collected from the Bermuda Atlantic Time-Series (BATS) site (31°40'N, 64°10'W) and adjacent BATS spatial stations during cruises in March (spring), May (early summer), August (late summer) and November (fall) 2019 aboard R/V Atlantic Explorer and R/V Endeavor.

Seawater samples and hydrographic data were collected using a trace-metal clean conductivity-temperature-depth sensor (SBE 19 plus, SeaBird Electronics) mounted on a custom-built trace-metal clean carousel (SeaBird Electronics) fitted with custom-modified 5-liter (L) Teflon-lined external-closure Niskin-X samplers (General Oceanics) and deployed on an Amsteel non-metallic line. On the August cruise, we also collected near-surface samples (small boat) (~0.3 meters (m) depth) in a Niskin-X sampler deployed from an inflatable dinghy ~500 m upwind of the research vessel, to avoid contamination from the ship. After recovery, the seawater samples were filtered through pre-cleaned 0.2-micrometer (μm) pore AcroPak Supor filter capsules (Pall) using filtered nitrogen gas inside a shipboard clean laboratory (Sedwick et al., 2020; Tagliabue et al., 2023) into acid-cleaned 1L LDPE bottles.

Samples were acidified back on shore at the University of South Florida (USF) using 1.2 milliliter (mL) 10 M Teflon-distilled HCl per liter of sample and stored for at least 12 months before processing. Seawater samples were then processed for dissolved iron (Fe) isotope and concentration analysis at the University of South Florida following Sieber et al. (2019), modified from Conway et al. (2013). Briefly, an Fe double-spike was added prior to batch extraction using Nobias PA-1 chelating resin, followed by purification by anion-exchange chromatography using AG-MP1 resin. Concentration and isotope analyses were performed on a Thermo Neptune Plus MC-ICPMS in the Tampa Bay Plasma Facility at the University of South Florida using the double spike technique via a ~100 microliters per minute (uL min-1) PFA nebulizer and Apex Ω introduction introduction system, Pt Jet Sampler cone and an Al X skimmer cone.

Fe stable isotope ratios are expressed in delta notation (δ56Fe) relative to the IRMM-014 standard. A secondary Fe standard, NIST-3126, was analyzed over 44 sessions to provide an estimate of long-term instrumental precision. We obtain a value of +0.36 ± 0.05‰ (2SD, n = 524; runs = 37), in agreement with consensus values (Hunt et al. 2022; Conway et al., 2013). Therefore, we consider a 2SD uncertainty of 0.05‰ as an estimate of analytical precision, and have applied it to all samples, except for low concentration samples where the larger internal error is considered a more conservative estimate of uncertainty. Concentrations were calculated using the isotope dilution technique based on on-peak blank, interference and mass-bias corrected 57Fe/56Fe ratios measured simultaneously with isotope analysis. We express uncertainty (1SD) on Fe concentrations as 2%, based on Conway et al. (2013).

Data were processed in Microsoft Excel using an-in house double spike reduction scheme from Sieber et al. (2021), based on Siebert et al. (2001).

To create the combined data file:
- Imported 4 original files into the BCO-DMO system: "AE1909_dataTemplate.xlsx", "AE1921_dataTemplate.xlsx", "AE1930_dataTemplate.xlsx", and "EN631_dataTemplate.xlsx".
- Renamed fields to comply with BCO-DMO naming conventions.
- Renamed "SD2_Fe_56_54_D_DELTA_BOTTLE" to "SD1_Fe_56_54_D_DELTA_BOTTLE" (AE1909 file).
- Concatenated data from separate cruises into a single data file; added column for Cruise_ID.
- Converted date column to YYYY-MM-DD format.
- Removed empty columns: Event_ID, Gear_ID, Start_Time_UTC, End_Date_UTC, End_Time_UTC, End_Latitude, End_Longitude, Rosette_Position.
- Saved the final file as "936824_v1_bait_dissolved_fe_and_isotopes.csv".

For the supplemental files:
- Imported 4 original files into the BCO-DMO system: "AE1909_dataTemplate.xlsx", "AE1921_dataTemplate.xlsx", "AE1930_dataTemplate.xlsx", and "EN631_dataTemplate.xlsx".
- Saved the files in CSV format.

NSF and NERC Award Abstract:
Iron is an essential nutrient for the growth of phytoplankton in the oceans. As such, iron plays key roles in regulating marine primary production and the cycling of carbon. It is thus important that models of ocean biology and chemistry consider iron, in order to explore past, present and future variations in marine productivity and the role of the ocean in the global carbon cycle. In this joint project involving researchers in the U.S. and the U.K., supported by both NSF and the Natural Environment Research Council (U.K.), field data from the Bermuda Atlantic Time-series Study (BATS) region will be combined with an established, state-of-the-art ocean biogeochemical model. By leveraging the known seasonal-scale physical, chemical and biological changes in the BATS region, the oceanographic context provided by the BATS core data, and an existing model of the regional physical circulation, the proposed study will yield process-related information that is of general applicability to the open ocean. In particular, the proposed research will focus on understanding the atmospheric input, biological uptake, regeneration and scavenging removal of dissolved iron in the oceanic water column, which have emerged as major uncertainties in the ocean iron cycle. The project will include significant educational and training contributions at the K-12, undergraduate, graduate and postdoctoral levels, as well as public outreach efforts that aim to explain the research and its importance.

The ability of ocean models to simulate iron remains crude, owing to an insufficient understanding of the mechanisms that drive variability in dissolved iron, particularly the involvement of iron-binding ligands, colloids and particles in the surface input, biological uptake, regeneration and scavenging of dissolved iron in the upper ocean. Basin-scale data produced by the GEOTRACES program provide an important resource for testing and improving models and, by extension, our mechanistic understanding of the ocean iron cycle. However such data provide only quasi-synoptic 'snapshots', which limits their utility in isolating and identifying the processes that control dissolved iron in the upper ocean. The proposed research aims to provide mechanistic insight into these governing processes by combining time-series data from the BATS region with numerical modeling experiments. Specifically, seasonally resolved data on the vertical (upper 2,000 meters) and lateral (tens of kilometers) distributions of particulate, dissolved, colloidal, soluble and ligand-bound iron species will be obtained from the chemical analysis of water column samples collected during five cruises, spanning a full annual cycle, shared with the monthly BATS program cruises. These data, along with ancillary data from the BATS program, will be used to test and inform numerical modeling experiments, and thus derive an improved understanding of the mechanisms that control the distribution and dynamics of dissolved iron in the oceanic water column.

This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

This is a project jointly funded by the National Science Foundation’s Directorate for Geosciences (NSF/GEO) and the National Environment Research Council (NERC) of the United Kingdom (UK). 

NSF Award Abstract:
Iron (Fe) is a crucial nutrient for microbial growth in the oceans, impacting the carbon cycle and the climate system, but Fe does not dissolve readily in seawater and so its availability limits phytoplankton growth over much of the surface oceans. One of the most significant ways by which Fe reaches the surface oceans is through deposition of wind-blown dust; however, for this Fe to be available for biological growth, it must dissolve and be kept in solution bound to organic molecules. Despite this known importance, the mechanisms of dust dissolution and the identity of the organic molecules keeping Fe in solution remain poorly understood. This study will focus on using laboratory and field experiments to better understand how natural organic molecules present in seawater enhance the release of Fe from Saharan desert dust, the organic molecules produced by microbes in response to dust, the isotopic fractionation of Fe associated with dust dissolution, and ultimately the role of ligand-mediation dissolution in determining the role of dust in the marine Fe cycle. For outreach activities, the proposers would tutor refugee high school students from Africa/Asia in math and science to help them get integrated into the U.S. school system and discuss science with the public by participation in the St. Petersburg Science Festival. Three graduate students and undergraduate interns would be supported and trained as part of this project.

Scientists from the University of South Florida and Oregon State University will collect natural North Atlantic dust at the Tudor Hill Tower on Bermuda over a yearly cycle and characterize the weekly bulk and water-soluble Fe isotopic and elemental composition of the dust. Appropriate subsamples of this dust, representing both the Saharan and non-Saharan (anthropogenically-influenced) seasons will then be dissolved in seawater, with dissolution mediated by ligands with a range of Fe binding stability constants (natural ligands as well as defined marine siderophores). Incubations of dust will also be carried out with natural biological communities collected from the Gulf of Mexico and at the Bermuda Atlantic Time Series site in the North Atlantic. The investigators will characterize the amount and isotopic signature of the Fe released in each experiment, the microbial community response to dust in the incubations (DNA, cell counting and identification), and the ligands present in the natural organic matter used for experiments and produced in the incubations. Comparison of the abiotic and biological experiments will allow investigation of the magnitude of the biological effects on dust dissolution. The experiments will allow the investigators to provide a mechanistic understanding of factors that affect dust dissolution rates, especially the role of different specific organic ligands in enhancing and stabilizing the release of iron in the surface ocean, and the net signature of iron released. From this, the investigators will provide new constraints for the marine iron isotope cycle in the ocean, specifically testing the hypothesis that the net release of Fe from dust is isotopically heavy due to complexation with organic ligands, but that the size of this fractionation depends on the binding strength and presence of different ligands. The outcomes of this process study will provide a framework for models and interpretation of large scale field studies such as GEOTRACES, as well as enhancing the research community's ability to interpret aerosol and oceanic iron isotope data.

This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.

GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;

* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and

* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.

GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.

Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.