Dataset: brooded coral larvae 3 - respiration raw data
Deployment: lab_Edmunds_NMMBA

Respiration of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013)

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Co-Principal Investigator:

Dr Vivian R Cumbo (California State University Northridge, CSUN)

Tung-Yung Fan (National Museum of Marine Biology and Aquarium, NMMBA)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Current State:

Final no updates expected

Version:

2014-10-07

Version Date:

2014-10-07

Data URL:

https://www.bco-dmo.org/dataset-deployment/535273/data

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Description

Methods & Sampling

Dataset acquisition description

Larval respiration was measured in a confined respirometer use a Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation usingwater-saturated air at the treatment temperature. Tomeasure respiration, 6 larvae were removed from the tubs in the treatment tanks and placed into 2-mL glass Wheaton vials filled with filtered seawater from the same treatment tank and sealed with Parafilm. A study conducted concurrently with the present analysis demonstrated that respiration of P. damicornis larvae in identical vials could be measured accurately with 5 larvae vial-1 (Edmunds et al., 2011).

Initial O2 concentration in the seawater was determined before the vials were sealed, and vials without larvae used as controls. Larvae in the sealed vials were incubated at their temperature treatments for 1.5-2 h in the dark using water baths set to the temperature of the respective treatments (±0.1 °C, Hipoint, models LC-06 and LC-10); vials were carefully moved throughout the incubations to ensure the seawater remained mixed. Incubation timeswere selected to ensure O2 concentrations remained >75% saturation. On completion of the incubations, vials were removed from the water baths, gently inverted to mix the seawater, and analyzed for O2 saturation. O2 saturation was converted to concentration using tabulated values of O2 solubility (N. Ramsing and J. Gundersen at http://www.unisense.com [based on Garcia and Gordon, 1992]) and the temperature and salinity of the seawater, and the change in O2 concentration used to calculate O2 flux after adjusting for controls. The respiration rates are expressed as nmol O2 mg protein-1 min-1.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

Data also available from PANGAEA: doi:10.1594/PANGAEA.823582

Data Processing Description

Dataset Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lab, lat, lon columns
- reformated date from d-Mon-yy to yyyy-mm-dd
- replaced blank cells with N_A
- replaced blanks and within cells with underscores
- changed Ambient to ambient; High to high; P. dam to Pocillopora_damicornis

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0844785	NSF Division of Ocean Sciences

Instruments

Aquarium chiller

Supplied Description:

Aquatech Ac11 or Shyeh Duwai Enterprise

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Gas Analyzer

Supplied Description:

Infra Red gas analyzer (S151, Qubit Systems)

Instrument Type

Generic Name: Gas Analyzer

Generic Description:

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Immersion heater

Supplied Description:

300 Wheaters, Taikong Corporation

Instrument Type

Generic Name: Immersion heater

Generic Description:

Submersible heating element for water tanks and aquaria.

LI-COR LI-192 PAR Sensor

Supplied Name: LI-COR LI-192 light sensor

Supplied Description:

cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400)

Instrument Type

Generic Name: LI-COR LI-192 PAR Sensor

Acronym: LI-COR LI-192 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/

Generic Description:

The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.

These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.

The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com)

Optode

Supplied Name: optrode

Supplied Description:

A Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature.

Instrument Type

Generic Name: Optode

Generic Description:

An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

Spectrophotometer

Supplied Name: spectrophotometer

Supplied Description:

USB2000, Ocean Optics

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Water Temperature Sensor

Supplied Name: Water Temp Sensor

Supplied Description:

certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C)

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	laboratory	unitless	laboratory
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
date	local date	yyyy-mm-dd	date
treatment	with or without larvae	unitless	treatment
sample	sample identification	unitless	sample
tank	tank id number	tank	tank
temp	target temperature	degrees Celsius	temp
treatment_pCO2	pCO2 treatment: ambient (47.5 - 49.3 Pa) or high (85.2 - 87.2 Pa)	unitless	treatment
days	days since start of experiment	unitless	days
num_larvae	number of larvae in respiration chamber	integer	count
species	coral species name	unitless	species
vol_chamber	the volume in which the cells were resuspended prior to counting	ml	sample_volume
time_inc	length of incubation	minutes	time_inc
O2_sat_pcent_start	oxygen saturation relative to air at start of incubation	percent	O2_sat_pcnt
O2_sat_pcent_final	oxygen saturation relative to air at end of incubation	percent	O2_sat_pcnt
resp_chamber	respiration rate (6 larvae/chamber)	umol O2/chamber/hr	O2_respir
resp_larv	respiration rate per larva	nmol O2/larva/min	O2_respir
resp_control	average respiration rate of the 4 control vials	micromol O2/chamber/hour	O2_respir
comment	comments	unitless	comment

Database

Contribute Data

Dataset: brooded coral larvae 3 - respiration raw data
Deployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: brooded coral larvae 3 - respiration raw dataDeployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Dataset: brooded coral larvae 3 - respiration raw data
Deployment: lab_Edmunds_NMMBA