Dataset: Metadata for longread sequencing of Carcinus maenas collected from Buzzards Bay, Massachusetts from May 2022 to Aug 2022

Samples of Carcinus maenas (urn:lsid:marinespecies.org:taxname:107381) were collected between May 2022 and Aug 2022 from Massachusetts waters. 

Extractions were performed with an NEB Monarch HMW DNA Extraction Kit for Cells & Blood (May runs; low success) or a Circulomics Nanobind Tissue Big DNA kit (June and August runs; good success). Library prep was performed with a Oxford Nanopore Cas9 sequencing kit and custom Cas9 probes targeting putative inversion regions. May and June runs used the same first-round set of probes, while August runs used an updated second-round set of probes. Probe sequences are included in the supplemental file and can be cross-referenced using the probe_set value, though targeting is imperfect so much of the data simply reflect non-targeted genome sequencing. Libraries were sequenced on a single flowcell of an Oxford Nanopore MinION mk1c. For each round of sequencing (May, June, and August), the same library was run twice for 24 hours each time, with a flowcell flush at 24 hours. The run_day value captures this, listing this pre- and post-flush runs as run_days 1 and 2, respectively, for each single sample.