Dataset: 17D and isotopic ratios
Deployment: UpRISEE_SPOT_13-14

See complete methodology in Haskell et al. (2017). In summary:
This study is part of an effort aimed at characterizing the biological response to upwelling at SPOT on 21 cruises between January 2013 and June 2014; the Upwelling Regime In-Situ Ecosystem Efficiency (Up.R.I.S.E.E.) study.

O2/Ar and Triple Oxygen Isotopes (TOI): Samples for O2/Ar and TOI analysis were collected from Niskin bottles in 300 or 500 mL glass flasks equipped with airtight Louwers-Hapert valves (with three high-vacuum greased Viton O-rings in valve stem) and sidearms. Each bottle was poisoned with 150 uL saturated HgCl2, dried in an oven at 50 degrees C, preevacuated on a rotary vane vacuum pump (Pfeiffer Duo 2.5) for at least 5 min before evacuation on a stainless steel gas line using a turbo drag dry high vacuum pump (Alcatel Drytel 31). A vacuum of <1 mtorr was reached and held for at least 5 min, then the bottle was weighed. After sampling, the samples were equilibrated with the headspace for ~12–24 h on a shaking table at University of Southern California (USC), weighed, and drained. The headspace was analyzed in the laboratory of R. Stanley (Woods Hole Oceanographic Institution) for O2 and Ar by peak jumping and for 18O16O (m/z 34) and 17O16O (m/z 33) using a Thermofisher MAT 253 multicollector isotope ratio mass spectrometry. The TOI values are reported relative to atmospheric air as a standard, for which clean Woods Hole air stored in an electropolished 2 L stainless steel cylinder was used.