Acartia tonsa were collected from Key Largo, Florida in February 2023. A portion of the adult female copepods in this collection were parasitized by juvenile bopyrid isopods. We measured thermal limits (as Critical Thermal Maximum) for individuals with and without these parasites. Data were collected by Dr. Matthew Sasaki.
Mature females were sorted from the bulk plankton tow. Females bearing juvenile bopyrid isopods were held separately from the unparasitized females. Thermal limits were measured as Critical Thermal Maxima (CTmax). During these assays, individual females are moved into 50 mL flat-bottom glass vials, which are then placed in a water bath. The temperature of the water bath is gradually raised (0.1-0.3°C per minute) until the individual stops moving. The water temperature at that point is recovered from a continuous temperature record; three sensors are placed into separate vials in the water bath and record temperature every five seconds for the duration of the experiment. These CTmax experiments were repeated four times over a three day period.
Organism identifiers (Life Science Identifier (LSID))
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943
bopyrid isopod, Bopyridae, urn:lsid:marinespecies.org:taxname:1195
Sasaki, M., Dam, H. G. (2025) Thermal limits of Acartia tonsa and the effect of isopod parasitism from Critical Thermal Maxima (CTmax) experiments conducted with copepods collected from Key Largo, Florida in February 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/955736 [access date]
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