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Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.
Nutrients added to incubation: Control: no added nutrients N: Nitrogen as sodium nitrate, 20 umoles/L B1: Thiamine, 300 picomolar/L B7: Biotin, 100 picomoles/L B12: Cyanocobalamin, 100 picomoles/L All: Nitogen + Thiamine,+Cyanocobalamin, concentrations as above