Dataset: respiration and photosynthesis I
Deployment: MCR_Edmunds

Biomass-normalized dark respiration and net photosynthesis rates in coral and algae as a function of pCO2 tested in both 2011 and 2012, Moorea LTER site

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/645277

Co-Principal Investigator:

Robert Carpenter (California State University Northridge, CSUN)

Peter J. Edmunds (California State University Northridge, CSUN)

Contact:

Peter J. Edmunds (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Ocean Acidification and Coral Reefs: Scale Dependence and Adaptive Capacity (OA coral adaptation)

Current State:

Final no updates expected

Version:

Expand/Collapse All

Description

Related Reference: Comeau, S., Carpenter, R.C., Edmunds, P.J. Effects of pCO2 on photosynthesis and respiration of tropical scleractinian corals and calcified algae. ICES Journal of Marine Science doi:10.1093/icesjms/fsv267. Figures 1 and 2.

Methods & Sampling

Dataset acquisition description

Changes in dissolved oxygen in coral and algal samples were monitored during 30 min incubations with PreSens dipping oxygen optodes (PSt3) connected to Fibox 3 transmitters (Precision Sensing GmbH, Germany). The oxygen probes were calibrated every morning using a 2-point calibration in water-saturated air (100%) and anoxic seawater created by supersaturating seawater with sodium dithionite (Na2S2O4). Temperature was held constant during incubations by circulating water in a water jacket surrounding the incubation chamber. Chambers were positioned on magnetic stir plates, and seawater flow inside the chambers was regulated with magnetic stir bar that created vigorous and turbulent water motion. A similar irradiance to that used during incubations (i.e. 700 mmol quanta m22 s21) was maintained while the organisms were in the acrylic chambers by placing an LED lamp (75-W, Sol LED Module, Aquaillumination) above the chambers. On the same day that net photosynthesis was measured, and after at least 2 h of darkness (i.e. after 20:00 h), dark respiration was measured using an identical procedure to that used for photosynthesis, except the chamber was kept in darkness.

Data Processing Description

Dataset Processing Description

An Akaike information criterion (AIC) approach was used to determine if linear, logarithmic, or polynomial models best described the functional relationships of dark respiration, LDER, net photosynthesis, and gross photosynthesis against pCO2 for each species (see details in Comeau et al., 2013)

Comeau, S., Edmunds, P. J., Spindel, N. B., and Carpenter, R. C. 2013. The responses of eight coral reef calcifiers to increasing partial pressure of CO2 do not exhibit a tipping point. Limnology andOceanography, 58: 388–398.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added site, lab, lat, lon columns
- sorted by species, pCO2, and light_dark

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1415268	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Name:

Supplied Description:

open cell, potentiometric titrations (automatic titrator T50, Mettler-Toledo)

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Conductivity Meter

Supplied Name:

Supplied Description:

YSI 3100

Instrument Type

Generic Name: Conductivity Meter

Generic Description:

Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

LI-COR LI-193 PAR Sensor

Supplied Name:

Supplied Description:

4-pi quantum sensor LI-193 and a LiCor LI-1400 meter data logger.

Instrument Type

Generic Name: LI-COR LI-193 PAR Sensor

Acronym: LI-COR LI-193 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0458/

Generic Description:

The LI-193 Underwater Spherical Quantum Sensor uses a Silicon Photodiode and glass filters encased in a waterproof housing to measure PAR (in the 400 to 700 nm waveband) in aquatic environments. Typical output is in micromol s-1 m-2. The LI-193 Sensor gives an added dimension to underwater PAR measurements as it measures photon flux from all directions. This measurement is referred to as Photosynthetic Photon Flux Fluence Rate (PPFFR) or Quantum Scalar Irradiance. This is important, for example, when studying phytoplankton, which utilize radiation from all directions for photosynthesis. LI-COR began producing Spherical Quantum Sensors in 1979; serial numbers for the LI-193 begin with SPQA-XXXXX (licor.com).

Optode

Supplied Name:

Supplied Description:

PreSens dipping oxygen optodes (PSt3) connected to Fibox 3 transmitters (Precision Sensing GmbH, Germany).

Instrument Type

Generic Name: Optode

Generic Description:

An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

pH Sensor

Supplied Name:

Supplied Description:

Orion, 3-stars mobile mounted with a MettlerDG115-SCpHelectrode

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
site	location of experiments	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
taxon	taxonomic group	unitless	taxon
species	species name	unitless	species
pCO2	Partial pressure of carbon dioxide in the water body	uatm	pCO2
O2_change	Change in Oxygen concentration (normalization to surface area; cm-2)	ug O2/h/g	O2
O2_change_biomass	Change in Oxygen concentration (normalization to biomass; mg)	ug O2/h/g	O2
light_dark	whether incubation was in darkness or light	unitless	no_bcodmo_term

Database

Contribute Data

Dataset: respiration and photosynthesis I
Deployment: MCR_Edmunds

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: respiration and photosynthesis IDeployment: MCR_Edmunds

Dataset acquisition description

Dataset Processing Description

Dataset: respiration and photosynthesis I
Deployment: MCR_Edmunds