Individual phytoplankton cells were collected on four cruises (March, May, August, and November 2019) as part of the Bermuda Atlantic Iron Time-Series (BAIT) Project. The elemental (Si, P, S, Mn, Fe, Co, Ni, Cu, Zn) content of each cell was measured with synchrotron x-ray fluorescence (SXRF). Carbon was calculated from biovolume. These data can be used to assess biogenic particulate metal fraction, as well as changes in the accumulation of these elements across seasons.
Samples were collected on 4 cruises (AE1909, AE1921, AE1930, and EN63) in the Western North Atlantic at the Bermuda Atlantic Time-Series Study region. Single-cell synchrotron x-ray fluorescence (SXRF) samples were collected from the surface mixed layer using the GEOTRACES rosette. Whole water samples were preserved with 0.25% trace-metal clean buffered glutaraldehyde (Twining et al., 2003) and centrifuged onto SiN TEM windows. Windows were briefly rinsed with a drop of ultrapure water and dried in a Class-100 cabinet. SXRF analysis was performed using the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory) following the protocols of Twining et al. (2011). Each cell was raster scanned with a focused 10 keV x-ray beam with a diameter of approximately 0.5 micrometers (um).
Twining, B. (2025) Element quotas of individual phytoplankton cells from four Bermuda Atlantic Iron Time-Series (BAIT) cruises in 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-19 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/956540 [access date]
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