Dataset: Grazing experiment 2 and 3: ingestion
Deployment: Lab_Olson_B

The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.

Expt. 2: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were fed to starved Favella taraikaensis cells for 15, 30 and 45 minutes. During each sampling time point, 20 mls of experiment volume was removed, fixed with glutaraldehyde and stained with DAPI. This volume was filtered onto a 20 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.

Expt. 3: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were fed to starved Oxyrrhis marina cells for 30, 60 and 90 minutes. During each sampling time point, 20 mls of experiment volume was removed, fixed with glutaraldehyde and stained with DAPI. This volume was filtered onto a 10 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.

Slides were evaluated under 1000x oil immersion using an epi-fluorescent microscope under blue-light excitation. The first 100 microzooplankton on each slide were assessed for each replicate/treatment, and individual prey cell were counted in the grazer food vacuole.