Dataset: Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.2350.1Version 1 (2002-11-15)Dataset Type:Cruise Results

Principal Investigator: Chris H. Fritsen (Desert Research Institute)

BCO-DMO Data Manager: Ms Dicky Allison (Woods Hole Oceanographic Institution)


Program: U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)

Program: U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)

Project: U.S. GLOBEC Southern Ocean (SOGLOBEC)

Project: GLOBEC: Sea Ice Microbial Communities (Sea Ice Microbes)


Abstract

Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)

Bacteria Abundance, Biomass and Chlorophyll: Water Column Samplesa

BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination

Core Sampling techniques:

Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in 
Garrison and Buck (1986)

Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992)

Analytic Techniques:

Chla (mg m-3):

  • determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984)
  • ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting

Bacteria cell abundance (cells m-3) and biomass (mg C m-3):

LMG 0106

  • preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)
  • bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
  • bacteria biomass determined using cell abundance, cell biovolume (BV; mm3; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV0.86; Loferer-Krossbacher et al., 1998).
  • ice core samples corrected for FSW dilution

NBP 0104

  • preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)
  • bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
  • bacteria biomass determined using cell abundance, cell biovolume (BV; mm3), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV0.59; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data).
  • ice core samples corrected for FSW dilution

Data from LMG0106 (July-August, 2001) added in June 2002.

Updated: April 21, 2006


Related Datasets

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Related Publications

Methods

Garrison, D. L., & Buck, K. R. (1986). Organism losses during ice melting: A serious bias in sea ice community studies. Polar Biology, 6(4), 237–239. doi:10.1007/bf00443401
Methods

Horner, R., Ackley, S., Dieckmann, G., Gulliksen, B., Hoshiai, T., Legendre, L., … Sullivan, C. (1992). Ecology of sea ice biota. Polar Biology, 12(3-4). doi:10.1007/bf00243113
Methods

M. Loferer-Krößbacher, J. Klima, R. Psenner (1998) Determination of Bacterial Cell Dry Mass by Transmission Electron Microscopy and Densitometric Image Analysis. Applied and Environmental Microbiology, 64 (2) 688-694
Methods

Parsons, T. R., Y. Maita, and C. M. Lalli. "A Manual of Chemical and Biological Methods of Seawater Analysis", Pergamon Press (1984). ISBN: 9780080302874
Methods

Simon, M., & Azam, F. (1989). Protein content and protein synthesis rates of planktonic marine bacteria . Marine Ecology Progress Series, 51, 201–213. doi:10.3354/meps051201