| Contributors | Affiliation | Role |
|---|---|---|
| McKay, Robert Michael | University of Windsor | Principal Investigator |
| Bullerjahn, George S. | Bowling Green State University (BGSU) | Co-Principal Investigator |
| Hudson, Neve C | University of Windsor | Student |
| Anderson, James T. | United States Coast Guard (USCG) | Contact |
| Ballard, Zachary | United States Coast Guard (USCG) | Contact |
| Mickle, Audrey | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Lake Erie Center for Fresh Waters and Human Health
The Lake Erie Center for Fresh Waters and Human Health is a five-year, multi-institutional effort aimed at understanding the environmental factors and ongoing changes that influence the growth and toxicity of cyanobacterial harmful algal blooms (cHABs) in Lake Erie. The Center will support three research projects. Specifically these projects address the following aims: first, how environmental cues promote or constrain the proliferation of cHAB species in mixed populations; second, how environmental cues influence toxin production by cHAB species; third, how other member of the microbial assemblage influence cHAB growth and toxicity. The Center will provide a Community Engagement Core to lead outreach activities that will inform the general public on the effects of cHABs by efforts that include: (1) a community engaged scholarship training for scientists associated with the Center, (2) community-engaged scholarship training for practitioners or community members associated with the Center, and (3) a stakeholder needs assessment for Great Lakes and environmental health literacy to inform general outreach information needs. A citizen science engagement with charter boat captains will further develop a near real-time database on cHAB severity in Lake Erie, and the Facilities Core will provide metadata that not only serve the three stated research projects, but also yield a database available to all Great Lakes scientists. The outcomes are to involve community stakeholders and researchers in the Great Lakes on issues regarding human health, climate change and awareness of threats to our fresh water resources.
The vessel will come to a stop when the predetermined sampling station is reached, at a time decided on by the ship's command. The location and time of sampling will be recorded, as well as the environmental conditions. The trained crew members of the vessel will then use a YSI water quality sonde to collect data and that will be recorded. A Secchi disc with measurement indicators every 10 centimeters will then be deployed and recordings will be taken on both the upcast and downcast. A Van Dorn water sampler will then be used to collect water at a depth of 1 meter. Water samples are then transferred to 1 L polyethylene storage bottles or 2 L plastic storage bottles, and stored in a dark place at 4 oC until picked up by personnel on the same day. The samples will then be transported in coolers containing ice packs to Bowling Green State University (BGSU). Limited water samples were also taken from shore sampling locations, where the methodology remained the same.
Sub-samples for chlorophyll a will be taken at BGSU using 0.4 um polycarbonate membranes and a vacuum filtration system. The filter will be placed in a screw cap polyethylene centrifuge tube and stored in a dark freezer until extraction. The samples will be extracted using 90% acetone and kept at -20 oC for 24 hours, and chlorophyll will be measured using a TD-700 fluorometer (Welschmeyer, 1994).
Total and dissolved nutrient samples will be held in acid-washed 250 mL polyethylene bottles, and stored in a dark freezer at -20 oC until they are ready to be analyzed. Dissolved nutrient sub-samples will be taken by filtering the agitated sampled water through 0.22 um filters. The data will then be shipped to the National Center for Water Quality Research at Heidelberg University (Tiffin, OH).
The meteorological data was taken directly from readings using vessel instruments, marine forecasts, and expert observations. The physico-chemical data was obtained using a YSI 600QS multiparameter sonde, and nutrient concentrations were analyzed at the National Center for Water Quality Research at Heidelberg University. Fluorometric classification of phytoplankton was measured using a FluoroProbe (bbe Moldaenke GmbH, Schwentinental, Germany), and plankton taxonomic classification and cell enumeration was conducted by Aquatic Taxonomy Specialists (Malinta, OH). The extractive chlorophyll was read using a TD-700 Fluorometer (Turner Designs Inc., San Jose, CA), the sample concentrations were then calculated with the equation below, and the averages for each site were calculated from the triplicate samples.
Chla = ((reading*volumeextracted)/volumefiltered)* dilution factor
- Imported "Winter_Lake_Erie_2022_Metadata.csv" into BCO DMO system
- Combined "Date" and "Time" to create "ISO_DateTime_UTC" field in ISO UTC format
- Added flag field for ice collection; removed strings from sample depth column
- Replace "Pseudoa-bae-" with "Pseudanabaena"
- Modified parameter names to conform with BCO-DMO system requirements
- Removed "Ice_thickness_m" parameter as instructed by submitter
- Exported "940112_v1_lake_erie_winter_survey_2023-2024.csv" as main datafile and "winter_lake_erie_2023-2024_deployments.csv" as supplemental file
- Exported file as "949323_v1_winter_lake_erie_2022.csv"
| File |
|---|
949323_v1_winter_lake_erie_2022.csv (Comma Separated Values (.csv), 6.69 KB) MD5:a48b16b46bf81e949c907a0465ff99fa Primary data file for dataset ID 949323, version 1 |
| Parameter | Description | Units |
| Station | Station identifier | unitless |
| Date | Date of sample collection in YYYY-mm-dd format | unitless |
| Time | Time of sample collection in Eastern Standard Time in HH:MM format | unitless |
| ISO_DateTime_UTC | Datetime of sample collection in ISO 8601 format | unitless |
| Lake | Lake sampled | unitless |
| Lat | Latitude of sample collection, South is negative | decimal degrees |
| Long | Longitude of sample collection, West is negative | decimal degrees |
| Sample_depth | Depth of sample collection | meters (m) |
| Sample_ice_flag | Indicator that ice was collected as sample and melted for analysis; 1 = ice was collected; 0 = water was collected | unitless |
| Depth | Depth of water at sampling location | meters (m) |
| Air_temp | Air temperature | Degrees Celsius |
| Water_temp | Water temperature measured at surface | Degrees Celsius |
| Snow_thickness_profile | Depth of snow on the surface ice | meters (m) |
| Ice_thickness_in | Thickness of ice | inches (in) |
| Percent_trans_ice_snow | Light transmission through ice and snow | percent (%) |
| Percent_trans_ice | Light transmission through ice without snow cover | percent (%) |
| Ice_Narrative | Description of Ice | unitless |
| Snow_Narrative | Description of Snow | unitless |
| Wind_speed | Wind speed | Knots (Kt) |
| Wind_direction | Wind direction in cardinal (compass) directions | unitless |
| Barometer | Barometer reading | Hg |
| Chl_A | Average chlorophyll concentrations | ug/L |
| Cl | Average chloride concentration | ppm |
| NO2 | Average nitrite concentration | mg/L |
| TP | Average total dissolved phosphorus concentration | ug/L |
| TN | Average total dissolved nitrogen concentration | mg/L |
| NH4 | Average ammonium concentration | mg/L |
| Stephanodiscus_bieranus | Cell abundance: Stephanodiscus bieranus | Cells per milliliter (cells/mL) |
| Stephanodiscus_spp | Cell abundance: Stephanodiscus spp | Cells per milliliter (cells/mL) |
| Aulacoseira_spp | Cell abundance: Aulacoseira spp | Cells per milliliter (cells/mL) |
| Fragilaria_spp | Cell abundance: Fragilaria spp | Cells per milliliter (cells/mL) |
| Nitzschia_sp | Cell abundance: Nitzschia sp | Cells per milliliter (cells/mL) |
| Staurosira_spp | Cell abundance: Staurosira spp | Cells per milliliter (cells/mL) |
| Asterionella_formosa | Cell abundance: Asterionella formosa | Cells per milliliter (cells/mL) |
| Acinoccl_spp | Cell abundance: Acinoccl spp | Cells per milliliter (cells/mL) |
| Surirella_s | Cell abundance: Surirella s | Cells per milliliter (cells/mL) |
| Navicula_spp | Cell abundance: Navicula spp | Cells per milliliter (cells/mL) |
| Diatoma_spp | Cell abundance: Diatoma spp | Cells per milliliter (cells/mL) |
| Tabellaria_spp | Cell abundance: Tabellaria spp | Cells per milliliter (cells/mL) |
| Synedra_spp | Cell abundance: Synedra spp | Cells per milliliter (cells/mL) |
| Gomphonema_sp | Cell abundance: Gomphonema sp | Cells per milliliter (cells/mL) |
| Hippodonta_spp | Cell abundance: Hippodonta spp | Cells per milliliter (cells/mL) |
| Phyto_centric_small | Cell abundance: Phytoplankton, centric (<9 um) | Cells per milliliter (cells/mL) |
| Phyto_centric_large | Cell abundance: Phytoplankton, centric (>9 um) | Cells per milliliter (cells/mL) |
| Microflagellates | Cell abundance: Microflagellates | Cells per milliliter (cells/mL) |
| Cryptophyta | Cell abundance: Cryptophyta | Cells per milliliter (cells/mL) |
| Pyrrophyta | Cell abundance: Pyrrophyta | Cells per milliliter (cells/mL) |
| Chrysophyta | Cell abundance: Chrysophyta | Cells per milliliter (cells/mL) |
| Eugnophyta | Cell abundance: Eugnophyta | Cells per milliliter (cells/mL) |
| Scenedesmus_quadricatum | Cell abundance: Scenedesmus quadricatum | Cells per milliliter (cells/mL) |
| Tetrastrum_staurogeniaeforme | Cell abundance: Tetrastrum staurogeniaeforme | Cells per milliliter (cells/mL) |
| Micractinium_pusillum | Cell abundance: Micractinium pusillum | Cells per milliliter (cells/mL) |
| Ankistrodesmus_spp | Cell abundance: Ankistrodesmus spp | Cells per milliliter (cells/mL) |
| Actistrum_hantzschii | Cell abundance: Actistrum hantzschii | Cells per milliliter (cells/mL) |
| Chlorobiflagellates | Cell abundance: Chlorobiflagellates | Cells per milliliter (cells/mL) |
| Chlamydomos_spp | Cell abundance: Chlamydomos spp | Cells per milliliter (cells/mL) |
| Dictyosphaerium_spp | Cell abundance: Dictyosphaerium spp | Cells per milliliter (cells/mL) |
| Golenkenia_spp | Cell abundance: Golenkenia spp | Cells per milliliter (cells/mL) |
| Tetraedron_spp | Cell abundance: Tetraedron spp | Cells per milliliter (cells/mL) |
| coccoid_chlorophytes | Cell abundance: coccoid chlorophytes | Cells per milliliter (cells/mL) |
| Lagerhemia | Cell abundance: Lagerhemia | Cells per milliliter (cells/mL) |
| Closterium_spp | Cell abundance: Closterium spp | Cells per milliliter (cells/mL) |
| Pediastrum_spp | Cell abundance: Pediastrum spp | Cells per milliliter (cells/mL) |
| Selestrum_spp | Cell abundance: Selestrum spp | Cells per milliliter (cells/mL) |
| Oocystis_spp | Cell abundance: Oocystis spp | Cells per milliliter (cells/mL) |
| Spermatozopsis_spp | Cell abundance: Spermatozopsis spp | Cells per milliliter (cells/mL) |
| picoplankton | Cell abundance: picoplankton | Cells per milliliter (cells/mL) |
| Planktolyngbya_spp | Cell abundance: Planktolyngbya spp | Cells per milliliter (cells/mL) |
| Aphanothece_spp | Cell abundance: Aphanothece spp | Cells per milliliter (cells/mL) |
| Aphanocapsa_spp | Cell abundance: Aphanocapsa spp | Cells per milliliter (cells/mL) |
| Pseudanabaena | Cell abundance: Pseudanabaena | Cells per milliliter (cells/mL) |
| Microcystis_spp | Cell abundance: Microcystis spp | Cells per milliliter (cells/mL) |
| cocoid_cyanophyte | Cell abundance: cocoid cyanophyte | Cells per milliliter (cells/mL) |
| Chroococcus_spp | Cell abundance: Chroococcus spp | Cells per milliliter (cells/mL) |
| Dolichospermum_spp | Cell abundance: Dolichospermum spp | Cells per milliliter (cells/mL) |
| Merismopedia_spp | Cell abundance: Merismopedia spp | Cells per milliliter (cells/mL) |
| Aphanizomenon_flosaquae | Cell abundance: Aphanizomenon flosaquae | Cells per milliliter (cells/mL) |
| Dataset-specific Instrument Name | Sample bottles, 1 or 2 L (Nalgene or equivalent), Plastic wash bottle, 500 mL |
| Generic Instrument Name | Bottle |
| Dataset-specific Description | Water samples are then transferred to 1 L polyethylene storage bottles or 2 L plastic storage bottles, and stored in a dark place at 4 oC until picked up by personnel on the same day.
Total and dissolved nutrient samples will be held in acid-washed 250 mL polyethylene bottles, and stored in a dark freezer at -20 oC until they are ready to be analyzed. |
| Generic Instrument Description | A container, typically made of glass or plastic and with a narrow neck, used for storing drinks or other liquids. |
| Dataset-specific Instrument Name | FluoroProbe |
| Generic Instrument Name | Fluorometer |
| Dataset-specific Description | Fluorometric classification of phytoplankton was measured using a FluoroProbe (bbe Moldaenke GmbH, Schwentinental, Germany), and plankton taxonomic classification and cell enumeration was conducted by Aquatic Taxonomy Specialists (Malinta, OH). |
| Generic Instrument Description | A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. |
| Dataset-specific Instrument Name | National Center for Water Quality Research at Heidelberg University |
| Generic Instrument Name | Nutrient Autoanalyzer |
| Dataset-specific Description | Total and dissolved nutrient samples will be held in acid-washed 250 mL polyethylene bottles, and stored in a dark freezer at -20 oC until they are ready to be analyzed. Dissolved nutrient sub-samples will be taken by filtering the agitated sampled water through 0.22 um filters. The data will then be shipped to the National Center for Water Quality Research at Heidelberg University (Tiffin, OH). |
| Generic Instrument Description | Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. |
| Dataset-specific Instrument Name | Secchi disc |
| Generic Instrument Name | Secchi Disc |
| Dataset-specific Description | A Secchi disc with measurement indicators every 10 centimeters will then be deployed and recordings will be taken on both the upcast and downcast. |
| Generic Instrument Description | Typically, a 16 inch diameter white/black quadrant disc used to measure water optical clarity |
| Dataset-specific Instrument Name | TD-700 fluorometer |
| Generic Instrument Name | Turner Designs 700 Laboratory Fluorometer |
| Dataset-specific Description | The extractive chlorophyll was read using a TD-700 Fluorometer (Turner Designs Inc., San Jose, CA), the sample concentrations were then calculated with the equation below, and the averages for each site were calculated from the triplicate samples. |
| Generic Instrument Description | The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements. |
| Dataset-specific Instrument Name | Vacuum manifold system to accommodate 3 filter funnels |
| Generic Instrument Name | vacuum manifold |
| Dataset-specific Description | Vacuum manifold system to accommodate 3 filter funnels |
| Generic Instrument Description | A device that is used for the vacuum-driven processing of multiwell strips or plates, or spin columns. |
| Dataset-specific Instrument Name | 2 L Van Dorn water sampler |
| Generic Instrument Name | Van Dorn water sampler |
| Dataset-specific Description | A Van Dorn water sampler will then be used to collect water at a depth of 1 meter. |
| Generic Instrument Description | A free-flushing water sample bottle comprising a cylinder (polycarbonate, acrylic or PVC) with a stopper at each end. The bottle is closed by means of a messenger from the surface releasing the tension on a latex band and thus pulling the two stoppers firmly into place. A thermometer can be mounted inside the bottle. One or more bottles can be lowered on a line to allow sampling at a single or multiple depth levels. Van Dorn samplers are suitable for for physical (temperature), chemical and biological sampling in shallow to very deep water. Bottles are typically lowered vertically through the water column although a horizontal version is available for sampling near the seabed or at thermoclines or chemoclines. Because of the lack of metal parts the bottles are suitable for trace metal sampling, although the blue polyurethane seal used in the Alpha version may leach mercury. The Beta version uses white ASA plastic seals that do not leach mercury but are less durable. |
| Dataset-specific Instrument Name | YSI 600QS multiparameter sonde |
| Generic Instrument Name | YSI Sonde 6-Series |
| Dataset-specific Description | The physico-chemical data was obtained using a YSI 600QS multiparameter sonde, and nutrient concentrations were analyzed at the National Center for Water Quality Research at Heidelberg University. |
| Generic Instrument Description | YSI 6-Series water quality sondes and sensors are instruments for environmental monitoring and long-term deployments. YSI datasondes accept multiple water quality sensors (i.e., they are multiparameter sondes). Sondes can measure temperature, conductivity, dissolved oxygen, depth, turbidity, and other water quality parameters. The 6-Series includes several models. More from YSI. |
NSF Award Abstract:
The Lake Erie Center for Fresh Waters and Human Health is a five-year, multi-institutional effort aimed at understanding the environmental factors and ongoing changes that influence the growth and toxicity of cyanobacterial harmful algal blooms (cHABs) in Lake Erie. The Center will support three research projects. Specifically these projects address the following aims: first, how environmental cues promote or constrain the proliferation of cHAB species in mixed populations; second, how environmental cues influence toxin production by cHAB species; third, how other member of the microbial assemblage influence cHAB growth and toxicity. The Center will provide a Community Engagement Core to lead outreach activities that will inform the general public on the effects of cHABs by efforts that include: (1) a community engaged scholarship training for scientists associated with the Center, (2) community-engaged scholarship training for practitioners or community members associated with the Center, and (3) a stakeholder needs assessment for Great Lakes and environmental health literacy to inform general outreach information needs. A citizen science engagement with charter boat captains will further develop a near real-time database on cHAB severity in Lake Erie, and the Facilities Core will provide metadata that not only serve the three stated research projects, but also yield a database available to all Great Lakes scientists. The outcomes are to involve community stakeholders and researchers in the Great Lakes on issues regarding human health, climate change and awareness of threats to our fresh water resources.
The Center is jointly supported by NSF and by the National Institute for Environmental Health Sciences (NIEHS).
This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
| Funding Source | Award |
|---|---|
| NSF Division of Ocean Sciences (NSF OCE) |