Dataset: Crocosphaera watsonii CO2 and light experiment
Deployment: lab_Hutchins_07-12_diazotrophs

Growth, CO2- and N2-fixation of Crocosphaera watsonii in differing pCO2 and light levels.
Principal Investigator: 
David A. Hutchins (University of Southern California, USC)
Contact: 
Feixue Fu (University of Southern California, USC)
David A. Hutchins (University of Southern California, USC)
BCO-DMO Data Manager: 
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final no updates expected
Version: 
13 June 2013
Version Date: 
2013-06-13
Description

Results of laboratory experiments examining growth and CO2-fixation rate of two isolates of Crocosphaera watsonii, WH0401 and WH0402, in response to different pCO2 levels and light intensities. Isolates of C. watsonii, a unicellular marine N2-fixing cyanobacterium, were obtained from the western tropical Atlantic Ocean and cultured in the laboratory. WH0402 was grown at two concentrations of CO2 (air and 750 ppm) at five light intensities (18-300 umol quanta m-2 s-1). WH0401 was grown at three concentrations of CO2 (190, air, and 750 ppm) under the same range of light intensities (however WH0401 could not be acclimated to 18 or 50 umol quanta m-2 s-1).

Detailed methods and results are described in the following publication (see Figures 3, 4, and 5):
Garcia, N.S., Fu, F.X., Breene, C.L, Yu, E., Bernhardt, P.W., Mulholland, M.R., and Hutchins, D.A.  (2013).  Combined effects of CO2 and irradiance on the unicellular N2-fixing cyanobacterium Crocosphaera watsonii: a comparison of two isolates from the Western Tropical Atlantic Ocean. European Journal of Phycology 48: 128-139. DOI: 10.1080/09670262.2013.773383

Related datasets:
C watsonii light experiment
C watsonii CO2 experiment

More information about this dataset deployment