Dataset: Grazing experiment 4: daily cell counts
Deployment: Lab_Olson_B

Daily cell count data for low-high pCO2 acclimated Rhodomonas sp.

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Data URL:

https://www.bco-dmo.org/dataset-deployment/668817/data

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Description

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

Dataset acquisition description

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were started and allowed to grow four days to reach a density of approximately 50,000 cells per ml. Cultures were then diluted daily with pre-equilibrated media containing f/50 nutrients. Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts. Cells were counted live on a Z2 Coulter Particle Counter. The dilution volume was then calculated to achieve a cell density of approximately 25,000 cells per ml which was the density determined in preliminary experiments to be adequate to maintain pCO2 near target concentrations.

Data Processing Description

Dataset Processing Description

These data are unprocessed cell counts.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961229	NSF Division of Ocean Sciences

Instruments

Coulter Counter

Supplied Name: Z2 Coulter Particle Counter

Supplied Description:

Used to count cells

Instrument Type

Generic Name: Coulter Counter

Generic Description:

An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.

from https://en.wikipedia.org/wiki/Coulter_counter

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment_replicate	sample identifier: individual grazer analyzed	unitless	sample
Day_1	Count for day 1 morning cell count	cells/milliliter	count
day_2	Count for day 2 morning cell count	cells/milliliter	count
day_2_post_dilution	Count for day 2 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
day_3	Count for day 3 morning cell count	cells/milliliter	count
day_3_post_dilution	Count for day 3 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
day_4	Count for day 4 morning cell count	cells/milliliter	count
day_4_post_dilution	Count for day 4 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
day_5	Count for day 5 morning cell count	cells/milliliter	count
day_5_post_dilution	Count for day 5 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
day_6	Count for day 6 morning cell count	cells/milliliter	count
day_6_post_dilution	Count for day 6 post-dilution; for the predicted cell count after dilution	cells/milliliter	count
day_7	Count for day 7 morning cell count	cells/milliliter	count

Database

Contribute Data

Dataset: Grazing experiment 4: daily cell counts
Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Grazing experiment 4: daily cell countsDeployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Dataset: Grazing experiment 4: daily cell counts
Deployment: Lab_Olson_B