Dataset: Grazing experiment 7: Carbon and Nitrogen
Deployment: Lab_Olson_B

Cellular carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Data URL:

https://www.bco-dmo.org/dataset-deployment/670074/data

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Description

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

Dataset acquisition description

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber (GF/F) filters. After filtration, filters were removed and placed in tin boats. Samples and controls (media blanks, filter blanks, and capsule blanks) were placed in a drying oven for 24 hours at 60 deg C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets, and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.

Data Processing Description

Dataset Processing Description

Picograms per cell for carbon and nitrogen were calculated based on standard curves and were then normalized to per cell based on cell counts for the sample day.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells
- replaced spaces with underscores

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961229	NSF Division of Ocean Sciences

Instruments

Elemental Analyzer

Supplied Name: Micro Cube elemental analyzer

Supplied Description:

Used to measure carbon and nitrogen concentrations

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: PDZ Europa 20-20 isotope ratio mass spectrometer

Supplied Description:

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
day_treatment_rep	Treatment replicate that names the sample and the day of semi-continuous culture	unitless	sample
C_ug	particulate carbon in sample	micrograms (ug)	TOC
C_pg	particulate carbon in sample	picograms (ug)	TOC
N_ug	particulate nitrogen in sample	micrograms (ug)	TON
N_pg	particulate nitrogen in sample	picograms (ug)	TON
total_cells	total number of cell on the filter	cells	cell_concentration
C_pg_cell	carbon per cell	picograms/cell (pg/cell)	TOC
N_pg_cell	nitrogen per cell	picograms/cell (pg/cell)	TON
C_to_N	ratio of carbon to nitrogen	dimensionless	C_to_N

Database

Contribute Data

Dataset: Grazing experiment 7: Carbon and Nitrogen
Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Grazing experiment 7: Carbon and NitrogenDeployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Dataset: Grazing experiment 7: Carbon and Nitrogen
Deployment: Lab_Olson_B