Dataset: Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation in wild-caught fish in Long Island Sound in Sept of 2022 to Apr of 2023

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.938012.1Version 1 (2024-09-23)Dataset Type:Other Field Results

Principal Investigator: Hannes Baumann (University of Connecticut)

Student: Max D. Zavell (University of Connecticut)

Data Manager: Amber D. York (Woods Hole Oceanographic Institution)


Project: Collaborative research: The genomic underpinnings of local adaptation despite gene flow along a coastal environmental cline (GenomAdapt)


Abstract

This dataset contains adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation in wild-caught fish in Long Island Sound (September 2022 to April 2023). This dataset includes data from fish collected concurrently with those used in a related mesocosm experiment (run Oct 2022 to Apr 2023), with fish collected at the same reef (see 'Related Datasets' section). Study description: We experimentally examined overwintering potential of adult Black Sea Bass (Centropristi...

Show more

17 adult specimens were collected by boat off Stonington Borough, CT (41° 20’37.8” N 71° 54’51.4” W) between September 15th and October 10th, 2022 to act as a wild ‘pre-migratory’ baseline (‘fall’, n = 17) and an additional 21 individuals were collected from the same location on April 27th 2021 to act as a wild “post-migratory” baseline (‘spring’, n = 21). Upon collection, individuals were immediately euthanized with MS-222 and measured for length (TL; 33.7 ± 4.4 cm), body depth (BD; 8 ± 0.9 cm), and wet weight (wW; 532.6 ± 227 g). During dissections, the stomach was removed to calculate a stomachless whole weight to standardize for consumed prey items, and the liver and gonad was then removed, individually weighed (0.01 g) and frozen at -20°C for future lipid extractions. A subsample of dorsally located white muscle tissue was also removed and frozen.

For all adults, we calculated gonadosomatic (GSI; %) and hepatosomatic (HSI; %) indices using stomachless fish mass (wW – stomach mass), to standardize for stomach contents, as {e.g., 100 × [(gonad or liver mass, g) / (stomachless fish mass)].

We quantified gonad, liver, and white muscle, storage lipid, lean-mass, and ash weights of each surviving experimental individual and baseline specimen. Samples were frozen at -50°C for 1 week and remeasured for whole body dry weight (dWb, 0.001 g). Following published protocols (Schultz and Conover 1997, Guo et al. 2021, 2022, Zavell et al. 2023), dried specimens were loaded into preweighed Alundum medium-porosity extraction thimbles and transferred to a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for 3.5 h to extract all metabolically available lipids. Samples were then dried overnight (60°C) and re-measured, with the change in pre- and post-extraction weights (ΔdW), representing the storage-lipid fraction (dWLipid). Samples were then muffle furnaced for 4 h at 550°C and reweighed with ΔdW, representing the lean-mass fraction (dWLean) and the remaining mass represents the inorganic fraction (dWAsh). 

Organism Identifier (LSID, Life Sciences Identifier)
Centropristis striata, urn:lsid:marinespecies.org:taxname:159348


Related Datasets

IsRelatedTo

Dataset: Adult black sea bass winter survival and lipid dynamics: Laboratory-mesocosm experiment
Relationship Description: Data from the same study. Fish were collected from the same reef.
Baumann, H., Zavell, M. D. (2024) Adult Black Sea Bass (Centropristis striata) winter survival and lipid accumulation under varying diet and temperature conditions from a laboratory mesocosm experiment (Oct 2022 to Apr 2023) with individuals collected in Long Island Sound. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-09-23 doi:10.26008/1912/bco-dmo.938004.1

Related Publications

Results

Zavell, M.D., Mouland, E.P., Barnum, D.L., Matassa, C.M., Schultz, E.T., and Baumann, H. (Submitted). Can adult Black Sea Bass overwinter in Long Island Sound, USA? Submitted to Marine and Coastal Fisheries
Methods

Fulton, T. W. "The Rate of Growth of Fishes. 20th Annual Report of the Fishery Board of Scotland." (1902): 326-446.
Methods

Guo, L. W., Jordaan, A., Schultz, E. T., & McCormick, S. D. (2022). Identification of supraoptimal temperatures in juvenile blueback herring (Alosa aestivalis) using survival, growth rate and scaled energy reserves. Conservation Physiology, 10(1). https://doi.org/10.1093/conphys/coac022
Methods

Guo, L. W., McCormick, S. D., Schultz, E. T., & Jordaan, A. (2021). Direct and size‐mediated effects of temperature and ration‐dependent growth rates on energy reserves in juvenile anadromous alewives (Alosa pseudoharengus). Journal of Fish Biology, 99(4), 1236–1246. Portico. https://doi.org/10.1111/jfb.14824
Methods

Ricker, W. E. (1975). Computation and interpretation of biological statistics of fish populations. Fish. Res. Board Can. Bull., 191, 1-382.