Instrument: Light-Dark Bottle

Acronym: Light-Dark Bottle

External Identifier: skos:broadMatch: http://vocab.nerc.ac.uk/collection/L05/current/82/

Description

The light/dark bottle is a way of measuring primary production by comparing before and after concentrations of dissolved oxygen.

Bottles containing seawater samples with phytoplankton are incubated for a predetermined period of time under light and dark conditions. Incubation is preferably carried out in situ, at the depth from which the samples were collected. Alternatively, the light and dark bottles are incubated in a water trough on deck, and neutral density filters are used to approximate the light conditions at the collection depth.

Rates of net and gross photosynthesis and respiration can be determined from measurements of dissolved oxygen concentration in the sample bottles.

Datasets (8)

Dataset Name	PI-Supplied Description	PI-Supplied Name
32Si and 14C production data (experimental) from EXPORTS cruise RR1813 on R/V Roger Revelle in the Subarctic North Pacific near Station PAPA from August to September 2018
32Si data from EXPORTS cruise RR1813 on R/V Roger Revelle in the Subarctic North Pacific near Station PAPA from August to September 2018
Primary productivity estimates from the incubation of seawater collected at the Bermuda Atlantic Time-series Study (BATS) site from December 1988 through June 2024	Seawater samples were collected prior to sunrise, separated into three light bottles and one dark bottle, and a radioactive 14C tracer added. The bottles were then deployed on an incubation array at their collection depths, and allowed to drift on the array for the full light day.	light and dark incubation bottles
Net primary productivity (14C) measurements made during quasi‐Lagrangian experiments on R/V Roger Revelle cruise RR2201 in the Argo basin in the Eastern Indian Ocean/Indonesian throughflow during February and March 2022		incubation bottles
Net community production and respiration rates from R/V Oceanus OC404-01, OC404-04 in the Sargasso Sea in2004 (EDDIES project)		Light-Dark Bottle
Oxygen evolution experiment data from R/V Oceanus OC404-01, OC404-04 in the Sargasso Sea in 2004 (EDDIES project)		Light-Dark Bottle
Gross and integrated gross oxygen productivity from R/V Atlantis II cruise AII-119-4 in the North Atlantic in 1989 (U.S. JGOFS NABE project)	100 ml quartz bottles were used to store samples which spiked with 0.2 ml of H2O(18) for 14 daylight hrs at the depth of collection.	Quartz Bottle
Size-fractioned primary production rates from samples collected on R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea Bermuda Atlantic Time-Series Station in 2011-2012 (Trophic BATS project)	Primary productivity was measured using light-dark bottles incubated in situ.	Light-Dark Bottle