Deployment: AE1516

Objectives:

1. TFF surface water samples to collect cells for two metabolite oxidation experiments: A) osmolytes, B) 14C-dimethylarsenate. 80 L surface sample to 600 ml, expecting ~10^7 cells ml.  This collection plan executed --- times over two days (late afternoon and morning, each day; (Giovannoni/Halsey experiments; Landry, Giovannoni on TFF).
2. TFF surface water samples to collect cells single cell genomics by FACS at JGI.  After concentration by TFF cells will be filtered through 0.45 um Nuclepore filters, and cryopreserved in SCGC glycine betaine cryopreservation buffer.
3. TFF 100 m water samples to collect cells for Ib, IIa, IIIa, IV or Vb ecotypes, TFF cell concentrates from will be filtered through 0.45 um Nuclepore filters, and cryopreserved in SCGC glycerol cryopreservation buffer.
4. DOM and nucleic acid water profile for chemistry and amplicon sequencing.  Surface to 300 m (surface, 40, 80, 120, 160, 200, 250, 300 m), all samples that will be first filtered through 0.2 µm Sterivex cartridges and then A) 300 ml will frozen at -80 (or -20, in a pinch) in polypropylene or teflon bottles; for chemistry, or B) 3.7 liter will be concentrated with PPL cartridges and stored at -20 in the bound state (sealed and store PPLs).  Add SLP to Sterivex cartridges and freeze at -80 C. DOC samples, collected in ashed EPA vials, will be collected for every DOM sample.

Cruise information and original data are available from the NSF R2R data catalog.