Dataset: TEM Pleurochrysis carterae virion images
Deployment: Bigelow_Martinez_2015-2016

A one-liter culture of P. carterae CCMP 645 in late exponential growth phase, grown in F/2 medium, was filtered through a 0.2 um PES membrane under sterile conditions. The filtrate was then initially concentrated down to approximately 25 ml by tangential flow filtration using a Vivaflow 50 cartridge (Sartorius) and further concentrated down to approximately 2 ml using a 30 kDa MWCO AmiconUltra-15 column (Millipore). Aliquots of the concentrate (2-20 ul) were spotted onto 200-mesh formvar/ carbon coated copper electron microscopy grids (Polysciences) and allowed to dry. Grids were then positively stained with filtered (0.2 um) 2% (wt/ vol) uranyl acetate (SPI) in de-ionized water (> 18  milliohm cm-2) for 2 min followed by removal of excess liquid via filter paper and a de-ionized water rinse. Grids were allowed to air-dry and were stored at room temperature prior to analysis. Imaging analysis was performed at the Applied Medical Sciences department at the University of Southern Maine, Portland, ME, USA using an FEI Technai TEM at magnifications 6,000x – 250,000x at 100 kV. Photographs were taken at magnifications between 11,000x and 180,000x.  

laboratory experiment