Dataset: Predicted regulon of the regulatory RNA ryhB in Vibrio fischeri (Iron regulation in Vibrio project)

ValidatedFinal no updates expectedVersion (2013-11-21)Dataset Type:Unknown

Principal Investigator: Dr Elizabeth Mann (Skidaway Institute of Oceanography)

Co-Principal Investigator: Dr Eric Stabb (University of Georgia)

Co-Principal Investigator: Dr Eberhard Voit (Georgia Institute of Technology)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Iron limitation, carbon metabolism and siderophore production in marine bacteria - a systems biology approach (Iron regulation in Vibrio)

An RNA-Seq experiment was performed in order to define the regulon of the small, regulatory RNA RyhB in Vibrio fischeri ES114. This was done by comparing the global gene expression in wildtype and ryhB mutant strains of V. fischeri. Since RyhB is de-repressed only under iron limiting conditions, this comparison must be performed when the level of intracellular iron is low.


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