Dataset: Grazing experiment 4: cell size

Name: Grazing experiment 4: Cell size measurements of low-high pCO2 acclimated Rhodomonas (E Hux Response to pCO2 project)
Published: 2016-12-06
Keywords: biota, oceans

Cite This Dataset

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-12-06

Restricted:

Release Date:

2017-12-31

Validated:

Yes

Current State:

Final no updates expected

Grazing experiment 4: Cell size measurements of low-high pCO2 acclimated Rhodomonas (E Hux Response to pCO2 project)

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Description

Related Reference:

Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. On day 10, after Rhodomonas cells from the treatments were mounted live on a microscope slide and 50 cells from each treatment replicate were imaged using RSImage software under 400X magnification on an Olympus CHA microscope. ImageJ software was used to measure Rhodomonas length and width. Rhodomonas cells are described as having a prolate spheroid shape. The volume was calculated using: Vprolate(µm3) = (4/3) πa2b. Where a=1/2 width and b=1/2 length of the Rhodomonas cell

Data Processing Description

These data are unprocessed cell sizes as calculated above.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells
- added column for treatment to allow levelizing of data

Data Files

File
expt4_cell_size_day10.csv (Comma Separated Values (.csv), 20.80 KB) MD5:c10b1047172cdb036fe4eab2330a4b5a Primary data file for dataset ID 668794

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0961229

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Lab_Olson_B		2011-03-31	WWU		data info

Instruments

Microscope - Optical

Supplied Name: Olympus CHA microscope

Supplied Description:

Used to measure Rhodomonas cells

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment	treatment identifier: ambient, moderate, or high	unitless	treatment
Exp_day_treatment_rep_cell_number	sample identifier: individual cell measured: experiment day_pCO2 level_replicate_cell number	unitless	sample
length	cell length	micometers	length
width	cell width	micometers	width
volume	cell volume	micometers	volume

Status message

Database

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Dataset: Grazing experiment 4: cell size